Plants exposed to high temperature causes inhibition of photosynthesis, cell membrane damage and cell death. Plants have a number of protective systems against deleterious stress effects through the synthesis of proteins and enzymes. This study was performed to investigate the effects of heat stress on synthesis of proteins and reactive oxygen species scavenging enzymes in fenugreek (Trigonella foenum-graceum) plants. The plants were exposed to 30 ºC, 35 ºC and 40 ºC for 4 hrs and 6 hrs to induce heat stress. Catalase and peroxidase assays were performed to determine the defense mechanisms by T. foenum-graceum plants against reactive oxygen species produced due to heat stress. There was an increased enzymatic activity in response to exposure time and temperature. Proteins were extracted from heat stressed plants and SDS-PAGE analysis revealed the variation among the protein bands and high level of gene expression results in synthesis of proteins having molecular weight of 30 - 60 kDa. MALDI-TOF-MS was used to compare the peptide mass fingerprinting (PMF) data with SWISS-PORT and NCBI database for the identification of expressed proteins in response to heat stress.
| Published in | Journal of Plant Sciences (Volume 1, Issue 4) |
| DOI | 10.11648/j.jps.20130104.11 |
| Page(s) | 51-56 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2013. Published by Science Publishing Group |
Heat Shock Proteins, Heat Stress, Reactive Oxygen Species, Trigonella
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APA Style
Geetika Pant, Sanivarapu Hemalatha, Selvam Arjunan, Sudhakar Malla, G. Sibi. (2013). Effect of Heat Stress in Synthesis of Heat Shock Proteins and Antioxidative Enzyme Response in Trigonella foenum-graceum L. Journal of Plant Sciences, 1(4), 51-56. https://doi.org/10.11648/j.jps.20130104.11
ACS Style
Geetika Pant; Sanivarapu Hemalatha; Selvam Arjunan; Sudhakar Malla; G. Sibi. Effect of Heat Stress in Synthesis of Heat Shock Proteins and Antioxidative Enzyme Response in Trigonella foenum-graceum L. J. Plant Sci. 2013, 1(4), 51-56. doi: 10.11648/j.jps.20130104.11
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Download@article{10.11648/j.jps.20130104.11,
author = {Geetika Pant and Sanivarapu Hemalatha and Selvam Arjunan and Sudhakar Malla and G. Sibi},
title = {Effect of Heat Stress in Synthesis of Heat Shock Proteins and Antioxidative Enzyme Response in Trigonella foenum-graceum L},
journal = {Journal of Plant Sciences},
volume = {1},
number = {4},
pages = {51-56},
doi = {10.11648/j.jps.20130104.11},
url = {https://doi.org/10.11648/j.jps.20130104.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20130104.11},
abstract = {Plants exposed to high temperature causes inhibition of photosynthesis, cell membrane damage and cell death. Plants have a number of protective systems against deleterious stress effects through the synthesis of proteins and enzymes. This study was performed to investigate the effects of heat stress on synthesis of proteins and reactive oxygen species scavenging enzymes in fenugreek (Trigonella foenum-graceum) plants. The plants were exposed to 30 ºC, 35 ºC and 40 ºC for 4 hrs and 6 hrs to induce heat stress. Catalase and peroxidase assays were performed to determine the defense mechanisms by T. foenum-graceum plants against reactive oxygen species produced due to heat stress. There was an increased enzymatic activity in response to exposure time and temperature. Proteins were extracted from heat stressed plants and SDS-PAGE analysis revealed the variation among the protein bands and high level of gene expression results in synthesis of proteins having molecular weight of 30 - 60 kDa. MALDI-TOF-MS was used to compare the peptide mass fingerprinting (PMF) data with SWISS-PORT and NCBI database for the identification of expressed proteins in response to heat stress.},
year = {2013}
}
TY - JOUR T1 - Effect of Heat Stress in Synthesis of Heat Shock Proteins and Antioxidative Enzyme Response in Trigonella foenum-graceum L AU - Geetika Pant AU - Sanivarapu Hemalatha AU - Selvam Arjunan AU - Sudhakar Malla AU - G. Sibi Y1 - 2013/11/30 PY - 2013 N1 - https://doi.org/10.11648/j.jps.20130104.11 DO - 10.11648/j.jps.20130104.11 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 51 EP - 56 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20130104.11 AB - Plants exposed to high temperature causes inhibition of photosynthesis, cell membrane damage and cell death. Plants have a number of protective systems against deleterious stress effects through the synthesis of proteins and enzymes. This study was performed to investigate the effects of heat stress on synthesis of proteins and reactive oxygen species scavenging enzymes in fenugreek (Trigonella foenum-graceum) plants. The plants were exposed to 30 ºC, 35 ºC and 40 ºC for 4 hrs and 6 hrs to induce heat stress. Catalase and peroxidase assays were performed to determine the defense mechanisms by T. foenum-graceum plants against reactive oxygen species produced due to heat stress. There was an increased enzymatic activity in response to exposure time and temperature. Proteins were extracted from heat stressed plants and SDS-PAGE analysis revealed the variation among the protein bands and high level of gene expression results in synthesis of proteins having molecular weight of 30 - 60 kDa. MALDI-TOF-MS was used to compare the peptide mass fingerprinting (PMF) data with SWISS-PORT and NCBI database for the identification of expressed proteins in response to heat stress. VL - 1 IS - 4 ER -
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