Background: Rotavirus is the most common cause of severe diarrhoea among children < 5 years of age worldwide. To determine the prevalence of severe rotavirus infection in children admitted with acute gastroenteritis attending Mbabane Government Referral Hospital in the Hhohho region and Raleigh Fitkin Memorial (RFM) Hospital in the Manzini Region of Swaziland, sentinel surveillance was conducted in January 2013 – December 2014. Methods: This is hospital based sentinel surveillance study. A total of 331 children under the age of five years with acute diarrhoea that were admitted to the hospitals were enrolled into the sentinel surveillance and 302 cases had stool samples tested for rotavirus antigens by enzyme immunoassay ProSpec T Rotavirus kit. The G and P genotypes were established by multiplex nested reverse transcription polymerase chain reaction. Results: Rotavirus was detected in 159 (52.6%) of the 302 children with acute diarrhoea that had stool specimens collected and 108 (68%) of positive cases of rotavirus gastroenteritis were between 6-11 months of age and 91% were below the age of two years. Rotavirus infection occurred with peaks between June - August. During the 2013 – 2014 period, 146 samples were subjected for reverse transcription polymerase chain reaction and genotyping assays. The most common genotypes detected in Swaziland were 82% G2P [4] in 2013 and 93% G1P [8] in 2014. Conclusion: Swaziland would benefit by introducing rotavirus vaccine and hence reduce the hospitalization burden of managing acute diarrhoea cases attributed to Rotavirus. The data from the sentinel hospitals can also be used to monitor the effectiveness of the vaccine once introduced. 1. National Reference Laboratory, Ministry of health, Swaziland- MOH 2. World Health Organization, Inter country support team for East and Southern Africa, Zimbabwe, Harare, 3. Ministry of health, Swaziland 4. World Health Organization, Swaziland 5. Mbabane Government Hospital, Swaziland 6. Raleigh Fitkin Memorial Hospital, Swaziland
| Published in | Science Journal of Public Health (Volume 5, Issue 4) |
| DOI | 10.11648/j.sjph.20170504.22 |
| Page(s) | 353-358 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Diarrhoea, Rotavirus, Prevalence, Genotypes, Swaziland
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APA Style
Gugu Maphalala, Goitom Weldegebriel, Nomcebo Phungwayo, Eunice Ruhinda, Njabulo Lukhele, et al. (2017). Prevalence of Rotavirus in Under 5 Year Old Children Hospitalized for Acute Gastroenteritis, Swaziland, 2013 - 2014. Science Journal of Public Health, 5(4), 353-358. https://doi.org/10.11648/j.sjph.20170504.22
ACS Style
Gugu Maphalala; Goitom Weldegebriel; Nomcebo Phungwayo; Eunice Ruhinda; Njabulo Lukhele, et al. Prevalence of Rotavirus in Under 5 Year Old Children Hospitalized for Acute Gastroenteritis, Swaziland, 2013 - 2014. Sci. J. Public Health 2017, 5(4), 353-358. doi: 10.11648/j.sjph.20170504.22
AMA Style
Gugu Maphalala, Goitom Weldegebriel, Nomcebo Phungwayo, Eunice Ruhinda, Njabulo Lukhele, et al. Prevalence of Rotavirus in Under 5 Year Old Children Hospitalized for Acute Gastroenteritis, Swaziland, 2013 - 2014. Sci J Public Health. 2017;5(4):353-358. doi: 10.11648/j.sjph.20170504.22
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author = {Gugu Maphalala and Goitom Weldegebriel and Nomcebo Phungwayo and Eunice Ruhinda and Njabulo Lukhele and Getahun Tsegaye and Gilbert Masona and Nomsa Nomcebo Dube and Nonhlanhla Dlamini and Angel Dlamini and Philile Shabangu and Lonkululeko Khumalo},
title = {Prevalence of Rotavirus in Under 5 Year Old Children Hospitalized for Acute Gastroenteritis, Swaziland, 2013 - 2014},
journal = {Science Journal of Public Health},
volume = {5},
number = {4},
pages = {353-358},
doi = {10.11648/j.sjph.20170504.22},
url = {https://doi.org/10.11648/j.sjph.20170504.22},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20170504.22},
