Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings

Stanley Chukwudozie Onuoha; Edna Ifeoma Chukwura; Kayode Fatokun

doi:doi:10.11648/j.ajbio.20140201.14

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Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings

Stanley Chukwudozie Onuoha, Edna Ifeoma Chukwura, Kayode Fatokun

Published in American Journal of BioScience (Volume 2, Issue 1)

Received: 8 December 2013 Published: 10 January 2014

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Abstract

The potentials of organic wastes from animal droppings as bioremediation alternative for soils spiked with waste-lubricating motor oil (spent oil) was evaluated. The rate of biodegradation of the spent oil was studied for a period of 6 months under laboratory condition. The result of the microbial counts for soils spiked with 5000 mgkg-1(0.5%) spent oil has its total heterotrophic bacterial count in unamended control soil ranging from 2.71±0.09 to 7.21±0.25 x 106 CFU/g of soil, while those of soil amended with cow droppings (CD) ranged from 16.0±1.01 to 47.90±0.36x 106 CFU/g of soil and those of soil amended with goat droppings (GD) and poultry manure(PM) ranged from 16.6±0.6 to 57.9±0.15 x 106 and 18.00±0.20×106 to 60.80± 1.19 ×106 CFU/g of soil respectively. The counts of hydrocarbon-utilizing bacteria (HUB) in unamended control soil ranged from 0.93±0.11 to 2.10±0.15 ×106 CFU/g of soil. The count of HUB in PM amended soil was significantly higher than those amended with goat droppings (GD), while those of goat dropping were higher than those of cow dung. The HUB count in GD amended soil ranged from 8.07± 0.12x106 to 67.70±0.68 x 106 cfu/g of soil. The THB and HUB counts for soils spiked with 25000 mg/kg (2.5%) spent oil exhibited a similar trend as was observed for soil spiked with 0.5% spent oil. Evaluation by the first-order kinetic model which utilized combined data for the entire period revealed that PM with biodegradation rate constant of 0.2332 day-1 and half-life of 2.97days was better in stimulating biodegradation of oil at higher concentration, while GD with a biodegradation constant of 0.3253day-1 and half-life of 2.13 days performed better at low pollution when compared to that of PM and CD.

Published in	American Journal of BioScience (Volume 2, Issue 1)
DOI	10.11648/j.ajbio.20140201.14
Page(s)	19-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Biodegradation, Waste-Lubricating Oil, Bacteria, Organic Waste, Hydrocarbon

References

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APA Style

Stanley Chukwudozie Onuoha, Edna Ifeoma Chukwura, Kayode Fatokun. (2014). Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings. American Journal of BioScience, 2(1), 19-27. https://doi.org/10.11648/j.ajbio.20140201.14

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ACS Style

Stanley Chukwudozie Onuoha; Edna Ifeoma Chukwura; Kayode Fatokun. Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings. Am. J. BioScience 2014, 2(1), 19-27. doi: 10.11648/j.ajbio.20140201.14

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AMA Style

Stanley Chukwudozie Onuoha, Edna Ifeoma Chukwura, Kayode Fatokun. Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings. Am J BioScience. 2014;2(1):19-27. doi: 10.11648/j.ajbio.20140201.14

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@article{10.11648/j.ajbio.20140201.14,
  author = {Stanley Chukwudozie Onuoha and Edna Ifeoma Chukwura and Kayode Fatokun},
  title = {Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {1},
  pages = {19-27},
  doi = {10.11648/j.ajbio.20140201.14},
  url = {https://doi.org/10.11648/j.ajbio.20140201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140201.14},
  abstract = {The potentials of organic wastes from animal droppings as bioremediation alternative for soils spiked with waste-lubricating motor oil (spent oil) was evaluated. The rate of biodegradation of the spent oil was studied for a period of 6 months under laboratory condition. The result of the microbial counts for soils spiked with 5000 mgkg-1(0.5%) spent oil has its total heterotrophic bacterial count in  unamended control soil ranging from 2.71±0.09 to 7.21±0.25 x 106 CFU/g  of soil, while those of soil amended with cow droppings (CD) ranged from 16.0±1.01 to 47.90±0.36x 106 CFU/g of soil and those of soil amended with goat droppings (GD) and  poultry manure(PM) ranged from 16.6±0.6 to 57.9±0.15 x 106 and 18.00±0.20×106  to 60.80± 1.19 ×106  CFU/g of soil respectively. The counts of hydrocarbon-utilizing bacteria (HUB) in unamended control soil ranged from 0.93±0.11 to 2.10±0.15 ×106 CFU/g of soil. The count of HUB in PM amended soil was significantly higher than those amended with goat droppings (GD), while those of goat dropping were higher than those of cow dung. The HUB count in GD amended soil ranged from 8.07± 0.12x106  to 67.70±0.68 x 106 cfu/g of soil. The THB and HUB counts for soils spiked with 25000 mg/kg (2.5%) spent oil exhibited a similar trend as was observed for soil spiked with 0.5% spent oil. Evaluation by the first-order kinetic model which utilized combined data for the entire period revealed that PM with biodegradation rate constant of 0.2332 day-1 and half-life of 2.97days was better in stimulating biodegradation of oil at higher concentration, while GD with a biodegradation constant of 0.3253day-1 and half-life of 2.13 days performed better at low pollution when compared to that of PM and CD.},
 year = {2014}
}

