Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats

Udokang Nsikak Ephraim; Udom Utibe Godwin

doi:doi:10.11648/j.sjph.20190705.12

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Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats

Udokang Nsikak Ephraim, Udom Utibe Godwin

Published in Science Journal of Public Health (Volume 7, Issue 5)

Received: 20 May 2019 Accepted: 26 June 2019 Published: 25 September 2019

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Abstract

This study was carried out with a total of forty (40) male wistar rats randomly divided into four groups of ten (10) rats per group. Group 1 was the control and was treated with distilled water, group 2 was the low dose group (exposed to marijuana smoke for 5 minutes daily), group 3 was the high dose group (exposed to marijuana smoke for 10 minutes daily) and group 4 was the high dose + vitamin C group (exposed to marijuana smoke for 10 minutes and orally gavaged with vitamin C at 2.8 mg/kg body weight daily). The animals were sacrificed on the 29th day and testes were collected. Semen sample were collected and analyzed of Total sperm cell in sample (106/ml), Sperm Concentration (106/ml), Number of Motile sperm, Progressivity, Velocity of Active Path (µm/s). Testicular histology was carried out. The result showed a dose-dependent decrease in all the measured parameters in groups treated with marijuana (group 2 and 3) and an almost equivalent value with the control group for the group treated with marijuana + vitamin C (group 4). The testicular histological section reveals hypochromic section seminiferous tubules with swollen germinal cells and spermatogenic lining cell and irregular alignment of myoid cells for the group 2 and 3 when compared with the control group. But these damages were observed to be ameliorated in the marijuana + vitamin C group (group 4). From this result, it can therefore be concluded that marijuana possesses an antifertility property as seen in the decrease in total sperm count, Sperm Concentration, Number of Motile sperm, Progressivity, Velocity of Active Path and deformation of the testes of rats in this study. Also that vitamin C can ameliorate the antifertility effects of inhaled marijuana in rats.

Published in	Science Journal of Public Health (Volume 7, Issue 5)
DOI	10.11648/j.sjph.20190705.12
Page(s)	140-150
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Marijuana, Vitamin C, Semen Analysis, Testicular Histology, Reactive Oxygen Specie (ROS), Antioxidant, Oxidative Stress, Antifertility

References

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APA Style

Udokang Nsikak Ephraim, Udom Utibe Godwin. (2019). Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats. Science Journal of Public Health, 7(5), 140-150. https://doi.org/10.11648/j.sjph.20190705.12

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ACS Style

Udokang Nsikak Ephraim; Udom Utibe Godwin. Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats. Sci. J. Public Health 2019, 7(5), 140-150. doi: 10.11648/j.sjph.20190705.12

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AMA Style

Udokang Nsikak Ephraim, Udom Utibe Godwin. Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats. Sci J Public Health. 2019;7(5):140-150. doi: 10.11648/j.sjph.20190705.12

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@article{10.11648/j.sjph.20190705.12,
  author = {Udokang Nsikak Ephraim and Udom Utibe Godwin},
  title = {Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats},
  journal = {Science Journal of Public Health},
  volume = {7},
  number = {5},
  pages = {140-150},
  doi = {10.11648/j.sjph.20190705.12},
  url = {https://doi.org/10.11648/j.sjph.20190705.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20190705.12},
  abstract = {This study was carried out with a total of forty (40) male wistar rats randomly divided into four groups of ten (10) rats per group. Group 1 was the control and was treated with distilled water, group 2 was the low dose group (exposed to marijuana smoke for 5 minutes daily), group 3 was the high dose group (exposed to marijuana smoke for 10 minutes daily) and group 4 was the high dose + vitamin C group (exposed to marijuana smoke for 10 minutes and orally gavaged with vitamin C at 2.8 mg/kg body weight daily). The animals were sacrificed on the 29th day and testes were collected. Semen sample were collected and analyzed of Total sperm cell in sample (106/ml), Sperm Concentration (106/ml), Number of Motile sperm, Progressivity, Velocity of Active Path (µm/s). Testicular histology was carried out. The result showed a dose-dependent decrease in all the measured parameters in groups treated with marijuana (group 2 and 3) and an almost equivalent value with the control group for the group treated with marijuana + vitamin C (group 4). The testicular histological section reveals hypochromic section seminiferous tubules with swollen germinal cells and spermatogenic lining cell and irregular alignment of myoid cells for the group 2 and 3 when compared with the control group. But these damages were observed to be ameliorated in the marijuana + vitamin C group (group 4). From this result, it can therefore be concluded that marijuana possesses an antifertility property as seen in the decrease in total sperm count, Sperm Concentration, Number of Motile sperm, Progressivity, Velocity of Active Path and deformation of the testes of rats in this study. Also that vitamin C can ameliorate the antifertility effects of inhaled marijuana in rats.},
 year = {2019}
}

