Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats

Venkatanarayana Gangarapu; Sudhakara Gujjala; Rajeswaramma Korivi; Indira Pala

doi:doi:10.11648/j.ajls.20130103.17

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Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats

Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala

Published in American Journal of Life Sciences (Volume 1, Issue 3)

Received: 23 May 2013 Published: 20 June 2013

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Abstract

The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.

Published in	American Journal of Life Sciences (Volume 1, Issue 3)
DOI	10.11648/j.ajls.20130103.17
Page(s)	117-124
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Curcumin, Carbon Tetrachloride, Oxidative Stress, Vitamin E, Silymarin

References

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APA Style

Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala. (2013). Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. American Journal of Life Sciences, 1(3), 117-124. https://doi.org/10.11648/j.ajls.20130103.17

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ACS Style

Venkatanarayana Gangarapu; Sudhakara Gujjala; Rajeswaramma Korivi; Indira Pala. Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. Am. J. Life Sci. 2013, 1(3), 117-124. doi: 10.11648/j.ajls.20130103.17

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AMA Style

Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala. Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. Am J Life Sci. 2013;1(3):117-124. doi: 10.11648/j.ajls.20130103.17

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@article{10.11648/j.ajls.20130103.17,
  author = {Venkatanarayana Gangarapu and Sudhakara Gujjala and Rajeswaramma Korivi and Indira Pala},
  title = {Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {3},
  pages = {117-124},
  doi = {10.11648/j.ajls.20130103.17},
  url = {https://doi.org/10.11648/j.ajls.20130103.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130103.17},
  abstract = {The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of  CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.},
 year = {2013}
}

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TY  - JOUR
T1  - Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats
AU  - Venkatanarayana Gangarapu
AU  - Sudhakara Gujjala
AU  - Rajeswaramma Korivi
AU  - Indira Pala
Y1  - 2013/06/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajls.20130103.17
DO  - 10.11648/j.ajls.20130103.17
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 117
EP  - 124
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20130103.17
AB  - The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of  CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.
VL  - 1
IS  - 3
ER  -

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Author Information

Venkatanarayana Gangarapu

Department of Zoology, Sri Krishnadevaraya University, Anantapur.
Sudhakara Gujjala

Department of Biochemistry, Sri Krishnadevaraya University, Anantapur
Rajeswaramma Korivi

Department of Chemistry, Sri Venkateswara University, Tirupati
Indira Pala

Department of Zoology, Sri Krishnadevaraya University, Anantapur.

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Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala. (2013). Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. American Journal of Life Sciences, 1(3), 117-124. https://doi.org/10.11648/j.ajls.20130103.17

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ACS Style

Venkatanarayana Gangarapu; Sudhakara Gujjala; Rajeswaramma Korivi; Indira Pala. Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. Am. J. Life Sci. 2013, 1(3), 117-124. doi: 10.11648/j.ajls.20130103.17

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AMA Style

Venkatanarayana Gangarapu, Sudhakara Gujjala, Rajeswaramma Korivi, Indira Pala. Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats. Am J Life Sci. 2013;1(3):117-124. doi: 10.11648/j.ajls.20130103.17

Copy | Download

@article{10.11648/j.ajls.20130103.17,
  author = {Venkatanarayana Gangarapu and Sudhakara Gujjala and Rajeswaramma Korivi and Indira Pala},
  title = {Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {3},
  pages = {117-124},
  doi = {10.11648/j.ajls.20130103.17},
  url = {https://doi.org/10.11648/j.ajls.20130103.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130103.17},
  abstract = {The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of  CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.},
 year = {2013}
}

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TY  - JOUR
T1  - Combined Effect of Curcumin and Vitamin E against CCl4 Induced Liver Injury in Rats
AU  - Venkatanarayana Gangarapu
AU  - Sudhakara Gujjala
AU  - Rajeswaramma Korivi
AU  - Indira Pala
Y1  - 2013/06/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajls.20130103.17
DO  - 10.11648/j.ajls.20130103.17
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 117
EP  - 124
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20130103.17
AB  - The present study was aimed to investigate the combined effect of curcumin[CMN] and vitamin E against CCl4 induced oxidative stress and liver toxicity in rats. The toxicant CCl4 was used to induce liver toxicity at a dose of 1ml/kg as 1: 1 (v/v) mixture with liquid parafiin twice weekly for 8 weeks and sylamarinwas used as a standard drug to compare hepatoprotective effects of CMN and vitamin E. The antifibrotic effects of CMN and vitamin E were assessed directly by hepatic histology and indirectly by measuring levels of the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lipid profile (total cholesterol and triglycerides) in serum. Further, we investigated lipid peroxidation (TBARS), enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase) and non-enzymatic antioxidant glutathione (GSH) were estimated in the liver samples. Oral administration of  CMN(100mg/kg daily for 8 weeks) and in combination with vitamin E (40mg/kg thrice weekly for 8 weeks) showed significant hepatoprotection by decreasing elevated serum markers and lipid profile. In addition significantly decreased the hepatic lipid peroxidation as well as recovered the activities of antioxidant enzymes and GSH levels in the liver. Histopathological studies confirmed the biochemical observations. This study reveals CMN in combination with vitamin E possesses strong antioxidant and hepatoprotective activity than CMN alone.
VL  - 1
IS  - 3
ER  -

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