Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections

Klemens Vertesich; Thomas Mayrhofer; Reinhard Windhager; Klaus-Dieter Kühn

doi:doi:10.11648/j.js.20180606.11

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Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections

Klemens Vertesich, Thomas Mayrhofer, Reinhard Windhager, Klaus-Dieter Kühn

Published in Journal of Surgery (Volume 6, Issue 6)

Received: 9 August 2018 Accepted: 23 August 2018 Published: 7 November 2018

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Abstract

Prosthetic joint infection represents a major issue in arthroplasty. Local anti-infective treatment is not established in cementless prosthetic surgery. The aim of this study was to perform simulate a perioperative application of agent-fatty acid complexes on surfaces of primary and revision prosthetic material. Further, it was aimed to investigate the efficacy of these coatings by in vitro microbiological tests. Coating of cemetless titanium prostheses with gentamicin-palmitate and octenidine-laurate was performed by using a spray gun system. Coating with vancomycin eluted in trilaurin was performed by dipping of the prostheses in the solution. The prostheses were incubated in phosphate buffered saline for 7 days. Microbiological testing was performed with inhibition areolae testing for S. aureus, S. epidermidis, MRSA and C. albicans. Coating of prosthetic material was reliable and reproducible with two different techniques, dipping and spraying. The surface-concentrations of agents have reached 195μg/cm2 for gentamicin, 460μg/cm² for octenidine and 323μg/cm² for vancomycin. Agents inhibited S. epidermidis and S. aureus growth for seven days, C. albicans for three days and MRSA for two days. Agent-fatty acid coatings used in this study represent a biodegradable layer with good biocompatibility and comparable anti-infective efficacy as in cemented surgery due to the use of established agents, even if low concentrations are used. Modular and individual anti-infective coating was reproducibly and reliably performed by dipping coating, which may represent a potential perioperative coating approach.

Published in	Journal of Surgery (Volume 6, Issue 6)
DOI	10.11648/j.js.20180606.11
Page(s)	146-153
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Anti-Infective Coating, Biodegradable Coating, Prosthetic Joint Infection, Gentamicin, Vancomycin, Octenidine

References

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APA Style

Klemens Vertesich, Thomas Mayrhofer, Reinhard Windhager, Klaus-Dieter Kühn. (2018). Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections. Journal of Surgery, 6(6), 146-153. https://doi.org/10.11648/j.js.20180606.11

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ACS Style

Klemens Vertesich; Thomas Mayrhofer; Reinhard Windhager; Klaus-Dieter Kühn. Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections. J. Surg. 2018, 6(6), 146-153. doi: 10.11648/j.js.20180606.11

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AMA Style

Klemens Vertesich, Thomas Mayrhofer, Reinhard Windhager, Klaus-Dieter Kühn. Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections. J Surg. 2018;6(6):146-153. doi: 10.11648/j.js.20180606.11

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@article{10.11648/j.js.20180606.11,
  author = {Klemens Vertesich and Thomas Mayrhofer and Reinhard Windhager and Klaus-Dieter Kühn},
  title = {Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections},
  journal = {Journal of Surgery},
  volume = {6},
  number = {6},
  pages = {146-153},
  doi = {10.11648/j.js.20180606.11},
  url = {https://doi.org/10.11648/j.js.20180606.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.js.20180606.11},
  abstract = {Prosthetic joint infection represents a major issue in arthroplasty. Local anti-infective treatment is not established in cementless prosthetic surgery. The aim of this study was to perform simulate a perioperative application of agent-fatty acid complexes on surfaces of primary and revision prosthetic material. Further, it was aimed to investigate the efficacy of these coatings by in vitro microbiological tests. Coating of cemetless titanium prostheses with gentamicin-palmitate and octenidine-laurate was performed by using a spray gun system. Coating with vancomycin eluted in trilaurin was performed by dipping of the prostheses in the solution. The prostheses were incubated in phosphate buffered saline for 7 days. Microbiological testing was performed with inhibition areolae testing for S. aureus, S. epidermidis, MRSA and C. albicans. Coating of prosthetic material was reliable and reproducible with two different techniques, dipping and spraying. The surface-concentrations of agents have reached 195μg/cm2 for gentamicin, 460μg/cm2 for octenidine and 323μg/cm2 for vancomycin. Agents inhibited S. epidermidis and S. aureus growth for seven days, C. albicans for three days and MRSA for two days. Agent-fatty acid coatings used in this study represent a biodegradable layer with good biocompatibility and comparable anti-infective efficacy as in cemented surgery due to the use of established agents, even if low concentrations are used. Modular and individual anti-infective coating was reproducibly and reliably performed by dipping coating, which may represent a potential perioperative coating approach.},
 year = {2018}
}

