Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes

Esra Maltas; Aslı Dageri; Hasibe Cingilli Vural; Salih Yildiz

doi:doi:10.11648/j.plant.20150301.11

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Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes

Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz

Published in Plant (Volume 3, Issue 1)

Received: 21 August 2014 Accepted: 9 September 2014 Published: 15 December 2014

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Abstract

Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.

Published in	Plant (Volume 3, Issue 1)
DOI	10.11648/j.plant.20150301.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Echinacea Purpurea and Echinacea Pallida, Molecular Marker, PCR, nDNA Antioxidant Activity, Fatty acid, Flavonoid

References

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APA Style

Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz. (2014). Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant, 3(1), 1-7. https://doi.org/10.11648/j.plant.20150301.11

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ACS Style

Esra Maltas; Aslı Dageri; Hasibe Cingilli Vural; Salih Yildiz. Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant. 2014, 3(1), 1-7. doi: 10.11648/j.plant.20150301.11

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AMA Style

Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz. Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant. 2014;3(1):1-7. doi: 10.11648/j.plant.20150301.11

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@article{10.11648/j.plant.20150301.11,
  author = {Esra Maltas and Aslı Dageri and Hasibe Cingilli Vural and Salih Yildiz},
  title = {Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes},
  journal = {Plant},
  volume = {3},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.plant.20150301.11},
  url = {https://doi.org/10.11648/j.plant.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20150301.11},
  abstract = {Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.},
 year = {2014}
}

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TY  - JOUR
T1  - Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes
AU  - Esra Maltas
AU  - Aslı Dageri
AU  - Hasibe Cingilli Vural
AU  - Salih Yildiz
Y1  - 2014/12/15
PY  - 2014
N1  - https://doi.org/10.11648/j.plant.20150301.11
DO  - 10.11648/j.plant.20150301.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20150301.11
AB  - Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.
VL  - 3
IS  - 1
ER  -

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Author Information

Esra Maltas

Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey
Aslı Dageri
Hasibe Cingilli Vural

Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey
Salih Yildiz

Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey

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Plain Text BibTeX RIS

APA Style

Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz. (2014). Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant, 3(1), 1-7. https://doi.org/10.11648/j.plant.20150301.11

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ACS Style

Esra Maltas; Aslı Dageri; Hasibe Cingilli Vural; Salih Yildiz. Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant. 2014, 3(1), 1-7. doi: 10.11648/j.plant.20150301.11

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AMA Style

Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz. Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant. 2014;3(1):1-7. doi: 10.11648/j.plant.20150301.11

Copy | Download

@article{10.11648/j.plant.20150301.11,
  author = {Esra Maltas and Aslı Dageri and Hasibe Cingilli Vural and Salih Yildiz},
  title = {Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes},
  journal = {Plant},
  volume = {3},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.plant.20150301.11},
  url = {https://doi.org/10.11648/j.plant.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20150301.11},
  abstract = {Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes
AU  - Esra Maltas
AU  - Aslı Dageri
AU  - Hasibe Cingilli Vural
AU  - Salih Yildiz
Y1  - 2014/12/15
PY  - 2014
N1  - https://doi.org/10.11648/j.plant.20150301.11
DO  - 10.11648/j.plant.20150301.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20150301.11
AB  - Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.
VL  - 3
IS  - 1
ER  -

Copy | Download