Genetic Diversity in Pigeon Pea (<i>Cajanuscajan</i> (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins

Agbolade James Oludare; Adekoya Modinat Adejoke; David Oyinade Aderoju; Chukwuma Deborah Moradeke; Komolafe Ronke Justina; Olaiya Aderonke Eunice

doi:doi:10.11648/j.jps.20170502.11

Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins

Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, Olaiya Aderonke Eunice

Published in Journal of Plant Sciences (Volume 5, Issue 2)

Received: 8 September 2016 Accepted: 7 February 2017 Published: 14 March 2017

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Abstract

Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH₂PO₄) and 0.067 of base (Na₂HPO₄) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P<0.05) while samples PP1, PP4 and PP7 showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).

Published in	Journal of Plant Sciences (Volume 5, Issue 2)
DOI	10.11648/j.jps.20170502.11
Page(s)	48-55
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Accessions, Gel Electrophoresis, Intra-Specific, Germplasm, Genetic Diversity

References

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APA Style

Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, et al. (2017). Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. Journal of Plant Sciences, 5(2), 48-55. https://doi.org/10.11648/j.jps.20170502.11

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ACS Style

Agbolade James Oludare; Adekoya Modinat Adejoke; David Oyinade Aderoju; Chukwuma Deborah Moradeke; Komolafe Ronke Justina, et al. Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. J. Plant Sci. 2017, 5(2), 48-55. doi: 10.11648/j.jps.20170502.11

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AMA Style

Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, et al. Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. J Plant Sci. 2017;5(2):48-55. doi: 10.11648/j.jps.20170502.11

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@article{10.11648/j.jps.20170502.11,
  author = {Agbolade James Oludare and Adekoya Modinat Adejoke and David Oyinade Aderoju and Chukwuma Deborah Moradeke and Komolafe Ronke Justina and Olaiya Aderonke Eunice},
  title = {Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins},
  journal = {Journal of Plant Sciences},
  volume = {5},
  number = {2},
  pages = {48-55},
  doi = {10.11648/j.jps.20170502.11},
  url = {https://doi.org/10.11648/j.jps.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20170502.11},
  abstract = {Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).},
 year = {2017}
}

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TY  - JOUR
T1  - Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins
AU  - Agbolade James Oludare
AU  - Adekoya Modinat Adejoke
AU  - David Oyinade Aderoju
AU  - Chukwuma Deborah Moradeke
AU  - Komolafe Ronke Justina
AU  - Olaiya Aderonke Eunice
Y1  - 2017/03/14
PY  - 2017
N1  - https://doi.org/10.11648/j.jps.20170502.11
DO  - 10.11648/j.jps.20170502.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 48
EP  - 55
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20170502.11
AB  - Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).
VL  - 5
IS  - 2
ER  -

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Author Information

Agbolade James Oludare

Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria
Adekoya Modinat Adejoke

Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria
David Oyinade Aderoju

Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria
Chukwuma Deborah Moradeke

Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria
Komolafe Ronke Justina

Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria
Olaiya Aderonke Eunice

Department of Plant Science and Biotechnology, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria

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APA Style

Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, et al. (2017). Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. Journal of Plant Sciences, 5(2), 48-55. https://doi.org/10.11648/j.jps.20170502.11

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ACS Style

Agbolade James Oludare; Adekoya Modinat Adejoke; David Oyinade Aderoju; Chukwuma Deborah Moradeke; Komolafe Ronke Justina, et al. Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. J. Plant Sci. 2017, 5(2), 48-55. doi: 10.11648/j.jps.20170502.11

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AMA Style

Agbolade James Oludare, Adekoya Modinat Adejoke, David Oyinade Aderoju, Chukwuma Deborah Moradeke, Komolafe Ronke Justina, et al. Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins. J Plant Sci. 2017;5(2):48-55. doi: 10.11648/j.jps.20170502.11

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@article{10.11648/j.jps.20170502.11,
  author = {Agbolade James Oludare and Adekoya Modinat Adejoke and David Oyinade Aderoju and Chukwuma Deborah Moradeke and Komolafe Ronke Justina and Olaiya Aderonke Eunice},
  title = {Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins},
  journal = {Journal of Plant Sciences},
  volume = {5},
  number = {2},
  pages = {48-55},
  doi = {10.11648/j.jps.20170502.11},
  url = {https://doi.org/10.11648/j.jps.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20170502.11},
  abstract = {Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).},
 year = {2017}
}

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TY  - JOUR
T1  - Genetic Diversity in Pigeon Pea (Cajanuscajan (L.) Millspaugh Germplasm Revealed by Gel Electrophoresis of the Seed Proteins
AU  - Agbolade James Oludare
AU  - Adekoya Modinat Adejoke
AU  - David Oyinade Aderoju
AU  - Chukwuma Deborah Moradeke
AU  - Komolafe Ronke Justina
AU  - Olaiya Aderonke Eunice
Y1  - 2017/03/14
PY  - 2017
N1  - https://doi.org/10.11648/j.jps.20170502.11
DO  - 10.11648/j.jps.20170502.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 48
EP  - 55
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20170502.11
AB  - Ten accessions of Pigeon pea (Cajanuscajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the ten accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions PP2, PP3, PP5, PP6, PP8 and PP9 have molecular weights not significantly different from one another (P0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanuscajan).
VL  - 5
IS  - 2
ER  -

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