Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats

Fu Xianzhao; Huang Guangming; Li Chunyan; Huang Wenhua; Qiu Haixian; Qiu Siyuan; Bi Honghan; Cao Qiuxia; Lu Mian

doi:doi:10.11648/j.sjph.20190706.17

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Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats

Fu Xianzhao, Huang Guangming, Li Chunyan, Huang Wenhua, Qiu Haixian, Qiu Siyuan, Bi Honghan, Cao Qiuxia, Lu Mian

Published in Science Journal of Public Health (Volume 7, Issue 6)

Received: 28 October 2019 Published: 7 January 2020

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Abstract

Objective To explore the therapeutic molecular mechanism of Jiang Tang Shu Xin (JTSX) recipe with“invigorating Qi, nourishing Yin, activating blood circulation and detoxifying” in improving insulin resistance, inhibiting apoptotic bypass of endoplasmic reticulum stress (ERS) CCAAT enhance-binding protein homologous protein (CHOP), improving diabetic myocardial remodeling in SD diabetic rats. Methods SD rats were used to establish diabetes mellitus models, and after modeled successfully, were randomly divided into model group, western medicine group (Gliquidone+ Benazepril), low-dose JTSX group (JTSX₁), high-dose JTSX group (JTSX₂), and accepted corresponding drugs for 2 months respectively, taking the same batch of rats as normal control. After drugs administration finishing, blood lipid were measured by automatic biochemical analyzer, fasting serum insulin (FINS) and glycosylated hemoglobin (GHb) were measured by enzyme-linked immunosorbent assay (ELISA), insulin resistance index (IRI) was calculated. Masson staining was used to detect the expression of myocardial collagen fibers, immunohistochemistry to test the expression of myocardial nuclear factor-kB (NF-kB), tumor necrosis factor-alpha (TNF-α), TUNEL to check the apoptotic level of myocardial cells, RT-PCR to detect the transcription level of ERS molecules glucose regulated protein 78 (GRP78) and CHOP. Results Compared with the model group, the treatment groups could significantly reduce TG, LDL-C, GHb and IRI (P<0.05), increase FINS and HDL-C (P<0.05), decrease inflammatory factors NF-kB and TNF-α (P<0.05), down-regulate transcription of CHOP and GRP78 (P<0.05), and reduce cardiomyocyte apoptosis index (AI) (P<0.05); compared with the western medicine group, JTSX2 had more significant effect (P<0.05). Conclusion JTSX can inhibit insulin resistance, correct lipid metabolism disorder, restrain ERS-induced inflammatory reaction, suppress ERS-initiated apoptotic bypass of CHOP, and improve myocardial remodeling, with dose-dependent.

Published in	Science Journal of Public Health (Volume 7, Issue 6)
DOI	10.11648/j.sjph.20190706.17
Page(s)	225-231
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Endoplasmic Reticulum Stress, Apoptosis, CCAAT Enhance-Binding Protein Homologous Protein, Myocardial Remodeling, Jiang Tang Shu Xin Recipe

References

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APA Style

Fu Xianzhao, Huang Guangming, Li Chunyan, Huang Wenhua, Qiu Haixian, et al. (2020). Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats. Science Journal of Public Health, 7(6), 225-231. https://doi.org/10.11648/j.sjph.20190706.17

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ACS Style

Fu Xianzhao; Huang Guangming; Li Chunyan; Huang Wenhua; Qiu Haixian, et al. Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats. Sci. J. Public Health 2020, 7(6), 225-231. doi: 10.11648/j.sjph.20190706.17

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AMA Style

Fu Xianzhao, Huang Guangming, Li Chunyan, Huang Wenhua, Qiu Haixian, et al. Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats. Sci J Public Health. 2020;7(6):225-231. doi: 10.11648/j.sjph.20190706.17

