Moringa oleifera, the Miracle Tree is rich in all nutrients and minerals. It has prominent distribution of secondary metabolites like polysaccharides, phenols and mucilage, which makes extraction of RNA quite difficult. A high quality and pure RNA is prerequisite for the study of high through put transcriptomics and functional genomics. A protocol for isolation of highly qualitative and quantitative RNA from M. oleifera was optimized by comparing five different methods like Trizol, Guanidine hydrochloride, combined Trizol and Guanidine hydrochloride, modified CTAB and hot phenol method. The combined Guanidine hydrochloride and Trizol method gave good yield and pure RNA based on the absorbance A260 value 2.0. The model plant Nicotiana tabacum served as a positive control in which the Trizol method yielded a good quality and quantity RNA. The present study is a preliminary step for studying the function and expression pattern of the genes. This is the first report on the comparison of different RNA extraction methods in M. oleifera to our knowledge.
| Published in | Journal of Plant Sciences (Volume 5, Issue 2) |
| DOI | 10.11648/j.jps.20170502.14 |
| Page(s) | 68-74 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Moringa oleifera, Nicotiana tabacum, RNA Isolation, Trizol, Guanidine Hydrochloride, RT-PCR
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APA Style
Preethi Praba Umesh, Mohd Akram Ansari, Ganapathi Sridevi. (2017). An Efficient Method for High Quality RNA Extraction from Moringa oleifera. Journal of Plant Sciences, 5(2), 68-74. https://doi.org/10.11648/j.jps.20170502.14
ACS Style
Preethi Praba Umesh; Mohd Akram Ansari; Ganapathi Sridevi. An Efficient Method for High Quality RNA Extraction from Moringa oleifera. J. Plant Sci. 2017, 5(2), 68-74. doi: 10.11648/j.jps.20170502.14
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Download@article{10.11648/j.jps.20170502.14,
author = {Preethi Praba Umesh and Mohd Akram Ansari and Ganapathi Sridevi},
title = {An Efficient Method for High Quality RNA Extraction from Moringa oleifera},
journal = {Journal of Plant Sciences},
volume = {5},
number = {2},
pages = {68-74},
doi = {10.11648/j.jps.20170502.14},
url = {https://doi.org/10.11648/j.jps.20170502.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20170502.14},
abstract = {Moringa oleifera, the Miracle Tree is rich in all nutrients and minerals. It has prominent distribution of secondary metabolites like polysaccharides, phenols and mucilage, which makes extraction of RNA quite difficult. A high quality and pure RNA is prerequisite for the study of high through put transcriptomics and functional genomics. A protocol for isolation of highly qualitative and quantitative RNA from M. oleifera was optimized by comparing five different methods like Trizol, Guanidine hydrochloride, combined Trizol and Guanidine hydrochloride, modified CTAB and hot phenol method. The combined Guanidine hydrochloride and Trizol method gave good yield and pure RNA based on the absorbance A260 value 2.0. The model plant Nicotiana tabacum served as a positive control in which the Trizol method yielded a good quality and quantity RNA. The present study is a preliminary step for studying the function and expression pattern of the genes. This is the first report on the comparison of different RNA extraction methods in M. oleifera to our knowledge.},
year = {2017}
}
TY - JOUR T1 - An Efficient Method for High Quality RNA Extraction from Moringa oleifera AU - Preethi Praba Umesh AU - Mohd Akram Ansari AU - Ganapathi Sridevi Y1 - 2017/03/28 PY - 2017 N1 - https://doi.org/10.11648/j.jps.20170502.14 DO - 10.11648/j.jps.20170502.14 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 68 EP - 74 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20170502.14 AB - Moringa oleifera, the Miracle Tree is rich in all nutrients and minerals. It has prominent distribution of secondary metabolites like polysaccharides, phenols and mucilage, which makes extraction of RNA quite difficult. A high quality and pure RNA is prerequisite for the study of high through put transcriptomics and functional genomics. A protocol for isolation of highly qualitative and quantitative RNA from M. oleifera was optimized by comparing five different methods like Trizol, Guanidine hydrochloride, combined Trizol and Guanidine hydrochloride, modified CTAB and hot phenol method. The combined Guanidine hydrochloride and Trizol method gave good yield and pure RNA based on the absorbance A260 value 2.0. The model plant Nicotiana tabacum served as a positive control in which the Trizol method yielded a good quality and quantity RNA. The present study is a preliminary step for studying the function and expression pattern of the genes. This is the first report on the comparison of different RNA extraction methods in M. oleifera to our knowledge. VL - 5 IS - 2 ER -
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