@article{10.11648/j.jdmp.20210701.13,
  author = {Helene Gueaba Mbuntcha and Hervet Paulin Dogmo Fogang and Verlaine Woguem and Patrick Sonkoue and Pamela Kenfack and Kelly Djeuga and Christine Schippa and Elisabet Dunach and Jackson Armel Seukep and Stephen Tamekou Lacmata and Theophile Fonkou and Leon Azefack Tapondjou and Hilaire Macaire Womeni},
  title = {Chemical Characterization and Antibacterial Efficacy of Essential Oils of Three Lamiaceae Species Growing in Cameroon},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {1},
  pages = {14-21},
  doi = {10.11648/j.jdmp.20210701.13},
  url = {https://doi.org/10.11648/j.jdmp.20210701.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210701.13},
  abstract = {Essential oils (EOs) have aroused attention among the naturally-occurring therapeutic compounds with anti-infective properties. This study examined the chemical composition and the antibacterial potential of EOs from three Lamiaceae species including Ocimum gratissimum (leaves), Plecthrantus epilithicus (leaves), and Satureja robusta (leaves and flowers). EOs were obtained by hydrodistillation using a Clevenger-type apparatus, followed by characterization by gas chromatography-mass spectrometry (GC-MS) and flame ionization detector (GC/FID). The antibacterial efficacy of EOs was screened using the microdilution method, against a panel of eight foodborne bacteria isolates namely Enterobacter cloacae, Yersinia enterocolotica, Klebsiella pneumoniae, Salmonella typhi, Escherichia coli, Citrobacter freundii, Shigella flexneri, and Enterococcus faecalis. GC-MS and GC/FID analysis led to the identification of 53 components from EOs of P. epilithicus, while around forty compounds have been characterized from the EOs of O. gratissimum (43), leaves of Satureja robusta (43), and flowers of S. robusta (44). The nature of identified compounds varied according to the species. The most prevalent volatile compounds identified in the EOs of P. epilithicus were piperitenone oxide (23.65%) and two isomers piperitone oxide 2/2 (16.15%) and piperitone oxide 1/2 (7.24%). The major constituents in the EOs of leaves of O. gratissimum were elemicin (33.474%) and eugenol (30.258%). Piperitone oxide, piperitone, and germacrene D were identified as major constituents in both the EOs of leaves of S. robusta (28.3%, 15.14%, and 9.42% respectively) and flowers (45.6%, 11.55%, and 3.94% respectively). The oils displayed selective antibacterial potential, with the recorded minimal inhibitory concentration (MIC) values ranging from 0.0156 to 1% (v/v). EOs of P. epilithicus as well as that of the leaves and flowers of S. robusta acted against all studied bacteria. Most of the MIC values were below 0.25% (v/v), indicating a strong inhibitory potential of studied EOs. The present study provides a strong baseline for consideration of the EOs from O. gratissimum, S. robusta, and P. epilithicus in the control of bacterial foodborne infections.},
 year = {2021}
}

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TY  - JOUR
T1  - Chemical Characterization and Antibacterial Efficacy of Essential Oils of Three Lamiaceae Species Growing in Cameroon
AU  - Helene Gueaba Mbuntcha
AU  - Hervet Paulin Dogmo Fogang
AU  - Verlaine Woguem
AU  - Patrick Sonkoue
AU  - Pamela Kenfack
AU  - Kelly Djeuga
AU  - Christine Schippa
AU  - Elisabet Dunach
AU  - Jackson Armel Seukep
AU  - Stephen Tamekou Lacmata
AU  - Theophile Fonkou
AU  - Leon Azefack Tapondjou
AU  - Hilaire Macaire Womeni
Y1  - 2021/02/27
PY  - 2021
N1  - https://doi.org/10.11648/j.jdmp.20210701.13
DO  - 10.11648/j.jdmp.20210701.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 14
EP  - 21
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20210701.13
AB  - Essential oils (EOs) have aroused attention among the naturally-occurring therapeutic compounds with anti-infective properties. This study examined the chemical composition and the antibacterial potential of EOs from three Lamiaceae species including Ocimum gratissimum (leaves), Plecthrantus epilithicus (leaves), and Satureja robusta (leaves and flowers). EOs were obtained by hydrodistillation using a Clevenger-type apparatus, followed by characterization by gas chromatography-mass spectrometry (GC-MS) and flame ionization detector (GC/FID). The antibacterial efficacy of EOs was screened using the microdilution method, against a panel of eight foodborne bacteria isolates namely Enterobacter cloacae, Yersinia enterocolotica, Klebsiella pneumoniae, Salmonella typhi, Escherichia coli, Citrobacter freundii, Shigella flexneri, and Enterococcus faecalis. GC-MS and GC/FID analysis led to the identification of 53 components from EOs of P. epilithicus, while around forty compounds have been characterized from the EOs of O. gratissimum (43), leaves of Satureja robusta (43), and flowers of S. robusta (44). The nature of identified compounds varied according to the species. The most prevalent volatile compounds identified in the EOs of P. epilithicus were piperitenone oxide (23.65%) and two isomers piperitone oxide 2/2 (16.15%) and piperitone oxide 1/2 (7.24%). The major constituents in the EOs of leaves of O. gratissimum were elemicin (33.474%) and eugenol (30.258%). Piperitone oxide, piperitone, and germacrene D were identified as major constituents in both the EOs of leaves of S. robusta (28.3%, 15.14%, and 9.42% respectively) and flowers (45.6%, 11.55%, and 3.94% respectively). The oils displayed selective antibacterial potential, with the recorded minimal inhibitory concentration (MIC) values ranging from 0.0156 to 1% (v/v). EOs of P. epilithicus as well as that of the leaves and flowers of S. robusta acted against all studied bacteria. Most of the MIC values were below 0.25% (v/v), indicating a strong inhibitory potential of studied EOs. The present study provides a strong baseline for consideration of the EOs from O. gratissimum, S. robusta, and P. epilithicus in the control of bacterial foodborne infections.
VL  - 7
IS  - 1
ER  - 

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