Antioxidant Screening of Various Solvent Extracts of <i>Cuminum cyminum </i>Cultivated in Bangladesh

Fardushi Rahman; Sabina Easmin; Shahed Zaman; Hamidul Haq; Rezaul Haque Ansary; Subed Chandra Dev Sharma; Suganya Murugesu; Vikneswari Perumal

doi:doi:10.11648/j.sjc.20210902.13

Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh

Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, Subed Chandra Dev Sharma, Suganya Murugesu, Vikneswari Perumal

Published in Science Journal of Chemistry (Volume 9, Issue 2)

Received: 11 March 2021 Accepted: 2 April 2021 Published: 23 April 2021

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Abstract

Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC₅₀ (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.

Published in	Science Journal of Chemistry (Volume 9, Issue 2)
DOI	10.11648/j.sjc.20210902.13
Page(s)	54-61
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Solvents, Extraction Yield, Antioxidant Activity, Cuminum cyminum, Bangladesh

References

[1]	Atanasov, S., Stoylov, B. L., Saykova, I., & Tchaoushev (2019). Mass transfer intensification in bioactive compounds recovery by alternative extraction methods: effects of solvent. Global NEST Journal, 21 (1): 30-36.
[2]	Markom, M., Hasan, M., Daud, W., Singh, H., & Jahim, M. (2007). Extraction of hydrolyzable tannins from Phyllanthusniruri Linn. Effects of solvents and extraction methods. Seperation and Purification Technology, 52: 487-496.
[3]	Sultana, B., Anwar, F., & Ashaf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14: 2167-2180.
[4]	Trabelsi, N., Megdiche, W., Ksouri, R., Falleh, H., Oueslati, S., Soumaya, B., Hajlaoui, H., & Abdelly, C. (2010). Solvent effects on phenolic contents and biological activities of the halophyte Limoniastrummonopetalum leaves. LWT-Food Science Technology, 43: 632-639.
[5]	Dua, A., Gupta, K., Mittal, A., & Mahajan, R. (2012). A study of antioxidant properties and antioxidant compounds of Cumin (Cuminum cyminum). International Journal of Pharmaceutical and Biological Archives, 3: 1110-1116.
[6]	Chan, E. W. C., Lim., Y. Y., Wong, S. K., Lim., K. K., Tan, S. P., Lianto, F. S., & Yong, M. Y. (2009). Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113: 166-172.
[7]	Mediana, A., Abas, F., Khatib, A., Maulidiani, H., Shaari, K., Choi, H., & Lajis, H. (2012). 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus. Food Research International, 49: 763-770.
[8]	Esmail, A. (2016). The pharmacological activities of Cuminum cyminum- A review. IOSR Journal of Pharmacy, 6: 46-65.
[9]	Javadi, N., Abas, F., Hamid, A., Simoh, S., Shaari, K., Ismail, S., Mediani, A., & Khatib, A. (2014). GC-MS based metabolite proﬁling of Cosmos caudatus leaves possessing α-glucosidase inhibitory activity. Journal of Food Science, 79: 6.
[10]	Yuliana, D., Khatib, A., Verpoorte, R., & Choi, H. (2011). Comprehensive extraction method integrated with NMR metabolomics: a new bioactivity screeningmethod for plants, adenosine A1 receptor binding compounds in Orthosiphonstamineus Benth. Analytical Chemistry, 83: 6902-6.
[11]	Shirwaikar, A., Shirwaikar, A., Rajendran, K., Punitha, I. S. (2006). In Vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberine. Biological and Pharmaceutical Bulletin, 29: 1906-1910.
[12]	Kumar, R. S., Rajkapoor, B., & Perumal, P. (2013). Anti-inflammatory and anti-nociceptive activities of methanolic leaf extract of Indigo fera cassioides Rottl. Ex. DC. Journal of Acute Disease, 2 (4): 322-326.
[13]	Chandrashekar, K., Santanu, S., & Prasanna, K. (2010). Phytochemical studies of aerialparts of the plant Leucaslavandulae folia. Der Pharma Chemica, 2: 434-437.
