Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars.
| Published in | Journal of Plant Sciences (Volume 4, Issue 6) |
| DOI | 10.11648/j.jps.20160406.13 |
| Page(s) | 146-152 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Bacterial Leaf Blight (BLB), Restriction Fragment Length Polymorphism (RFLP), Resistance
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APA Style
M. H. M. Mubassir, Khondoker M. Nasiruddin, Nazmul Hoque Shahin, Shamsun Nahar Begum, Manas Kanti Saha, et al. (2016). Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. Journal of Plant Sciences, 4(6), 146-152. https://doi.org/10.11648/j.jps.20160406.13
ACS Style
M. H. M. Mubassir; Khondoker M. Nasiruddin; Nazmul Hoque Shahin; Shamsun Nahar Begum; Manas Kanti Saha, et al. Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. J. Plant Sci. 2016, 4(6), 146-152. doi: 10.11648/j.jps.20160406.13
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Download@article{10.11648/j.jps.20160406.13,
author = {M. H. M. Mubassir and Khondoker M. Nasiruddin and Nazmul Hoque Shahin and Shamsun Nahar Begum and Manas Kanti Saha and A. Q. M. Bazlur Rashid},
title = {Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties},
journal = {Journal of Plant Sciences},
volume = {4},
number = {6},
pages = {146-152},
doi = {10.11648/j.jps.20160406.13},
url = {https://doi.org/10.11648/j.jps.20160406.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160406.13},
abstract = {Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars.},
year = {2016}
}
TY - JOUR T1 - Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties AU - M. H. M. Mubassir AU - Khondoker M. Nasiruddin AU - Nazmul Hoque Shahin AU - Shamsun Nahar Begum AU - Manas Kanti Saha AU - A. Q. M. Bazlur Rashid Y1 - 2016/11/16 PY - 2016 N1 - https://doi.org/10.11648/j.jps.20160406.13 DO - 10.11648/j.jps.20160406.13 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 146 EP - 152 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20160406.13 AB - Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars. VL - 4 IS - 6 ER -
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