Vernonia amygdalina is a vegetable and medicinal plant used to treat various ailments such as diabetes, malaria, gastrointestinal disorders, and parasitic infections. The present study investigated the effect of supplementing different concentrations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) either alone or in combination to Murashige and Skoog (MS) medium on in vitro plantlet regeneration of V. amygdalina from nodal explants. Control treatment without plant growth regulators was ideal for in vitro plantlet regeneration of V. amygdalina. In vitro plantlets regenerated from nodal explants supplemented with BAP and/or NAA showed growth abnormalities including chlorosis, basal callus, and excessive adventitious rooting. Callus cultures were induced from leaf explants on MS medium supplemented with different concentrations of BAP, NAA, and 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or in combination. Maximum callus induction frequency (100%) was recorded in leaf explants cultured on MS medium supplemented with 0.5 – 2.0 mg/L 2,4-D. Fresh weight of calli increased up to 11-fold when treated with 0.5 mg/L 2,4-D after 8 weeks of culture.
| Published in | Journal of Plant Sciences (Volume 6, Issue 1) |
| DOI | 10.11648/j.jps.20180601.11 |
| Page(s) | 1-6 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Vernonia amygdalina, Plantlet Regeneration, Callus
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APA Style
Chen Mei-Yin, Hamsawi Sani. (2017). In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile. Journal of Plant Sciences, 6(1), 1-6. https://doi.org/10.11648/j.jps.20180601.11
ACS Style
Chen Mei-Yin; Hamsawi Sani. In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile. J. Plant Sci. 2017, 6(1), 1-6. doi: 10.11648/j.jps.20180601.11
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Download@article{10.11648/j.jps.20180601.11,
author = {Chen Mei-Yin and Hamsawi Sani},
title = {In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile},
journal = {Journal of Plant Sciences},
volume = {6},
number = {1},
pages = {1-6},
doi = {10.11648/j.jps.20180601.11},
url = {https://doi.org/10.11648/j.jps.20180601.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20180601.11},
abstract = {Vernonia amygdalina is a vegetable and medicinal plant used to treat various ailments such as diabetes, malaria, gastrointestinal disorders, and parasitic infections. The present study investigated the effect of supplementing different concentrations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) either alone or in combination to Murashige and Skoog (MS) medium on in vitro plantlet regeneration of V. amygdalina from nodal explants. Control treatment without plant growth regulators was ideal for in vitro plantlet regeneration of V. amygdalina. In vitro plantlets regenerated from nodal explants supplemented with BAP and/or NAA showed growth abnormalities including chlorosis, basal callus, and excessive adventitious rooting. Callus cultures were induced from leaf explants on MS medium supplemented with different concentrations of BAP, NAA, and 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or in combination. Maximum callus induction frequency (100%) was recorded in leaf explants cultured on MS medium supplemented with 0.5 – 2.0 mg/L 2,4-D. Fresh weight of calli increased up to 11-fold when treated with 0.5 mg/L 2,4-D after 8 weeks of culture.},
year = {2017}
}
TY - JOUR T1 - In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile AU - Chen Mei-Yin AU - Hamsawi Sani Y1 - 2017/12/20 PY - 2017 N1 - https://doi.org/10.11648/j.jps.20180601.11 DO - 10.11648/j.jps.20180601.11 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 1 EP - 6 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20180601.11 AB - Vernonia amygdalina is a vegetable and medicinal plant used to treat various ailments such as diabetes, malaria, gastrointestinal disorders, and parasitic infections. The present study investigated the effect of supplementing different concentrations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) either alone or in combination to Murashige and Skoog (MS) medium on in vitro plantlet regeneration of V. amygdalina from nodal explants. Control treatment without plant growth regulators was ideal for in vitro plantlet regeneration of V. amygdalina. In vitro plantlets regenerated from nodal explants supplemented with BAP and/or NAA showed growth abnormalities including chlorosis, basal callus, and excessive adventitious rooting. Callus cultures were induced from leaf explants on MS medium supplemented with different concentrations of BAP, NAA, and 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or in combination. Maximum callus induction frequency (100%) was recorded in leaf explants cultured on MS medium supplemented with 0.5 – 2.0 mg/L 2,4-D. Fresh weight of calli increased up to 11-fold when treated with 0.5 mg/L 2,4-D after 8 weeks of culture. VL - 6 IS - 1 ER -
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