[14]	Priyadarsini, A., Chakrapani, S., Tirupathi, N., Shamshad, S., & Basha, M. (2017). Phytochemical analysis of Oroxylum indicum leaf along with screening for antimicrobial and antioxidant properties. Indian Journal of Pharmaceutical Science & Research, 5: 53-70.
[15]	Robinson, R., Gheneim, R., Kozak, A., Ellis, D., & Mansfield, D. (2005). The potential of metabolite profiling as a selection tool for genotype discrimination in Populus. Journal of Experimental Botany, 56: 2807-19.
[16]	Canini, A., Alesiani, D., D'arcangelo, G., & Tagliatesta, P. (2007). Gas chromatography-mass spectrometry analysis of phenolic compounds from Carica papaya L. leaf. Journal of Food Component Analysis, 20: 584-90.
[17]	Sabina, E., Zaidul, M., Ghafoor, K., Jaffri, M., Sahena, F., Babiker, E., Perumal, V., Hamed, M., Amid, M., & Khatib, A. (2015). Screening of various parts of Phaleria macrocarpa plant for α-Glucosidase inhibitory activity. Journal of Food Biochemistry, doi: 10.1111/jfbc 12212, 1745-4514.
[18]	Tunna, S., Zidul, M., Ahmed, U., Al-Juhaimi, Y., Uddin, S., Hasan, M., & Ferdous, S. (2015). Analysis and profiling of extract and fractions of neglected weed Mimosa pudicaLinn. traditionally used in southeast asia to treat diabetes. South African Journal of Botany, 99: 144-152.
[19]	Sugiwati, S., Setiasih, S., & Afifa, E. (2009). Antihyperglycaemic activity of the mahkota Dewa [Phaleria macrocarpa (scheff.) boerl.] leaf extracts as an alpha-glucosidase inhibitor. Makara Journal of Health Research, 13: 74–78.
[20]	Rabyah, A., Atangwho, J., Kuar, N., Ahmad, M., Mahmud, R., & Asmawi, Z. (2013). In vitro and in vivo effects of standardized extract and fractions of Phaleria macrocarpa fruits pericarp on lead carbohydrate digesting enzymes. BMC Complementary and Alternative Medicine, 13: 39.
[21]	Xu, B. J., & Chang, S. K. C. (2007). A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. Journal of Food Science, 72: 159-166.
[22]	Jaiswal, K., Rajauria, G., Abu-Ghannam, N., & Gupta, S. (2012). Effect of different solvents on polyphenolic content, antioxidant capacity and antibacterial activity of IrishYork cabbage. Journal of Food Biochemistry, 36: 344-358.
[23]	Razali, N., Mat-Junti, S., Muthalib, A. F., Subramaniam, S., & Aziz, A. (2012). Effects of 8 various solvents on the extraction of antioxidant phenolics from the leaves, seeds, veins and skins of Tamarindus indica L. Food Chemistry, 131: 441-448.
[24]	Lim, K. J. A., Cabajar, A. A., Lobarbio, C. F. Y., Taboada, E. B., & Lacks, D. J. (2019). Extraction of bioactive compounds from (Mangifera indica L. var. Carabao) seed kernel with ethanol-water binary solvent systems. Journal of Food Science and Technology, 56 (5): 2536-2544.
[25]	Kulkula, W., Aligiannis, N., Halabalaki, M., Skaltsounis, L., Glowniak, K., & Kalpoutzakis, E. (2013). Influence of extraction procedures on phenolic content and antioxidant activity of Cretan barberry herb. Food Chemistry, 138: 406-413.
[26]	Jabin, T., Ahmed, T., Alam, S., Shohael, A. M. (2020). Investigation on phytochemical screening, thin layer chromatographic profiling and antibacterial potential of ten widely used spices in Bangladesh. International Journal of Biosciences, 16 (3): 208-220.
[27]	Hashemian, N., Abdollah, G. P., Masoud, H., Ahmadreza, G., & Behzad, H. (2013). Diversity in chemical composition and antibacterial activity of essential oils of cumin (CuminumcyminumL.) diverse from northeast of Iran. Australian Journal of Crop Science, 7 (11): 1752.
[28]	Mohammadpour, H., Moghimipour, E., Rasooli, I., Fakoor, H., Astaneh, A., Moosaie, S., &Jalili, Z. (2012). Chemical composition and antifungal activity of Cuminum cyminum L. essential oil from Alborz Mountain against Aspergillus species. Jundishapur Journal of Natural Pharmaceutical Products, 7 (2): 50-55.