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TY - JOUR
T1 - Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections
AU - Klemens Vertesich
AU - Thomas Mayrhofer
AU - Reinhard Windhager
AU - Klaus-Dieter Kühn
Y1 - 2018/11/07
PY - 2018
N1 - https://doi.org/10.11648/j.js.20180606.11
DO - 10.11648/j.js.20180606.11
T2 - Journal of Surgery
JF - Journal of Surgery
JO - Journal of Surgery
SP - 146
EP - 153
PB - Science Publishing Group
SN - 2330-0930
UR - https://doi.org/10.11648/j.js.20180606.11
AB - Prosthetic joint infection represents a major issue in arthroplasty. Local anti-infective treatment is not established in cementless prosthetic surgery. The aim of this study was to perform simulate a perioperative application of agent-fatty acid complexes on surfaces of primary and revision prosthetic material. Further, it was aimed to investigate the efficacy of these coatings by in vitro microbiological tests. Coating of cemetless titanium prostheses with gentamicin-palmitate and octenidine-laurate was performed by using a spray gun system. Coating with vancomycin eluted in trilaurin was performed by dipping of the prostheses in the solution. The prostheses were incubated in phosphate buffered saline for 7 days. Microbiological testing was performed with inhibition areolae testing for S. aureus, S. epidermidis, MRSA and C. albicans. Coating of prosthetic material was reliable and reproducible with two different techniques, dipping and spraying. The surface-concentrations of agents have reached 195μg/cm2 for gentamicin, 460μg/cm2 for octenidine and 323μg/cm2 for vancomycin. Agents inhibited S. epidermidis and S. aureus growth for seven days, C. albicans for three days and MRSA for two days. Agent-fatty acid coatings used in this study represent a biodegradable layer with good biocompatibility and comparable anti-infective efficacy as in cemented surgery due to the use of established agents, even if low concentrations are used. Modular and individual anti-infective coating was reproducibly and reliably performed by dipping coating, which may represent a potential perioperative coating approach.
VL - 6
IS - 6
ER -

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Author Information

Klemens Vertesich

Department of Orthopaedics and Trauma Surgery, Medical University of Vienna, Vienna, Austria
Thomas Mayrhofer

Department of Orthopaedics and Trauma Surgery, Medical University of Vienna, Vienna, Austria
Reinhard Windhager

Department of Orthopaedics and Trauma Surgery, Medical University of Vienna, Vienna, Austria
Klaus-Dieter Kühn

Department of Orthopaedics and Trauma Surgery, Medical University of Graz, Graz, Austria

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APA Style

Klemens Vertesich, Thomas Mayrhofer, Reinhard Windhager, Klaus-Dieter Kühn. (2018). Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections. Journal of Surgery, 6(6), 146-153. https://doi.org/10.11648/j.js.20180606.11

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ACS Style

Klemens Vertesich; Thomas Mayrhofer; Reinhard Windhager; Klaus-Dieter Kühn. Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections. J. Surg. 2018, 6(6), 146-153. doi: 10.11648/j.js.20180606.11

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AMA Style

Klemens Vertesich, Thomas Mayrhofer, Reinhard Windhager, Klaus-Dieter Kühn. Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections. J Surg. 2018;6(6):146-153. doi: 10.11648/j.js.20180606.11

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@article{10.11648/j.js.20180606.11,
  author = {Klemens Vertesich and Thomas Mayrhofer and Reinhard Windhager and Klaus-Dieter Kühn},
  title = {Accessible Agent-Fatty Acid Coatings of Titanium Prostheses for Local Prevention and Treatment of Anti-Microbial Infections},
  journal = {Journal of Surgery},
  volume = {6},
  number = {6},
  pages = {146-153},
  doi = {10.11648/j.js.20180606.11},
  url = {https://doi.org/10.11648/j.js.20180606.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.js.20180606.11},
  abstract = {Prosthetic joint infection represents a major issue in arthroplasty. Local anti-infective treatment is not established in cementless prosthetic surgery. The aim of this study was to perform simulate a perioperative application of agent-fatty acid complexes on surfaces of primary and revision prosthetic material. Further, it was aimed to investigate the efficacy of these coatings by in vitro microbiological tests. Coating of cemetless titanium prostheses with gentamicin-palmitate and octenidine-laurate was performed by using a spray gun system. Coating with vancomycin eluted in trilaurin was performed by dipping of the prostheses in the solution. The prostheses were incubated in phosphate buffered saline for 7 days. Microbiological testing was performed with inhibition areolae testing for S. aureus, S. epidermidis, MRSA and C. albicans. Coating of prosthetic material was reliable and reproducible with two different techniques, dipping and spraying. The surface-concentrations of agents have reached 195μg/cm2 for gentamicin, 460μg/cm2 for octenidine and 323μg/cm2 for vancomycin. Agents inhibited S. epidermidis and S. aureus growth for seven days, C. albicans for three days and MRSA for two days. Agent-fatty acid coatings used in this study represent a biodegradable layer with good biocompatibility and comparable anti-infective efficacy as in cemented surgery due to the use of established agents, even if low concentrations are used. Modular and individual anti-infective coating was reproducibly and reliably performed by dipping coating, which may represent a potential perioperative coating approach.},
 year = {2018}
}

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