Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS

Nasir Hassan Wagini; Amira Shawky Soliman; Mohamed Said Abbas; Yasser Adel Hanafy; El-Saady Mohamed Badawy

doi:doi:10.11648/j.plant.20140203.11

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Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS

Nasir Hassan Wagini, Amira Shawky Soliman, Mohamed Said Abbas, Yasser Adel Hanafy, El-Saady Mohamed Badawy

Published in Plant (Volume 2, Issue 3)

Received: 25 March 2014 Accepted: 16 April 2014 Published: 10 May 2014

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Abstract

This study designed to compare phytochemicals for both Nigerian and Egyptian henna using preliminary test for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol, and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample. Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone (2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus, the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.

Published in	Plant (Volume 2, Issue 3)
DOI	10.11648/j.plant.20140203.11
Page(s)	27-32
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Henna, Lawsonia Inermis, Phytochemicals, Nigeria, Egypt

References

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[9]	R. Hema, S. Kumaravel and N. Gomathi, “Gas Chromatography Mass Spectroscopic analysis of Lawsonia inermis Leaves,” New York Science Journal, 2010. 3(12): 99-101.
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[13]	H. Kawo and A. M. Kwa, “Phytochemical screening and antibacterial activity of the aqueous extracts and frac-tions of ethanolic extracts of Lawsonia inermis Leaf,” International Research Journal of Microbi-ology, 2011, 2(12): 510-516.
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[15]	Priyanka P. and I. Rakesh, andR. Upadhyay “Physico-chemical and preliminary phytochemical screening of Psoralea corylifolia,” Arch. Appl. Sci. Res, 2013, 5 (2):261-265.
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APA Style

Nasir Hassan Wagini, Amira Shawky Soliman, Mohamed Said Abbas, Yasser Adel Hanafy, El-Saady Mohamed Badawy. (2014). Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS. Plant, 2(3), 27-32. https://doi.org/10.11648/j.plant.20140203.11

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ACS Style

Nasir Hassan Wagini; Amira Shawky Soliman; Mohamed Said Abbas; Yasser Adel Hanafy; El-Saady Mohamed Badawy. Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS. Plant. 2014, 2(3), 27-32. doi: 10.11648/j.plant.20140203.11

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AMA Style

Nasir Hassan Wagini, Amira Shawky Soliman, Mohamed Said Abbas, Yasser Adel Hanafy, El-Saady Mohamed Badawy. Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS. Plant. 2014;2(3):27-32. doi: 10.11648/j.plant.20140203.11

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@article{10.11648/j.plant.20140203.11,
  author = {Nasir Hassan Wagini and Amira Shawky Soliman and Mohamed Said Abbas and Yasser Adel Hanafy and El-Saady Mohamed Badawy},
  title = {Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS},
  journal = {Plant},
  volume = {2},
  number = {3},
  pages = {27-32},
  doi = {10.11648/j.plant.20140203.11},
  url = {https://doi.org/10.11648/j.plant.20140203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20140203.11},
  abstract = {This study designed to compare phytochemicals for both Nigerian and Egyptian henna using preliminary test for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol, and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample. Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone (2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus, the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.},
 year = {2014}
}

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TY - JOUR
T1 - Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS
AU - Nasir Hassan Wagini
AU - Amira Shawky Soliman
AU - Mohamed Said Abbas
AU - Yasser Adel Hanafy
AU - El-Saady Mohamed Badawy
Y1 - 2014/05/10
PY - 2014
N1 - https://doi.org/10.11648/j.plant.20140203.11
DO - 10.11648/j.plant.20140203.11
T2 - Plant
JF - Plant
JO - Plant
SP - 27
EP - 32
PB - Science Publishing Group
SN - 2331-0677
UR - https://doi.org/10.11648/j.plant.20140203.11
AB - This study designed to compare phytochemicals for both Nigerian and Egyptian henna using preliminary test for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol, and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample. Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone (2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus, the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.
VL - 2
IS - 3
ER -

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Author Information

Nasir Hassan Wagini

Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria
Amira Shawky Soliman

Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt
Mohamed Said Abbas

Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt
Yasser Adel Hanafy

Medical and Aromatic Plants Unit, Desert Research Center, Cairo, Egypt
El-Saady Mohamed Badawy

Medicinal and Ornamental Plant, Faculty of Agriculture, Cairo University, Cairo, Egypt

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APA Style

Nasir Hassan Wagini, Amira Shawky Soliman, Mohamed Said Abbas, Yasser Adel Hanafy, El-Saady Mohamed Badawy. (2014). Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS. Plant, 2(3), 27-32. https://doi.org/10.11648/j.plant.20140203.11

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ACS Style

Nasir Hassan Wagini; Amira Shawky Soliman; Mohamed Said Abbas; Yasser Adel Hanafy; El-Saady Mohamed Badawy. Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS. Plant. 2014, 2(3), 27-32. doi: 10.11648/j.plant.20140203.11

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AMA Style

Nasir Hassan Wagini, Amira Shawky Soliman, Mohamed Said Abbas, Yasser Adel Hanafy, El-Saady Mohamed Badawy. Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS. Plant. 2014;2(3):27-32. doi: 10.11648/j.plant.20140203.11

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@article{10.11648/j.plant.20140203.11,
  author = {Nasir Hassan Wagini and Amira Shawky Soliman and Mohamed Said Abbas and Yasser Adel Hanafy and El-Saady Mohamed Badawy},
  title = {Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS},
  journal = {Plant},
  volume = {2},
  number = {3},
  pages = {27-32},
  doi = {10.11648/j.plant.20140203.11},
  url = {https://doi.org/10.11648/j.plant.20140203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20140203.11},
  abstract = {This study designed to compare phytochemicals for both Nigerian and Egyptian henna using preliminary test for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol, and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample. Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone (2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus, the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.},
 year = {2014}
}

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