Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq

Abdulsadah A. Rahi; Sundus Nsaif; Jaafar J. Hassoni; Magda A. Ali; Hassan A. Hamza

doi:doi:10.11648/j.ajbio.20130101.11

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Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq

Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza

Published in American Journal of BioScience (Volume 1, Issue 1)

Received: 14 April 2013 Published: 2 May 2013

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Abstract

Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.

Published in	American Journal of BioScience (Volume 1, Issue 1)
DOI	10.11648/j.ajbio.20130101.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Cutaneous Leishmaniasis, PCR, Diagnosis

References

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APA Style

Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza. (2013). Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. American Journal of BioScience, 1(1), 1-5. https://doi.org/10.11648/j.ajbio.20130101.11

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ACS Style

Abdulsadah A. Rahi; Sundus Nsaif; Jaafar J. Hassoni; Magda A. Ali; Hassan A. Hamza. Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. Am. J. BioScience 2013, 1(1), 1-5. doi: 10.11648/j.ajbio.20130101.11

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AMA Style

Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza. Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. Am J BioScience. 2013;1(1):1-5. doi: 10.11648/j.ajbio.20130101.11

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@article{10.11648/j.ajbio.20130101.11,
  author = {Abdulsadah A. Rahi and Sundus Nsaif and Jaafar J. Hassoni and Magda A. Ali and Hassan A. Hamza},
  title = {Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajbio.20130101.11},
  url = {https://doi.org/10.11648/j.ajbio.20130101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130101.11},
  abstract = {Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.},
 year = {2013}
}

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TY  - JOUR
T1  - Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq
AU  - Abdulsadah A. Rahi
AU  - Sundus Nsaif
AU  - Jaafar J. Hassoni
AU  - Magda A. Ali
AU  - Hassan A. Hamza
Y1  - 2013/05/02
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130101.11
DO  - 10.11648/j.ajbio.20130101.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130101.11
AB  - Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.
VL  - 1
IS  - 1
ER  -

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Author Information

Abdulsadah A. Rahi

College of Science/Wasit University, Iraq
Sundus Nsaif

College of Medicine/Kufa University, Iraq
Jaafar J. Hassoni

College of Medicine/Wasit University, Iraq
Magda A. Ali

College of Medicine/Wasit University, Iraq, TUMS-IC
Hassan A. Hamza

Al-Sadir Hospital/Ministry of Health, Iraq

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Plain Text BibTeX RIS

APA Style

Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza. (2013). Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. American Journal of BioScience, 1(1), 1-5. https://doi.org/10.11648/j.ajbio.20130101.11

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ACS Style

Abdulsadah A. Rahi; Sundus Nsaif; Jaafar J. Hassoni; Magda A. Ali; Hassan A. Hamza. Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. Am. J. BioScience 2013, 1(1), 1-5. doi: 10.11648/j.ajbio.20130101.11

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AMA Style

Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza. Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq. Am J BioScience. 2013;1(1):1-5. doi: 10.11648/j.ajbio.20130101.11

Copy | Download

@article{10.11648/j.ajbio.20130101.11,
  author = {Abdulsadah A. Rahi and Sundus Nsaif and Jaafar J. Hassoni and Magda A. Ali and Hassan A. Hamza},
  title = {Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajbio.20130101.11},
  url = {https://doi.org/10.11648/j.ajbio.20130101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130101.11},
  abstract = {Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.},
 year = {2013}
}

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TY  - JOUR
T1  - Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq
AU  - Abdulsadah A. Rahi
AU  - Sundus Nsaif
AU  - Jaafar J. Hassoni
AU  - Magda A. Ali
AU  - Hassan A. Hamza
Y1  - 2013/05/02
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130101.11
DO  - 10.11648/j.ajbio.20130101.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130101.11
AB  - Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.
VL  - 1
IS  - 1
ER  -

Copy | Download