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Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria

Received: 27 April 2013     Published: 2 May 2013
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Abstract

Antibacterial activity of ethanolic and aqueous extracts of Capsella Bursa were carried out against eight different species of bacteria, Gram-positive Staphylococcus aureus and Enterococcus fecalis and Gram-negative Escherichia coli, Proteus vulgaris, Serratia marcescens,Acinitobacter bumani, Klebsiella pneumoniae and Pseudomonas aeruginosa. It is active antibacterial only against gram-negative bacteria. The ethanolic and aqueous extract from the same plant showed different activities; the aqueous extract (hot) showed the same or greater activity than the ethanolic extract. by disc diffusion, hot aqueous extract 2000 and 3000 µg/ml inhibition zone was observed against five gram negative pathogens in almost similar pattern . Ethanolic extract was active only against Ps.aeruginosa and K. pneumoniae. The MIC and MBC values of the ethanolic and aqueous extract of Capsella Bursa was active against most bacteria. All isolates were tested by different concentration of sub-MIC of aqueous and ethanolic extracts give different activities of these concentrations to inhibit or omitting the ability of those isolates to produce virulence factors (DNase, Haemolysin production and Lipase production). Finally, the Broth dilution assay showed larger activity than the disc diffusion method.

Published in American Journal of BioScience (Volume 1, Issue 1)
DOI 10.11648/j.ajbio.20130101.12
Page(s) 6-10
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Antibacterial Activity, Capsella Bursa, Bacteria

References
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  • APA Style

    Raad N. Hasan, Mun’im R. Ali, Sinna M. Shakier, Anfal M. Khudhair, Moayad S. Hussin, et al. (2013). Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria. American Journal of BioScience, 1(1), 6-10. https://doi.org/10.11648/j.ajbio.20130101.12

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    ACS Style

    Raad N. Hasan; Mun’im R. Ali; Sinna M. Shakier; Anfal M. Khudhair; Moayad S. Hussin, et al. Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria. Am. J. BioScience 2013, 1(1), 6-10. doi: 10.11648/j.ajbio.20130101.12

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    AMA Style

    Raad N. Hasan, Mun’im R. Ali, Sinna M. Shakier, Anfal M. Khudhair, Moayad S. Hussin, et al. Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria. Am J BioScience. 2013;1(1):6-10. doi: 10.11648/j.ajbio.20130101.12

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  • @article{10.11648/j.ajbio.20130101.12,
      author = {Raad N. Hasan and Mun’im R. Ali and Sinna M. Shakier and Anfal M. Khudhair and Moayad S. Hussin and Yasamin A. Kadum and Ali I. Mohammed and Ahmaed A. Abbas},
      title = {Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria},
      journal = {American Journal of BioScience},
      volume = {1},
      number = {1},
      pages = {6-10},
      doi = {10.11648/j.ajbio.20130101.12},
      url = {https://doi.org/10.11648/j.ajbio.20130101.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130101.12},
      abstract = {Antibacterial activity of ethanolic and aqueous extracts of Capsella Bursa were carried out against eight different species of bacteria, Gram-positive Staphylococcus aureus and Enterococcus fecalis and Gram-negative Escherichia coli, Proteus vulgaris, Serratia marcescens,Acinitobacter bumani, Klebsiella pneumoniae and Pseudomonas aeruginosa. It is active antibacterial only against gram-negative bacteria. The ethanolic and aqueous extract from the same plant showed different activities; the aqueous extract (hot) showed the same or greater activity than the ethanolic extract. by disc diffusion, hot aqueous extract 2000 and 3000 µg/ml inhibition zone was observed against five gram negative pathogens in almost similar pattern . Ethanolic extract was active only against Ps.aeruginosa and K. pneumoniae. The MIC and MBC values of the ethanolic and aqueous extract of Capsella Bursa was active against most bacteria. All isolates were tested by different concentration of sub-MIC of aqueous and ethanolic extracts give different activities of these concentrations to inhibit or omitting the ability of those isolates to produce virulence factors (DNase, Haemolysin production and Lipase production). Finally, the Broth dilution assay showed larger activity than the disc diffusion method.},
     year = {2013}
    }
    

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    T1  - Antibacterial Activity of Aqueous and Alcoholic Extracts of Capsella Bursa against Selected Pathogenic Bacteria
    AU  - Raad N. Hasan
    AU  - Mun’im R. Ali
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    AU  - Ahmaed A. Abbas
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    DO  - 10.11648/j.ajbio.20130101.12
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
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    EP  - 10
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20130101.12
    AB  - Antibacterial activity of ethanolic and aqueous extracts of Capsella Bursa were carried out against eight different species of bacteria, Gram-positive Staphylococcus aureus and Enterococcus fecalis and Gram-negative Escherichia coli, Proteus vulgaris, Serratia marcescens,Acinitobacter bumani, Klebsiella pneumoniae and Pseudomonas aeruginosa. It is active antibacterial only against gram-negative bacteria. The ethanolic and aqueous extract from the same plant showed different activities; the aqueous extract (hot) showed the same or greater activity than the ethanolic extract. by disc diffusion, hot aqueous extract 2000 and 3000 µg/ml inhibition zone was observed against five gram negative pathogens in almost similar pattern . Ethanolic extract was active only against Ps.aeruginosa and K. pneumoniae. The MIC and MBC values of the ethanolic and aqueous extract of Capsella Bursa was active against most bacteria. All isolates were tested by different concentration of sub-MIC of aqueous and ethanolic extracts give different activities of these concentrations to inhibit or omitting the ability of those isolates to produce virulence factors (DNase, Haemolysin production and Lipase production). Finally, the Broth dilution assay showed larger activity than the disc diffusion method.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

  • Ministry of health Teaching laboratory, Baghdad, Iraq

  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

  • Department of biology. Collage of science , University of Al-Mustansiryah, Baghdad, Iraq

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