Bismuth (Bi) is used in semiconductors and water pipes as a substitute for lead. The Bi concentration in the soil has not been investigated, and there is no environmental quality standard for Bi in Japan. We previously reported Bi accumulation and phytotoxicity in Arabidopsis thaliana (A. thaliana) and Solanum lycopersicum, which were presented that Bi disturb iron (Fe) homeostasis in both plants. However, the mechanism of Bi phytotoxicity remains unclear. We examined the toxic effect of Bi for the expression of Fe-related genes in A. thaliana. The microarray analysis revealed an increase of the Fe regulation cascade including the subgroup Ib transcription factor. We tried to clear the effect to Fe homeostasis using the Iron-Regulated Transporter 1 (IRT1) promoter knockout line. Bi inhibits the root growth and lateral root development, while Bi induces Fe contents. In wild type, Fe was located in the stem cell niche (SCN) without Bi condition. Fe location in IRT1 promoter knockout line was disturbed by 2 µM Bi treatment. Moreover, the cell death in root was found in 2 µM Bi treated-roots. These results suggest that Bi disturbed the Fe homeostasis, Fe overaccumulation enhanced cell death in A. thaliana, and Bi damaged SCN in the root tip.
| Published in | Plant (Volume 9, Issue 3) |
| DOI | 10.11648/j.plant.20210903.12 |
| Page(s) | 48-57 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Bismuth (Bi), Iron (Fe), Iron-Regulated Transporter 1 (IRT1), Stem Cell Niche (SCN), Quiescent Center (QC)
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APA Style
Makoto Nishimura, Takeshi Nagata. (2021). Phytotoxicity and Iron Homeostasis Disturbance of Bismuth on IRT1 Promoter Knockout Arabidopsis thaliana. Plant, 9(3), 48-57. https://doi.org/10.11648/j.plant.20210903.12
ACS Style
Makoto Nishimura; Takeshi Nagata. Phytotoxicity and Iron Homeostasis Disturbance of Bismuth on IRT1 Promoter Knockout Arabidopsis thaliana. Plant. 2021, 9(3), 48-57. doi: 10.11648/j.plant.20210903.12
AMA Style
Makoto Nishimura, Takeshi Nagata. Phytotoxicity and Iron Homeostasis Disturbance of Bismuth on IRT1 Promoter Knockout Arabidopsis thaliana. Plant. 2021;9(3):48-57. doi: 10.11648/j.plant.20210903.12
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Download@article{10.11648/j.plant.20210903.12,
author = {Makoto Nishimura and Takeshi Nagata},
title = {Phytotoxicity and Iron Homeostasis Disturbance of Bismuth on IRT1 Promoter Knockout Arabidopsis thaliana},
journal = {Plant},
volume = {9},
number = {3},
pages = {48-57},
doi = {10.11648/j.plant.20210903.12},
url = {https://doi.org/10.11648/j.plant.20210903.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20210903.12},
abstract = {Bismuth (Bi) is used in semiconductors and water pipes as a substitute for lead. The Bi concentration in the soil has not been investigated, and there is no environmental quality standard for Bi in Japan. We previously reported Bi accumulation and phytotoxicity in Arabidopsis thaliana (A. thaliana) and Solanum lycopersicum, which were presented that Bi disturb iron (Fe) homeostasis in both plants. However, the mechanism of Bi phytotoxicity remains unclear. We examined the toxic effect of Bi for the expression of Fe-related genes in A. thaliana. The microarray analysis revealed an increase of the Fe regulation cascade including the subgroup Ib transcription factor. We tried to clear the effect to Fe homeostasis using the Iron-Regulated Transporter 1 (IRT1) promoter knockout line. Bi inhibits the root growth and lateral root development, while Bi induces Fe contents. In wild type, Fe was located in the stem cell niche (SCN) without Bi condition. Fe location in IRT1 promoter knockout line was disturbed by 2 µM Bi treatment. Moreover, the cell death in root was found in 2 µM Bi treated-roots. These results suggest that Bi disturbed the Fe homeostasis, Fe overaccumulation enhanced cell death in A. thaliana, and Bi damaged SCN in the root tip.},
year = {2021}
}
TY - JOUR T1 - Phytotoxicity and Iron Homeostasis Disturbance of Bismuth on IRT1 Promoter Knockout Arabidopsis thaliana AU - Makoto Nishimura AU - Takeshi Nagata Y1 - 2021/08/04 PY - 2021 N1 - https://doi.org/10.11648/j.plant.20210903.12 DO - 10.11648/j.plant.20210903.12 T2 - Plant JF - Plant JO - Plant SP - 48 EP - 57 PB - Science Publishing Group SN - 2331-0677 UR - https://doi.org/10.11648/j.plant.20210903.12 AB - Bismuth (Bi) is used in semiconductors and water pipes as a substitute for lead. The Bi concentration in the soil has not been investigated, and there is no environmental quality standard for Bi in Japan. We previously reported Bi accumulation and phytotoxicity in Arabidopsis thaliana (A. thaliana) and Solanum lycopersicum, which were presented that Bi disturb iron (Fe) homeostasis in both plants. However, the mechanism of Bi phytotoxicity remains unclear. We examined the toxic effect of Bi for the expression of Fe-related genes in A. thaliana. The microarray analysis revealed an increase of the Fe regulation cascade including the subgroup Ib transcription factor. We tried to clear the effect to Fe homeostasis using the Iron-Regulated Transporter 1 (IRT1) promoter knockout line. Bi inhibits the root growth and lateral root development, while Bi induces Fe contents. In wild type, Fe was located in the stem cell niche (SCN) without Bi condition. Fe location in IRT1 promoter knockout line was disturbed by 2 µM Bi treatment. Moreover, the cell death in root was found in 2 µM Bi treated-roots. These results suggest that Bi disturbed the Fe homeostasis, Fe overaccumulation enhanced cell death in A. thaliana, and Bi damaged SCN in the root tip. VL - 9 IS - 3 ER -
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