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Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, et al. (2021). Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Science Journal of Chemistry, 9(2), 54-61. https://doi.org/10.11648/j.sjc.20210902.13

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ACS Style

Fardushi Rahman; Sabina Easmin; Shahed Zaman; Hamidul Haq; Rezaul Haque Ansary, et al. Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Sci. J. Chem. 2021, 9(2), 54-61. doi: 10.11648/j.sjc.20210902.13

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AMA Style

Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, et al. Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Sci J Chem. 2021;9(2):54-61. doi: 10.11648/j.sjc.20210902.13

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@article{10.11648/j.sjc.20210902.13,
  author = {Fardushi Rahman and Sabina Easmin and Shahed Zaman and Hamidul Haq and Rezaul Haque Ansary and Subed Chandra Dev Sharma and Suganya Murugesu and Vikneswari Perumal},
  title = {Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh},
  journal = {Science Journal of Chemistry},
  volume = {9},
  number = {2},
  pages = {54-61},
  doi = {10.11648/j.sjc.20210902.13},
  url = {https://doi.org/10.11648/j.sjc.20210902.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20210902.13},
  abstract = {Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.},
 year = {2021}
}

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TY - JOUR
T1 - Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh
AU - Fardushi Rahman
AU - Sabina Easmin
AU - Shahed Zaman
AU - Hamidul Haq
AU - Rezaul Haque Ansary
AU - Subed Chandra Dev Sharma
AU - Suganya Murugesu
AU - Vikneswari Perumal
Y1 - 2021/04/23
PY - 2021
N1 - https://doi.org/10.11648/j.sjc.20210902.13
DO - 10.11648/j.sjc.20210902.13
T2 - Science Journal of Chemistry
JF - Science Journal of Chemistry
JO - Science Journal of Chemistry
SP - 54
EP - 61
PB - Science Publishing Group
SN - 2330-099X
UR - https://doi.org/10.11648/j.sjc.20210902.13
AB - Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.
VL - 9
IS - 2
ER -

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Author Information

Fardushi Rahman

Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh
Sabina Easmin

Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh
Shahed Zaman

Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh
Hamidul Haq

Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh
Rezaul Haque Ansary

Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh
Subed Chandra Dev Sharma

Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh
Suganya Murugesu

Institute of Tropical Agriculture and Food Security (ITAFoS), University Putra Malaysia, U. P. M. Serdang, Selangor Darul Ehsan, Malaysia
Vikneswari Perumal

Department of Pharmaceutical Technology, Faculty of Pharmacy & Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh, Perak Darul Ridzuan, Malaysia

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Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, et al. (2021). Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Science Journal of Chemistry, 9(2), 54-61. https://doi.org/10.11648/j.sjc.20210902.13

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ACS Style

Fardushi Rahman; Sabina Easmin; Shahed Zaman; Hamidul Haq; Rezaul Haque Ansary, et al. Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Sci. J. Chem. 2021, 9(2), 54-61. doi: 10.11648/j.sjc.20210902.13

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AMA Style

Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, et al. Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Sci J Chem. 2021;9(2):54-61. doi: 10.11648/j.sjc.20210902.13

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@article{10.11648/j.sjc.20210902.13,
  author = {Fardushi Rahman and Sabina Easmin and Shahed Zaman and Hamidul Haq and Rezaul Haque Ansary and Subed Chandra Dev Sharma and Suganya Murugesu and Vikneswari Perumal},
  title = {Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh},
  journal = {Science Journal of Chemistry},
  volume = {9},
  number = {2},
  pages = {54-61},
  doi = {10.11648/j.sjc.20210902.13},
  url = {https://doi.org/10.11648/j.sjc.20210902.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20210902.13},
  abstract = {Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.},
 year = {2021}
}

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