abstract = {Background: Rotavirus is the most common cause of severe diarrhoea among children Methods: This is hospital based sentinel surveillance study. A total of 331 children under the age of five years with acute diarrhoea that were admitted to the hospitals were enrolled into the sentinel surveillance and 302 cases had stool samples tested for rotavirus antigens by enzyme immunoassay ProSpec T Rotavirus kit. The G and P genotypes were established by multiplex nested reverse transcription polymerase chain reaction. Results: Rotavirus was detected in 159 (52.6%) of the 302 children with acute diarrhoea that had stool specimens collected and 108 (68%) of positive cases of rotavirus gastroenteritis were between 6-11 months of age and 91% were below the age of two years. Rotavirus infection occurred with peaks between June - August. During the 2013 – 2014 period, 146 samples were subjected for reverse transcription polymerase chain reaction and genotyping assays. The most common genotypes detected in Swaziland were 82% G2P [4] in 2013 and 93% G1P [8] in 2014. Conclusion: Swaziland would benefit by introducing rotavirus vaccine and hence reduce the hospitalization burden of managing acute diarrhoea cases attributed to Rotavirus. The data from the sentinel hospitals can also be used to monitor the effectiveness of the vaccine once introduced. 1. National Reference Laboratory, Ministry of health, Swaziland- MOH 2. World Health Organization, Inter country support team for East and Southern Africa, Zimbabwe, Harare, 3. Ministry of health, Swaziland 4. World Health Organization, Swaziland 5. Mbabane Government Hospital, Swaziland 6. Raleigh Fitkin Memorial Hospital, Swaziland},
year = {2017}
}
TY - JOUR T1 - Prevalence of Rotavirus in Under 5 Year Old Children Hospitalized for Acute Gastroenteritis, Swaziland, 2013 - 2014 AU - Gugu Maphalala AU - Goitom Weldegebriel AU - Nomcebo Phungwayo AU - Eunice Ruhinda AU - Njabulo Lukhele AU - Getahun Tsegaye AU - Gilbert Masona AU - Nomsa Nomcebo Dube AU - Nonhlanhla Dlamini AU - Angel Dlamini AU - Philile Shabangu AU - Lonkululeko Khumalo Y1 - 2017/07/26 PY - 2017 N1 - https://doi.org/10.11648/j.sjph.20170504.22 DO - 10.11648/j.sjph.20170504.22 T2 - Science Journal of Public Health JF - Science Journal of Public Health JO - Science Journal of Public Health SP - 353 EP - 358 PB - Science Publishing Group SN - 2328-7950 UR - https://doi.org/10.11648/j.sjph.20170504.22 AB - Background: Rotavirus is the most common cause of severe diarrhoea among children Methods: This is hospital based sentinel surveillance study. A total of 331 children under the age of five years with acute diarrhoea that were admitted to the hospitals were enrolled into the sentinel surveillance and 302 cases had stool samples tested for rotavirus antigens by enzyme immunoassay ProSpec T Rotavirus kit. The G and P genotypes were established by multiplex nested reverse transcription polymerase chain reaction. Results: Rotavirus was detected in 159 (52.6%) of the 302 children with acute diarrhoea that had stool specimens collected and 108 (68%) of positive cases of rotavirus gastroenteritis were between 6-11 months of age and 91% were below the age of two years. Rotavirus infection occurred with peaks between June - August. During the 2013 – 2014 period, 146 samples were subjected for reverse transcription polymerase chain reaction and genotyping assays. The most common genotypes detected in Swaziland were 82% G2P [4] in 2013 and 93% G1P [8] in 2014. Conclusion: Swaziland would benefit by introducing rotavirus vaccine and hence reduce the hospitalization burden of managing acute diarrhoea cases attributed to Rotavirus. The data from the sentinel hospitals can also be used to monitor the effectiveness of the vaccine once introduced. 1. National Reference Laboratory, Ministry of health, Swaziland- MOH 2. World Health Organization, Inter country support team for East and Southern Africa, Zimbabwe, Harare, 3. Ministry of health, Swaziland 4. World Health Organization, Swaziland 5. Mbabane Government Hospital, Swaziland 6. Raleigh Fitkin Memorial Hospital, Swaziland VL - 5 IS - 4 ER -
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