Choreospondias axillaries, locally called Lapsi is a dioecous, deciduous fruit bearing plant, with multiple daily uses. The protease was extracted with 0.1M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. Thus obtained ammonium sulfate precipitated fraction of protease was characterized. The protease from root showed maximum activity at pH 10, temperature at 35°C. The optimum time of incubation was 15 minute. A bell shaped curve was obtained for the effect of enzyme concentration with optimum enzyme 50µg. The Km and Vmax value of root were 1.53nM and 52.91pmol/min.
| Published in | Journal of Plant Sciences (Volume 1, Issue 3) |
| DOI | 10.11648/j.jps.20130103.14 |
| Page(s) | 39-42 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2013. Published by Science Publishing Group |
Protease, Lapsi, Purification, Characterization, Isolation
| [1] | Nguyend. D., N. H. Nguyen, T. T. Nguyen, T. S. Phan, Vand. Nguyen, M. Grabe, R. Johansson, G. Lind-gren, N. E. Stjernsrom and T. A. Soderberg (1996): The use of water extracts from the root of Choreospondias axillaris the treatment of second degree burns. Scand J Plast Reconstr Hand Surg 30, 139–144. |
| [2] | Shah, D. J. (1978): Ascorbic acid (Vitamin C) content of Lapsi-pulp and peel at different stage of maturation. Research Bulletin 2035 B. S. Food Research Section, HMGN, Dept. of Food and Agricultural Marketing Ser-vices, Kathmandu. |
| [3] | Agrawal, G. K., Khandka D. K., Jha B., Thapa A., Tamrakar S., Manandhar S. P., Chaudhary. P. and Agrawal V. P. (1991): Studies on methods for early sex determination of Choreospondias axillaris a fruit bearing Himalayan tree. Biotechnology Letters (Nepal) 1, 23–28. |
| [4] | Agrawal V.P., Keshari A and Singh D (1992): Study of Lapsi (Choreospondias axillaris) Protease Unpublished result. |
| [5] | Dekhang R.N. and Sharma G (2006): Study of Protease from the leaves of Choreospondias axillaris (Lapsi). Submitted to Universal Science College, Biochemistry Department for the fulfillment of degree requirement of B.Sc. Biochemistry. |
| [6] | Singh R and Giri S (2007): Characterization and purification of protease from leaves of Choreospondias axillaris (Lapsi).Submitted to Universal Science College, Biochemistry Department for the fulfillment of degree requirement of B.Sc. Biochemistry. |
| [7] | McPhie, P. (1971): Dialysis. Meth. Enzymol. 22, 23-33. |
| [8] | Bradford M.M. (1976): A Rapid and Sensitive Method for the Quantization of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry 72: 248-254. |
| [9] | Hilger C, Grigioni F, De Beaufort C, Michel G, Freilinger J, and Hentger F (2001): Differential binding of IgG and IgA antibodies to antigenic determinants of bovine serum albumin. J. Clin. Exp. Immupol. 123 (3), 387-394. |
| [10] | Lineweaver H, Burk D (1934): The determination of enzyme dissociation constants. J.Amer. Chem. Soc. 56:658. |
APA Style
Shrawan Kumar Upadhyay, Rubin Thapa Magar, Chandan Jung Thapa. (2013). Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi). Journal of Plant Sciences, 1(3), 39-42. https://doi.org/10.11648/j.jps.20130103.14
ACS Style
Shrawan Kumar Upadhyay; Rubin Thapa Magar; Chandan Jung Thapa. Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi). J. Plant Sci. 2013, 1(3), 39-42. doi: 10.11648/j.jps.20130103.14
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.jps.20130103.14,
author = {Shrawan Kumar Upadhyay and Rubin Thapa Magar and Chandan Jung Thapa},
title = {Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi)},
journal = {Journal of Plant Sciences},
volume = {1},
number = {3},
pages = {39-42},
doi = {10.11648/j.jps.20130103.14},
url = {https://doi.org/10.11648/j.jps.20130103.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20130103.14},
abstract = {Choreospondias axillaries, locally called Lapsi is a dioecous, deciduous fruit bearing plant, with multiple daily uses. The protease was extracted with 0.1M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. Thus obtained ammonium sulfate precipitated fraction of protease was characterized. The protease from root showed maximum activity at pH 10, temperature at 35°C. The optimum time of incubation was 15 minute. A bell shaped curve was obtained for the effect of enzyme concentration with optimum enzyme 50µg. The Km and Vmax value of root were 1.53nM and 52.91pmol/min.},
year = {2013}
}
TY - JOUR T1 - Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi) AU - Shrawan Kumar Upadhyay AU - Rubin Thapa Magar AU - Chandan Jung Thapa Y1 - 2013/11/10 PY - 2013 N1 - https://doi.org/10.11648/j.jps.20130103.14 DO - 10.11648/j.jps.20130103.14 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 39 EP - 42 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20130103.14 AB - Choreospondias axillaries, locally called Lapsi is a dioecous, deciduous fruit bearing plant, with multiple daily uses. The protease was extracted with 0.1M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. Thus obtained ammonium sulfate precipitated fraction of protease was characterized. The protease from root showed maximum activity at pH 10, temperature at 35°C. The optimum time of incubation was 15 minute. A bell shaped curve was obtained for the effect of enzyme concentration with optimum enzyme 50µg. The Km and Vmax value of root were 1.53nM and 52.91pmol/min. VL - 1 IS - 3 ER -
Email: