The Protein Profile of the Plantlets of <i>Spathoglottis plicata</i> Bl. Induced Resistance to <i>Fusarium oxysporum</i>

Endang Nurcahyani; R. Agustrina; T. T. Handayani

doi:doi:10.11648/j.jps.20160405.12

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The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum

Endang Nurcahyani, R. Agustrina, T. T. Handayani

Published in Journal of Plant Sciences (Volume 4, Issue 5)

Received: 18 July 2016 Accepted: 4 August 2016 Published: 21 August 2016

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Abstract

Ground orchid (Spathoglottis plicata) cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum (Fo). A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin and Went (VW) medium containing fusaric acid (FA) at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm), and there were indications of FA tolerant of the selected plantlets. This study aims to describe the formation mechanism of Induced Resistance by analysing the protein profile of the plantlet. The protein profile was analysed by SDS-PAGE methods. The results showed that protein profiles of explants leaves was significantly different from the control. There was an initiation of a new protein bands (± 19 kD) indicating the formation of PR-protein (peroxidase) on the plantlets which responsible for S. plicata resistant to Fo.

Published in	Journal of Plant Sciences (Volume 4, Issue 5)
DOI	10.11648/j.jps.20160405.12
Page(s)	102-105
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Protein Profile, Spathoglottis plicata Bl., In Vitro, Induced Resistance, Fusarium oxysporum

References

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APA Style

Endang Nurcahyani, R. Agustrina, T. T. Handayani. (2016). The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. Journal of Plant Sciences, 4(5), 102-105. https://doi.org/10.11648/j.jps.20160405.12

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ACS Style

Endang Nurcahyani; R. Agustrina; T. T. Handayani. The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. J. Plant Sci. 2016, 4(5), 102-105. doi: 10.11648/j.jps.20160405.12

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AMA Style

Endang Nurcahyani, R. Agustrina, T. T. Handayani. The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. J Plant Sci. 2016;4(5):102-105. doi: 10.11648/j.jps.20160405.12

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@article{10.11648/j.jps.20160405.12,
  author = {Endang Nurcahyani and R. Agustrina and T. T. Handayani},
  title = {The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum},
  journal = {Journal of Plant Sciences},
  volume = {4},
  number = {5},
  pages = {102-105},
  doi = {10.11648/j.jps.20160405.12},
  url = {https://doi.org/10.11648/j.jps.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160405.12},
  abstract = {Ground orchid (Spathoglottis plicata) cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum (Fo). A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin and Went (VW) medium containing fusaric acid (FA) at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm), and there were indications of FA tolerant of the selected plantlets. This study aims to describe the formation mechanism of Induced Resistance by analysing the protein profile of the plantlet. The protein profile was analysed by SDS-PAGE methods. The results showed that protein profiles of explants leaves was significantly different from the control. There was an initiation of a new protein bands (± 19 kD) indicating the formation of PR-protein (peroxidase) on the plantlets which responsible for S. plicata resistant to Fo.},
 year = {2016}
}

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TY  - JOUR
T1  - The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum
AU  - Endang Nurcahyani
AU  - R. Agustrina
AU  - T. T. Handayani
Y1  - 2016/08/21
PY  - 2016
N1  - https://doi.org/10.11648/j.jps.20160405.12
DO  - 10.11648/j.jps.20160405.12
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 102
EP  - 105
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20160405.12
AB  - Ground orchid (Spathoglottis plicata) cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum (Fo). A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin and Went (VW) medium containing fusaric acid (FA) at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm), and there were indications of FA tolerant of the selected plantlets. This study aims to describe the formation mechanism of Induced Resistance by analysing the protein profile of the plantlet. The protein profile was analysed by SDS-PAGE methods. The results showed that protein profiles of explants leaves was significantly different from the control. There was an initiation of a new protein bands (± 19 kD) indicating the formation of PR-protein (peroxidase) on the plantlets which responsible for S. plicata resistant to Fo.
VL  - 4
IS  - 5
ER  -

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Author Information

Endang Nurcahyani

Dept. of Biology, Faculty of Mathematics and Natural of Science, Lampung University, Bandar Lampung, Indonesia
R. Agustrina

Dept. of Biology, Faculty of Mathematics and Natural of Science, Lampung University, Bandar Lampung, Indonesia
T. T. Handayani

Dept. of Biology, Faculty of Mathematics and Natural of Science, Lampung University, Bandar Lampung, Indonesia

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Plain Text BibTeX RIS

APA Style

Endang Nurcahyani, R. Agustrina, T. T. Handayani. (2016). The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. Journal of Plant Sciences, 4(5), 102-105. https://doi.org/10.11648/j.jps.20160405.12

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ACS Style

Endang Nurcahyani; R. Agustrina; T. T. Handayani. The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. J. Plant Sci. 2016, 4(5), 102-105. doi: 10.11648/j.jps.20160405.12

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AMA Style

Endang Nurcahyani, R. Agustrina, T. T. Handayani. The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. J Plant Sci. 2016;4(5):102-105. doi: 10.11648/j.jps.20160405.12

Copy | Download

@article{10.11648/j.jps.20160405.12,
  author = {Endang Nurcahyani and R. Agustrina and T. T. Handayani},
  title = {The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum},
  journal = {Journal of Plant Sciences},
  volume = {4},
  number = {5},
  pages = {102-105},
  doi = {10.11648/j.jps.20160405.12},
  url = {https://doi.org/10.11648/j.jps.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160405.12},
  abstract = {Ground orchid (Spathoglottis plicata) cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum (Fo). A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin and Went (VW) medium containing fusaric acid (FA) at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm), and there were indications of FA tolerant of the selected plantlets. This study aims to describe the formation mechanism of Induced Resistance by analysing the protein profile of the plantlet. The protein profile was analysed by SDS-PAGE methods. The results showed that protein profiles of explants leaves was significantly different from the control. There was an initiation of a new protein bands (± 19 kD) indicating the formation of PR-protein (peroxidase) on the plantlets which responsible for S. plicata resistant to Fo.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum
AU  - Endang Nurcahyani
AU  - R. Agustrina
AU  - T. T. Handayani
Y1  - 2016/08/21
PY  - 2016
N1  - https://doi.org/10.11648/j.jps.20160405.12
DO  - 10.11648/j.jps.20160405.12
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 102
EP  - 105
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20160405.12
AB  - Ground orchid (Spathoglottis plicata) cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum (Fo). A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin and Went (VW) medium containing fusaric acid (FA) at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm), and there were indications of FA tolerant of the selected plantlets. This study aims to describe the formation mechanism of Induced Resistance by analysing the protein profile of the plantlet. The protein profile was analysed by SDS-PAGE methods. The results showed that protein profiles of explants leaves was significantly different from the control. There was an initiation of a new protein bands (± 19 kD) indicating the formation of PR-protein (peroxidase) on the plantlets which responsible for S. plicata resistant to Fo.
VL  - 4
IS  - 5
ER  -

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