The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (<i>Ipomoea batatas</i> Lam) from Benin

Arsène Mahoutondji Doussoh; Justine Sossou Dangou; Arnaud Agbidinoukoun; Serge Sètondji Houedjissin; Corneille Ahanhanzo

doi:doi:10.11648/j.jps.20180603.13

The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin

Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo

Published in Journal of Plant Sciences (Volume 6, Issue 3)

Received: 25 June 2018 Accepted: 11 July 2018 Published: 6 August 2018

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Abstract

Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.

Published in	Journal of Plant Sciences (Volume 6, Issue 3)
DOI	10.11648/j.jps.20180603.13
Page(s)	93-100
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Sweet Potato, Ex situ Preservation, Artificial Seeds, Shoot Apices, Benin

References

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Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. (2018). The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. Journal of Plant Sciences, 6(3), 93-100. https://doi.org/10.11648/j.jps.20180603.13

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ACS Style

Arsène Mahoutondji Doussoh; Justine Sossou Dangou; Arnaud Agbidinoukoun; Serge Sètondji Houedjissin; Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J. Plant Sci. 2018, 6(3), 93-100. doi: 10.11648/j.jps.20180603.13

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AMA Style

Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J Plant Sci. 2018;6(3):93-100. doi: 10.11648/j.jps.20180603.13

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@article{10.11648/j.jps.20180603.13,
  author = {Arsène Mahoutondji Doussoh and Justine Sossou Dangou and Arnaud Agbidinoukoun and Serge Sètondji Houedjissin and Corneille Ahanhanzo},
  title = {The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin},
  journal = {Journal of Plant Sciences},
  volume = {6},
  number = {3},
  pages = {93-100},
  doi = {10.11648/j.jps.20180603.13},
  url = {https://doi.org/10.11648/j.jps.20180603.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20180603.13},
  abstract = {Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.},
 year = {2018}
}

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TY - JOUR
T1 - The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin
AU - Arsène Mahoutondji Doussoh
AU - Justine Sossou Dangou
AU - Arnaud Agbidinoukoun
AU - Serge Sètondji Houedjissin
AU - Corneille Ahanhanzo
Y1 - 2018/08/06
PY - 2018
N1 - https://doi.org/10.11648/j.jps.20180603.13
DO - 10.11648/j.jps.20180603.13
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 93
EP - 100
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20180603.13
AB - Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.
VL - 6
IS - 3
ER -

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Author Information

Arsène Mahoutondji Doussoh

Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin
Justine Sossou Dangou

Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Laboratory of Research in Applied Biology, Abomey-Calavi, Benin
Arnaud Agbidinoukoun

Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin
Serge Sètondji Houedjissin

Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin
Corneille Ahanhanzo

Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

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APA Style

Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. (2018). The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. Journal of Plant Sciences, 6(3), 93-100. https://doi.org/10.11648/j.jps.20180603.13

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ACS Style

Arsène Mahoutondji Doussoh; Justine Sossou Dangou; Arnaud Agbidinoukoun; Serge Sètondji Houedjissin; Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J. Plant Sci. 2018, 6(3), 93-100. doi: 10.11648/j.jps.20180603.13

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AMA Style

Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J Plant Sci. 2018;6(3):93-100. doi: 10.11648/j.jps.20180603.13

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@article{10.11648/j.jps.20180603.13,
  author = {Arsène Mahoutondji Doussoh and Justine Sossou Dangou and Arnaud Agbidinoukoun and Serge Sètondji Houedjissin and Corneille Ahanhanzo},
  title = {The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin},
  journal = {Journal of Plant Sciences},
  volume = {6},
  number = {3},
  pages = {93-100},
  doi = {10.11648/j.jps.20180603.13},
  url = {https://doi.org/10.11648/j.jps.20180603.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20180603.13},
  abstract = {Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.},
 year = {2018}
}

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