Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag

Li Dongdong; Liao Shiqi; Yuan Hongxia; Zhao Yunwang; Li Xing; Wang Jing; Wang Jin; Tian Caiping; Wei Zhengli

doi:doi:10.11648/j.sd.20160405.14

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Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag

Li Dongdong, Liao Shiqi, Yuan Hongxia, Zhao Yunwang, Li Xing, Wang Jing, Wang Jin, Tian Caiping, Wei Zhengli

Published in Science Discovery (Volume 4, Issue 5)

Received: 13 October 2016 Published: 13 October 2016

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Abstract

Severe hepatitis disease risk, mortality is high in the diagnosis of phenotypic showed the most late for hepatitis, diagnosis and treatment has been late right now, therefore, can find a effective, sensitive, rapidly and accurately identify antigen of second liver surface, the method of early diagnosis and treatment of hepatitis b virus infection has very important significance. In this article, we use mouse IgG Fc fragment nucleic acid beacon as the detection molecules to detecte HBsAg, The 5 'end of the ligand binds a double-stranded beacon sequence as detection molecules, which has specific binding capacity for mouse IgG Fc fragment. A carboxyl group of agarose beads was used as a carrier of combining with hepatitis B goat anti-human polyclonal antibody as a capture molecule to catch hepatitis B virus surface antigen in the liquid to be detected, then combining with mouse anti-human IgG immunoglobulin for HBsAg and nucleic acid beacon to form beads-Polyclonal Antibody-antigen-monoclonal antibody-beacon complexes. Hepatitis B surface antigen quantitative detection technology was achieved by using Real Time-PCR to detect nucleic acid beacon. We termed the detection technology as Immune-PCR detection technology, which is a fast, stable, accurate and sensitive technology for hepatitis B surface antigen detection，In clinic for the early diagnosis and treatment of hepatitis b virus (HBV) infection has very important significance.

Published in	Science Discovery (Volume 4, Issue 5)
DOI	10.11648/j.sd.20160405.14
Page(s)	285-291
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Carboxyl Agarose Beads, Nucleic Acid Beacon, HBsAg, Hepatitis B Mouse Anti-human IgG, Immune–PCR

References

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APA Style

Li Dongdong, Liao Shiqi, Yuan Hongxia, Zhao Yunwang, Li Xing, et al. (2016). Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag. Science Discovery, 4(5), 285-291. https://doi.org/10.11648/j.sd.20160405.14

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ACS Style

Li Dongdong; Liao Shiqi; Yuan Hongxia; Zhao Yunwang; Li Xing, et al. Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag. Sci. Discov. 2016, 4(5), 285-291. doi: 10.11648/j.sd.20160405.14

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AMA Style

Li Dongdong, Liao Shiqi, Yuan Hongxia, Zhao Yunwang, Li Xing, et al. Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag. Sci Discov. 2016;4(5):285-291. doi: 10.11648/j.sd.20160405.14

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@article{10.11648/j.sd.20160405.14,
  author = {Li Dongdong and Liao Shiqi and Yuan Hongxia and Zhao Yunwang and Li Xing and Wang Jing and Wang Jin and Tian Caiping and Wei Zhengli},
  title = {Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag},
  journal = {Science Discovery},
  volume = {4},
  number = {5},
  pages = {285-291},
  doi = {10.11648/j.sd.20160405.14},
  url = {https://doi.org/10.11648/j.sd.20160405.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160405.14},
  abstract = {Severe hepatitis disease risk, mortality is high in the diagnosis of phenotypic showed the most late for hepatitis, diagnosis and treatment has been late right now, therefore, can find a effective, sensitive, rapidly and accurately identify antigen of second liver surface, the method of early diagnosis and treatment of hepatitis b virus infection has very important significance. In this article, we use mouse IgG Fc fragment nucleic acid beacon as the detection molecules to detecte HBsAg, The 5 'end of the ligand binds a double-stranded beacon sequence as detection molecules, which has specific binding capacity for mouse IgG Fc fragment. A carboxyl group of agarose beads was used as a carrier of combining with hepatitis B goat anti-human polyclonal antibody as a capture molecule to catch hepatitis B virus surface antigen in the liquid to be detected, then combining with mouse anti-human IgG immunoglobulin for HBsAg and nucleic acid beacon to form beads-Polyclonal Antibody-antigen-monoclonal antibody-beacon complexes. Hepatitis B surface antigen quantitative detection technology was achieved by using Real Time-PCR to detect nucleic acid beacon. We termed the detection technology as Immune-PCR detection technology, which is a fast, stable, accurate and sensitive technology for hepatitis B surface antigen detection，In clinic for the early diagnosis and treatment of hepatitis b virus (HBV) infection has very important significance.},
 year = {2016}
}

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TY  - JOUR
T1  - Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag
AU  - Li Dongdong
AU  - Liao Shiqi
AU  - Yuan Hongxia
AU  - Zhao Yunwang
AU  - Li Xing
AU  - Wang Jing
AU  - Wang Jin
AU  - Tian Caiping
AU  - Wei Zhengli
Y1  - 2016/10/13
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160405.14
DO  - 10.11648/j.sd.20160405.14
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 285
EP  - 291
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160405.14
AB  - Severe hepatitis disease risk, mortality is high in the diagnosis of phenotypic showed the most late for hepatitis, diagnosis and treatment has been late right now, therefore, can find a effective, sensitive, rapidly and accurately identify antigen of second liver surface, the method of early diagnosis and treatment of hepatitis b virus infection has very important significance. In this article, we use mouse IgG Fc fragment nucleic acid beacon as the detection molecules to detecte HBsAg, The 5 'end of the ligand binds a double-stranded beacon sequence as detection molecules, which has specific binding capacity for mouse IgG Fc fragment. A carboxyl group of agarose beads was used as a carrier of combining with hepatitis B goat anti-human polyclonal antibody as a capture molecule to catch hepatitis B virus surface antigen in the liquid to be detected, then combining with mouse anti-human IgG immunoglobulin for HBsAg and nucleic acid beacon to form beads-Polyclonal Antibody-antigen-monoclonal antibody-beacon complexes. Hepatitis B surface antigen quantitative detection technology was achieved by using Real Time-PCR to detect nucleic acid beacon. We termed the detection technology as Immune-PCR detection technology, which is a fast, stable, accurate and sensitive technology for hepatitis B surface antigen detection，In clinic for the early diagnosis and treatment of hepatitis b virus (HBV) infection has very important significance.
VL  - 4
IS  - 5
ER  -