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@article{10.11648/j.sjph.20190706.17,
  author = {Fu Xianzhao and Huang Guangming and Li Chunyan and Huang Wenhua and Qiu Haixian and Qiu Siyuan and Bi Honghan and Cao Qiuxia and Lu Mian},
  title = {Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats},
  journal = {Science Journal of Public Health},
  volume = {7},
  number = {6},
  pages = {225-231},
  doi = {10.11648/j.sjph.20190706.17},
  url = {https://doi.org/10.11648/j.sjph.20190706.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20190706.17},
  abstract = {Objective To explore the therapeutic molecular mechanism of Jiang Tang Shu Xin (JTSX) recipe with“invigorating Qi, nourishing Yin, activating blood circulation and detoxifying” in improving insulin resistance, inhibiting apoptotic bypass of endoplasmic reticulum stress (ERS) CCAAT enhance-binding protein homologous protein (CHOP), improving diabetic myocardial remodeling in SD diabetic rats. Methods SD rats were used to establish diabetes mellitus models, and after modeled successfully, were randomly divided into model group, western medicine group (Gliquidone+ Benazepril), low-dose JTSX group (JTSX1), high-dose JTSX group (JTSX2), and accepted corresponding drugs for 2 months respectively, taking the same batch of rats as normal control. After drugs administration finishing, blood lipid were measured by automatic biochemical analyzer, fasting serum insulin (FINS) and glycosylated hemoglobin (GHb) were measured by enzyme-linked immunosorbent assay (ELISA), insulin resistance index (IRI) was calculated. Masson staining was used to detect the expression of myocardial collagen fibers, immunohistochemistry to test the expression of myocardial nuclear factor-kB (NF-kB), tumor necrosis factor-alpha (TNF-α), TUNEL to check the apoptotic level of myocardial cells, RT-PCR to detect the transcription level of ERS molecules glucose regulated protein 78 (GRP78) and CHOP. Results Compared with the model group, the treatment groups could significantly reduce TG, LDL-C, GHb and IRI (PConclusion JTSX can inhibit insulin resistance, correct lipid metabolism disorder, restrain ERS-induced inflammatory reaction, suppress ERS-initiated apoptotic bypass of CHOP, and improve myocardial remodeling, with dose-dependent.},
 year = {2020}
}

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TY  - JOUR
T1  - Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats
AU  - Fu Xianzhao
AU  - Huang Guangming
AU  - Li Chunyan
AU  - Huang Wenhua
AU  - Qiu Haixian
AU  - Qiu Siyuan
AU  - Bi Honghan
AU  - Cao Qiuxia
AU  - Lu Mian
Y1  - 2020/01/07
PY  - 2020
N1  - https://doi.org/10.11648/j.sjph.20190706.17
DO  - 10.11648/j.sjph.20190706.17
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 225
EP  - 231
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20190706.17
AB  - Objective To explore the therapeutic molecular mechanism of Jiang Tang Shu Xin (JTSX) recipe with“invigorating Qi, nourishing Yin, activating blood circulation and detoxifying” in improving insulin resistance, inhibiting apoptotic bypass of endoplasmic reticulum stress (ERS) CCAAT enhance-binding protein homologous protein (CHOP), improving diabetic myocardial remodeling in SD diabetic rats. Methods SD rats were used to establish diabetes mellitus models, and after modeled successfully, were randomly divided into model group, western medicine group (Gliquidone+ Benazepril), low-dose JTSX group (JTSX1), high-dose JTSX group (JTSX2), and accepted corresponding drugs for 2 months respectively, taking the same batch of rats as normal control. After drugs administration finishing, blood lipid were measured by automatic biochemical analyzer, fasting serum insulin (FINS) and glycosylated hemoglobin (GHb) were measured by enzyme-linked immunosorbent assay (ELISA), insulin resistance index (IRI) was calculated. Masson staining was used to detect the expression of myocardial collagen fibers, immunohistochemistry to test the expression of myocardial nuclear factor-kB (NF-kB), tumor necrosis factor-alpha (TNF-α), TUNEL to check the apoptotic level of myocardial cells, RT-PCR to detect the transcription level of ERS molecules glucose regulated protein 78 (GRP78) and CHOP. Results Compared with the model group, the treatment groups could significantly reduce TG, LDL-C, GHb and IRI (PConclusion JTSX can inhibit insulin resistance, correct lipid metabolism disorder, restrain ERS-induced inflammatory reaction, suppress ERS-initiated apoptotic bypass of CHOP, and improve myocardial remodeling, with dose-dependent.
VL  - 7
IS  - 6
ER  -

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Author Information

Fu Xianzhao

Clinic Medical College, Youjiang Medical National College, Baise, China
Huang Guangming

Clinic Medical College, Youjiang Medical National College, Baise, China
Li Chunyan

Clinic Medical College, Youjiang Medical National College, Baise, China
Huang Wenhua

Clinic Medical College, Youjiang Medical National College, Baise, China
Qiu Haixian

Clinic Medical College, Youjiang Medical National College, Baise, China
Qiu Siyuan

Clinic Medical College, Youjiang Medical National College, Baise, China
Bi Honghan

Clinic Medical College, Youjiang Medical National College, Baise, China
Cao Qiuxia

Clinic Medical College, Youjiang Medical National College, Baise, China
Lu Mian

Clinic Medical College, Youjiang Medical National College, Baise, China

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APA Style

Fu Xianzhao, Huang Guangming, Li Chunyan, Huang Wenhua, Qiu Haixian, et al. (2020). Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats. Science Journal of Public Health, 7(6), 225-231. https://doi.org/10.11648/j.sjph.20190706.17

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ACS Style

Fu Xianzhao; Huang Guangming; Li Chunyan; Huang Wenhua; Qiu Haixian, et al. Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats. Sci. J. Public Health 2020, 7(6), 225-231. doi: 10.11648/j.sjph.20190706.17