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TY  - JOUR
T1  - Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings
AU  - Stanley Chukwudozie Onuoha
AU  - Edna Ifeoma Chukwura
AU  - Kayode Fatokun
Y1  - 2014/01/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140201.14
DO  - 10.11648/j.ajbio.20140201.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 19
EP  - 27
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140201.14
AB  - The potentials of organic wastes from animal droppings as bioremediation alternative for soils spiked with waste-lubricating motor oil (spent oil) was evaluated. The rate of biodegradation of the spent oil was studied for a period of 6 months under laboratory condition. The result of the microbial counts for soils spiked with 5000 mgkg-1(0.5%) spent oil has its total heterotrophic bacterial count in  unamended control soil ranging from 2.71±0.09 to 7.21±0.25 x 106 CFU/g  of soil, while those of soil amended with cow droppings (CD) ranged from 16.0±1.01 to 47.90±0.36x 106 CFU/g of soil and those of soil amended with goat droppings (GD) and  poultry manure(PM) ranged from 16.6±0.6 to 57.9±0.15 x 106 and 18.00±0.20×106  to 60.80± 1.19 ×106  CFU/g of soil respectively. The counts of hydrocarbon-utilizing bacteria (HUB) in unamended control soil ranged from 0.93±0.11 to 2.10±0.15 ×106 CFU/g of soil. The count of HUB in PM amended soil was significantly higher than those amended with goat droppings (GD), while those of goat dropping were higher than those of cow dung. The HUB count in GD amended soil ranged from 8.07± 0.12x106  to 67.70±0.68 x 106 cfu/g of soil. The THB and HUB counts for soils spiked with 25000 mg/kg (2.5%) spent oil exhibited a similar trend as was observed for soil spiked with 0.5% spent oil. Evaluation by the first-order kinetic model which utilized combined data for the entire period revealed that PM with biodegradation rate constant of 0.2332 day-1 and half-life of 2.97days was better in stimulating biodegradation of oil at higher concentration, while GD with a biodegradation constant of 0.3253day-1 and half-life of 2.13 days performed better at low pollution when compared to that of PM and CD.
VL  - 2
IS  - 1
ER  -

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Author Information

Stanley Chukwudozie Onuoha

Department of Biotechnology, Ebonyi State University, PMB 053 Abakaliki, Ebonyi State, Nigeria
Edna Ifeoma Chukwura

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Kayode Fatokun

Department of Agriculture, University of Zululand, PMB X1001 KwaDlangezwa 3886, South Africa

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Plain Text BibTeX RIS

APA Style

Stanley Chukwudozie Onuoha, Edna Ifeoma Chukwura, Kayode Fatokun. (2014). Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings. American Journal of BioScience, 2(1), 19-27. https://doi.org/10.11648/j.ajbio.20140201.14

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ACS Style

Stanley Chukwudozie Onuoha; Edna Ifeoma Chukwura; Kayode Fatokun. Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings. Am. J. BioScience 2014, 2(1), 19-27. doi: 10.11648/j.ajbio.20140201.14

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AMA Style

Stanley Chukwudozie Onuoha, Edna Ifeoma Chukwura, Kayode Fatokun. Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings. Am J BioScience. 2014;2(1):19-27. doi: 10.11648/j.ajbio.20140201.14