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Author Information

Li Dongdong

Gansu Province Medical Science Research Institute and College of Life Science of Cell Biology, Lanzhou University, Lanzhou, China
Liao Shiqi

Gansu Province Medical Science Research Institute, Lanzhou, China
Yuan Hongxia

Gansu Province Medical Science Research Institute, Lanzhou, China
Zhao Yunwang

Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Li Xing

Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Wang Jing

Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Wang Jin

Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Tian Caiping

Gansu Province Medical Science Research Institute, Lanzhou, China
Wei Zhengli

Gansu Province Medical Science Research Institute, Lanzhou, China

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APA Style

Li Dongdong, Liao Shiqi, Yuan Hongxia, Zhao Yunwang, Li Xing, et al. (2016). Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag. Science Discovery, 4(5), 285-291. https://doi.org/10.11648/j.sd.20160405.14

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ACS Style

Li Dongdong; Liao Shiqi; Yuan Hongxia; Zhao Yunwang; Li Xing, et al. Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag. Sci. Discov. 2016, 4(5), 285-291. doi: 10.11648/j.sd.20160405.14

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AMA Style

Li Dongdong, Liao Shiqi, Yuan Hongxia, Zhao Yunwang, Li Xing, et al. Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag. Sci Discov. 2016;4(5):285-291. doi: 10.11648/j.sd.20160405.14

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@article{10.11648/j.sd.20160405.14,
  author = {Li Dongdong and Liao Shiqi and Yuan Hongxia and Zhao Yunwang and Li Xing and Wang Jing and Wang Jin and Tian Caiping and Wei Zhengli},
  title = {Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag},
  journal = {Science Discovery},
  volume = {4},
  number = {5},
  pages = {285-291},
  doi = {10.11648/j.sd.20160405.14},
  url = {https://doi.org/10.11648/j.sd.20160405.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160405.14},
  abstract = {Severe hepatitis disease risk, mortality is high in the diagnosis of phenotypic showed the most late for hepatitis, diagnosis and treatment has been late right now, therefore, can find a effective, sensitive, rapidly and accurately identify antigen of second liver surface, the method of early diagnosis and treatment of hepatitis b virus infection has very important significance. In this article, we use mouse IgG Fc fragment nucleic acid beacon as the detection molecules to detecte HBsAg, The 5 'end of the ligand binds a double-stranded beacon sequence as detection molecules, which has specific binding capacity for mouse IgG Fc fragment. A carboxyl group of agarose beads was used as a carrier of combining with hepatitis B goat anti-human polyclonal antibody as a capture molecule to catch hepatitis B virus surface antigen in the liquid to be detected, then combining with mouse anti-human IgG immunoglobulin for HBsAg and nucleic acid beacon to form beads-Polyclonal Antibody-antigen-monoclonal antibody-beacon complexes. Hepatitis B surface antigen quantitative detection technology was achieved by using Real Time-PCR to detect nucleic acid beacon. We termed the detection technology as Immune-PCR detection technology, which is a fast, stable, accurate and sensitive technology for hepatitis B surface antigen detection，In clinic for the early diagnosis and treatment of hepatitis b virus (HBV) infection has very important significance.},
 year = {2016}
}

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TY  - JOUR
T1  - Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag
AU  - Li Dongdong
AU  - Liao Shiqi
AU  - Yuan Hongxia
AU  - Zhao Yunwang
AU  - Li Xing
AU  - Wang Jing
AU  - Wang Jin
AU  - Tian Caiping
AU  - Wei Zhengli
Y1  - 2016/10/13
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160405.14
DO  - 10.11648/j.sd.20160405.14
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 285
EP  - 291
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160405.14
AB  - Severe hepatitis disease risk, mortality is high in the diagnosis of phenotypic showed the most late for hepatitis, diagnosis and treatment has been late right now, therefore, can find a effective, sensitive, rapidly and accurately identify antigen of second liver surface, the method of early diagnosis and treatment of hepatitis b virus infection has very important significance. In this article, we use mouse IgG Fc fragment nucleic acid beacon as the detection molecules to detecte HBsAg, The 5 'end of the ligand binds a double-stranded beacon sequence as detection molecules, which has specific binding capacity for mouse IgG Fc fragment. A carboxyl group of agarose beads was used as a carrier of combining with hepatitis B goat anti-human polyclonal antibody as a capture molecule to catch hepatitis B virus surface antigen in the liquid to be detected, then combining with mouse anti-human IgG immunoglobulin for HBsAg and nucleic acid beacon to form beads-Polyclonal Antibody-antigen-monoclonal antibody-beacon complexes. Hepatitis B surface antigen quantitative detection technology was achieved by using Real Time-PCR to detect nucleic acid beacon. We termed the detection technology as Immune-PCR detection technology, which is a fast, stable, accurate and sensitive technology for hepatitis B surface antigen detection，In clinic for the early diagnosis and treatment of hepatitis b virus (HBV) infection has very important significance.
VL  - 4
IS  - 5
ER  -

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