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TY  - JOUR
T1  - Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats
AU  - Udokang Nsikak Ephraim
AU  - Udom Utibe Godwin
Y1  - 2019/09/25
PY  - 2019
N1  - https://doi.org/10.11648/j.sjph.20190705.12
DO  - 10.11648/j.sjph.20190705.12
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 140
EP  - 150
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20190705.12
AB  - This study was carried out with a total of forty (40) male wistar rats randomly divided into four groups of ten (10) rats per group. Group 1 was the control and was treated with distilled water, group 2 was the low dose group (exposed to marijuana smoke for 5 minutes daily), group 3 was the high dose group (exposed to marijuana smoke for 10 minutes daily) and group 4 was the high dose + vitamin C group (exposed to marijuana smoke for 10 minutes and orally gavaged with vitamin C at 2.8 mg/kg body weight daily). The animals were sacrificed on the 29th day and testes were collected. Semen sample were collected and analyzed of Total sperm cell in sample (106/ml), Sperm Concentration (106/ml), Number of Motile sperm, Progressivity, Velocity of Active Path (µm/s). Testicular histology was carried out. The result showed a dose-dependent decrease in all the measured parameters in groups treated with marijuana (group 2 and 3) and an almost equivalent value with the control group for the group treated with marijuana + vitamin C (group 4). The testicular histological section reveals hypochromic section seminiferous tubules with swollen germinal cells and spermatogenic lining cell and irregular alignment of myoid cells for the group 2 and 3 when compared with the control group. But these damages were observed to be ameliorated in the marijuana + vitamin C group (group 4). From this result, it can therefore be concluded that marijuana possesses an antifertility property as seen in the decrease in total sperm count, Sperm Concentration, Number of Motile sperm, Progressivity, Velocity of Active Path and deformation of the testes of rats in this study. Also that vitamin C can ameliorate the antifertility effects of inhaled marijuana in rats.
VL  - 7
IS  - 5
ER  -

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Author Information

Udokang Nsikak Ephraim

Department of Physiology, University of Uyo, Uyo, Nigeria
Udom Utibe Godwin

Department of Physiology, University of Uyo, Uyo, Nigeria

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Udokang Nsikak Ephraim, Udom Utibe Godwin. (2019). Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats. Science Journal of Public Health, 7(5), 140-150. https://doi.org/10.11648/j.sjph.20190705.12

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ACS Style

Udokang Nsikak Ephraim; Udom Utibe Godwin. Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats. Sci. J. Public Health 2019, 7(5), 140-150. doi: 10.11648/j.sjph.20190705.12

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AMA Style

Udokang Nsikak Ephraim, Udom Utibe Godwin. Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats. Sci J Public Health. 2019;7(5):140-150. doi: 10.11648/j.sjph.20190705.12

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@article{10.11648/j.sjph.20190705.12,
  author = {Udokang Nsikak Ephraim and Udom Utibe Godwin},
  title = {Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats},
  journal = {Science Journal of Public Health},
  volume = {7},
  number = {5},
  pages = {140-150},
  doi = {10.11648/j.sjph.20190705.12},
  url = {https://doi.org/10.11648/j.sjph.20190705.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20190705.12},
  abstract = {This study was carried out with a total of forty (40) male wistar rats randomly divided into four groups of ten (10) rats per group. Group 1 was the control and was treated with distilled water, group 2 was the low dose group (exposed to marijuana smoke for 5 minutes daily), group 3 was the high dose group (exposed to marijuana smoke for 10 minutes daily) and group 4 was the high dose + vitamin C group (exposed to marijuana smoke for 10 minutes and orally gavaged with vitamin C at 2.8 mg/kg body weight daily). The animals were sacrificed on the 29th day and testes were collected. Semen sample were collected and analyzed of Total sperm cell in sample (106/ml), Sperm Concentration (106/ml), Number of Motile sperm, Progressivity, Velocity of Active Path (µm/s). Testicular histology was carried out. The result showed a dose-dependent decrease in all the measured parameters in groups treated with marijuana (group 2 and 3) and an almost equivalent value with the control group for the group treated with marijuana + vitamin C (group 4). The testicular histological section reveals hypochromic section seminiferous tubules with swollen germinal cells and spermatogenic lining cell and irregular alignment of myoid cells for the group 2 and 3 when compared with the control group. But these damages were observed to be ameliorated in the marijuana + vitamin C group (group 4). From this result, it can therefore be concluded that marijuana possesses an antifertility property as seen in the decrease in total sperm count, Sperm Concentration, Number of Motile sperm, Progressivity, Velocity of Active Path and deformation of the testes of rats in this study. Also that vitamin C can ameliorate the antifertility effects of inhaled marijuana in rats.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Vitamin C Ameliorates the Antifertility Effects of Inhaled Marijuana in Rats
AU  - Udokang Nsikak Ephraim
AU  - Udom Utibe Godwin
Y1  - 2019/09/25
PY  - 2019
N1  - https://doi.org/10.11648/j.sjph.20190705.12
DO  - 10.11648/j.sjph.20190705.12
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 140
EP  - 150
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20190705.12
AB  - This study was carried out with a total of forty (40) male wistar rats randomly divided into four groups of ten (10) rats per group. Group 1 was the control and was treated with distilled water, group 2 was the low dose group (exposed to marijuana smoke for 5 minutes daily), group 3 was the high dose group (exposed to marijuana smoke for 10 minutes daily) and group 4 was the high dose + vitamin C group (exposed to marijuana smoke for 10 minutes and orally gavaged with vitamin C at 2.8 mg/kg body weight daily). The animals were sacrificed on the 29th day and testes were collected. Semen sample were collected and analyzed of Total sperm cell in sample (106/ml), Sperm Concentration (106/ml), Number of Motile sperm, Progressivity, Velocity of Active Path (µm/s). Testicular histology was carried out. The result showed a dose-dependent decrease in all the measured parameters in groups treated with marijuana (group 2 and 3) and an almost equivalent value with the control group for the group treated with marijuana + vitamin C (group 4). The testicular histological section reveals hypochromic section seminiferous tubules with swollen germinal cells and spermatogenic lining cell and irregular alignment of myoid cells for the group 2 and 3 when compared with the control group. But these damages were observed to be ameliorated in the marijuana + vitamin C group (group 4). From this result, it can therefore be concluded that marijuana possesses an antifertility property as seen in the decrease in total sperm count, Sperm Concentration, Number of Motile sperm, Progressivity, Velocity of Active Path and deformation of the testes of rats in this study. Also that vitamin C can ameliorate the antifertility effects of inhaled marijuana in rats.
VL  - 7
IS  - 5
ER  -

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