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AMA Style

Fu Xianzhao, Huang Guangming, Li Chunyan, Huang Wenhua, Qiu Haixian, et al. Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats. Sci J Public Health. 2020;7(6):225-231. doi: 10.11648/j.sjph.20190706.17

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@article{10.11648/j.sjph.20190706.17,
  author = {Fu Xianzhao and Huang Guangming and Li Chunyan and Huang Wenhua and Qiu Haixian and Qiu Siyuan and Bi Honghan and Cao Qiuxia and Lu Mian},
  title = {Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats},
  journal = {Science Journal of Public Health},
  volume = {7},
  number = {6},
  pages = {225-231},
  doi = {10.11648/j.sjph.20190706.17},
  url = {https://doi.org/10.11648/j.sjph.20190706.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20190706.17},
  abstract = {Objective To explore the therapeutic molecular mechanism of Jiang Tang Shu Xin (JTSX) recipe with“invigorating Qi, nourishing Yin, activating blood circulation and detoxifying” in improving insulin resistance, inhibiting apoptotic bypass of endoplasmic reticulum stress (ERS) CCAAT enhance-binding protein homologous protein (CHOP), improving diabetic myocardial remodeling in SD diabetic rats. Methods SD rats were used to establish diabetes mellitus models, and after modeled successfully, were randomly divided into model group, western medicine group (Gliquidone+ Benazepril), low-dose JTSX group (JTSX1), high-dose JTSX group (JTSX2), and accepted corresponding drugs for 2 months respectively, taking the same batch of rats as normal control. After drugs administration finishing, blood lipid were measured by automatic biochemical analyzer, fasting serum insulin (FINS) and glycosylated hemoglobin (GHb) were measured by enzyme-linked immunosorbent assay (ELISA), insulin resistance index (IRI) was calculated. Masson staining was used to detect the expression of myocardial collagen fibers, immunohistochemistry to test the expression of myocardial nuclear factor-kB (NF-kB), tumor necrosis factor-alpha (TNF-α), TUNEL to check the apoptotic level of myocardial cells, RT-PCR to detect the transcription level of ERS molecules glucose regulated protein 78 (GRP78) and CHOP. Results Compared with the model group, the treatment groups could significantly reduce TG, LDL-C, GHb and IRI (PConclusion JTSX can inhibit insulin resistance, correct lipid metabolism disorder, restrain ERS-induced inflammatory reaction, suppress ERS-initiated apoptotic bypass of CHOP, and improve myocardial remodeling, with dose-dependent.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Inhibition of Jiang Tang Shu Xin (JTSX) Recipe in Endoplasmic Reticulum Stress CHOP Pathway and Improving Myocardial Remodeling in SD Diabetic Rats
AU  - Fu Xianzhao
AU  - Huang Guangming
AU  - Li Chunyan
AU  - Huang Wenhua
AU  - Qiu Haixian
AU  - Qiu Siyuan
AU  - Bi Honghan
AU  - Cao Qiuxia
AU  - Lu Mian
Y1  - 2020/01/07
PY  - 2020
N1  - https://doi.org/10.11648/j.sjph.20190706.17
DO  - 10.11648/j.sjph.20190706.17
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 225
EP  - 231
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20190706.17
AB  - Objective To explore the therapeutic molecular mechanism of Jiang Tang Shu Xin (JTSX) recipe with“invigorating Qi, nourishing Yin, activating blood circulation and detoxifying” in improving insulin resistance, inhibiting apoptotic bypass of endoplasmic reticulum stress (ERS) CCAAT enhance-binding protein homologous protein (CHOP), improving diabetic myocardial remodeling in SD diabetic rats. Methods SD rats were used to establish diabetes mellitus models, and after modeled successfully, were randomly divided into model group, western medicine group (Gliquidone+ Benazepril), low-dose JTSX group (JTSX1), high-dose JTSX group (JTSX2), and accepted corresponding drugs for 2 months respectively, taking the same batch of rats as normal control. After drugs administration finishing, blood lipid were measured by automatic biochemical analyzer, fasting serum insulin (FINS) and glycosylated hemoglobin (GHb) were measured by enzyme-linked immunosorbent assay (ELISA), insulin resistance index (IRI) was calculated. Masson staining was used to detect the expression of myocardial collagen fibers, immunohistochemistry to test the expression of myocardial nuclear factor-kB (NF-kB), tumor necrosis factor-alpha (TNF-α), TUNEL to check the apoptotic level of myocardial cells, RT-PCR to detect the transcription level of ERS molecules glucose regulated protein 78 (GRP78) and CHOP. Results Compared with the model group, the treatment groups could significantly reduce TG, LDL-C, GHb and IRI (PConclusion JTSX can inhibit insulin resistance, correct lipid metabolism disorder, restrain ERS-induced inflammatory reaction, suppress ERS-initiated apoptotic bypass of CHOP, and improve myocardial remodeling, with dose-dependent.
VL  - 7
IS  - 6
ER  -

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