Isolation, Purification and Structural Characterization of a Polysaccharide from <i>Rheum Palmatum</i> L.

Jinhua Li; Sihao Zheng; Weiguang Ren; Labin Wu; Linfang Huang

doi:doi:10.11648/j.jfns.20170503.13

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Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.

Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang

Published in Journal of Food and Nutrition Sciences (Volume 5, Issue 3)

Received: 14 February 2017 Accepted: 22 March 2017 Published: 14 April 2017

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Abstract

Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 10⁴ Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.

Published in	Journal of Food and Nutrition Sciences (Volume 5, Issue 3)
DOI	10.11648/j.jfns.20170503.13
Page(s)	73-78
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Rheum Palmatum L., Polysaccharide, Isolation, Purification, Structural Characterization

References

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APA Style

Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang. (2017). Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. Journal of Food and Nutrition Sciences, 5(3), 73-78. https://doi.org/10.11648/j.jfns.20170503.13

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ACS Style

Jinhua Li; Sihao Zheng; Weiguang Ren; Labin Wu; Linfang Huang. Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. J. Food Nutr. Sci. 2017, 5(3), 73-78. doi: 10.11648/j.jfns.20170503.13

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AMA Style

Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang. Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. J Food Nutr Sci. 2017;5(3):73-78. doi: 10.11648/j.jfns.20170503.13

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@article{10.11648/j.jfns.20170503.13,
  author = {Jinhua Li and Sihao Zheng and Weiguang Ren and Labin Wu and Linfang Huang},
  title = {Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {5},
  number = {3},
  pages = {73-78},
  doi = {10.11648/j.jfns.20170503.13},
  url = {https://doi.org/10.11648/j.jfns.20170503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20170503.13},
  abstract = {Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.},
 year = {2017}
}

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TY  - JOUR
T1  - Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.
AU  - Jinhua Li
AU  - Sihao Zheng
AU  - Weiguang Ren
AU  - Labin Wu
AU  - Linfang Huang
Y1  - 2017/04/14
PY  - 2017
N1  - https://doi.org/10.11648/j.jfns.20170503.13
DO  - 10.11648/j.jfns.20170503.13
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 73
EP  - 78
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20170503.13
AB  - Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.
VL  - 5
IS  - 3
ER  -

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Author Information

Jinhua Li

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
Sihao Zheng

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
Weiguang Ren

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
Labin Wu

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
Linfang Huang

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

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Plain Text BibTeX RIS

APA Style

Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang. (2017). Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. Journal of Food and Nutrition Sciences, 5(3), 73-78. https://doi.org/10.11648/j.jfns.20170503.13

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ACS Style

Jinhua Li; Sihao Zheng; Weiguang Ren; Labin Wu; Linfang Huang. Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. J. Food Nutr. Sci. 2017, 5(3), 73-78. doi: 10.11648/j.jfns.20170503.13

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AMA Style

Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang. Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. J Food Nutr Sci. 2017;5(3):73-78. doi: 10.11648/j.jfns.20170503.13

Copy | Download

@article{10.11648/j.jfns.20170503.13,
  author = {Jinhua Li and Sihao Zheng and Weiguang Ren and Labin Wu and Linfang Huang},
  title = {Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {5},
  number = {3},
  pages = {73-78},
  doi = {10.11648/j.jfns.20170503.13},
  url = {https://doi.org/10.11648/j.jfns.20170503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20170503.13},
  abstract = {Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.
AU  - Jinhua Li
AU  - Sihao Zheng
AU  - Weiguang Ren
AU  - Labin Wu
AU  - Linfang Huang
Y1  - 2017/04/14
PY  - 2017
N1  - https://doi.org/10.11648/j.jfns.20170503.13
DO  - 10.11648/j.jfns.20170503.13
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 73
EP  - 78
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20170503.13
AB  - Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.
VL  - 5
IS  - 3
ER  -

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