Copy | Download

@article{10.11648/j.ajbio.20140201.14,
  author = {Stanley Chukwudozie Onuoha and Edna Ifeoma Chukwura and Kayode Fatokun},
  title = {Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {1},
  pages = {19-27},
  doi = {10.11648/j.ajbio.20140201.14},
  url = {https://doi.org/10.11648/j.ajbio.20140201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140201.14},
  abstract = {The potentials of organic wastes from animal droppings as bioremediation alternative for soils spiked with waste-lubricating motor oil (spent oil) was evaluated. The rate of biodegradation of the spent oil was studied for a period of 6 months under laboratory condition. The result of the microbial counts for soils spiked with 5000 mgkg-1(0.5%) spent oil has its total heterotrophic bacterial count in  unamended control soil ranging from 2.71±0.09 to 7.21±0.25 x 106 CFU/g  of soil, while those of soil amended with cow droppings (CD) ranged from 16.0±1.01 to 47.90±0.36x 106 CFU/g of soil and those of soil amended with goat droppings (GD) and  poultry manure(PM) ranged from 16.6±0.6 to 57.9±0.15 x 106 and 18.00±0.20×106  to 60.80± 1.19 ×106  CFU/g of soil respectively. The counts of hydrocarbon-utilizing bacteria (HUB) in unamended control soil ranged from 0.93±0.11 to 2.10±0.15 ×106 CFU/g of soil. The count of HUB in PM amended soil was significantly higher than those amended with goat droppings (GD), while those of goat dropping were higher than those of cow dung. The HUB count in GD amended soil ranged from 8.07± 0.12x106  to 67.70±0.68 x 106 cfu/g of soil. The THB and HUB counts for soils spiked with 25000 mg/kg (2.5%) spent oil exhibited a similar trend as was observed for soil spiked with 0.5% spent oil. Evaluation by the first-order kinetic model which utilized combined data for the entire period revealed that PM with biodegradation rate constant of 0.2332 day-1 and half-life of 2.97days was better in stimulating biodegradation of oil at higher concentration, while GD with a biodegradation constant of 0.3253day-1 and half-life of 2.13 days performed better at low pollution when compared to that of PM and CD.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Stimulated Biodegradation of Spent Lubricating Motor Oil in Soil Amended with Animal Droppings
AU  - Stanley Chukwudozie Onuoha
AU  - Edna Ifeoma Chukwura
AU  - Kayode Fatokun
Y1  - 2014/01/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140201.14
DO  - 10.11648/j.ajbio.20140201.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 19
EP  - 27
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140201.14
AB  - The potentials of organic wastes from animal droppings as bioremediation alternative for soils spiked with waste-lubricating motor oil (spent oil) was evaluated. The rate of biodegradation of the spent oil was studied for a period of 6 months under laboratory condition. The result of the microbial counts for soils spiked with 5000 mgkg-1(0.5%) spent oil has its total heterotrophic bacterial count in  unamended control soil ranging from 2.71±0.09 to 7.21±0.25 x 106 CFU/g  of soil, while those of soil amended with cow droppings (CD) ranged from 16.0±1.01 to 47.90±0.36x 106 CFU/g of soil and those of soil amended with goat droppings (GD) and  poultry manure(PM) ranged from 16.6±0.6 to 57.9±0.15 x 106 and 18.00±0.20×106  to 60.80± 1.19 ×106  CFU/g of soil respectively. The counts of hydrocarbon-utilizing bacteria (HUB) in unamended control soil ranged from 0.93±0.11 to 2.10±0.15 ×106 CFU/g of soil. The count of HUB in PM amended soil was significantly higher than those amended with goat droppings (GD), while those of goat dropping were higher than those of cow dung. The HUB count in GD amended soil ranged from 8.07± 0.12x106  to 67.70±0.68 x 106 cfu/g of soil. The THB and HUB counts for soils spiked with 25000 mg/kg (2.5%) spent oil exhibited a similar trend as was observed for soil spiked with 0.5% spent oil. Evaluation by the first-order kinetic model which utilized combined data for the entire period revealed that PM with biodegradation rate constant of 0.2332 day-1 and half-life of 2.97days was better in stimulating biodegradation of oil at higher concentration, while GD with a biodegradation constant of 0.3253day-1 and half-life of 2.13 days performed better at low pollution when compared to that of PM and CD.
VL  - 2
IS  - 1
ER  -

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