In Vitro Therapeutic Potential of Leaf Extract of <i>Eugenia uniflora linn</i> on Acute – inflammation Rat Model

Elizabeth Finbarrs Bello; Abireh Ifeanacho Ezeteonu; Ude Vincent

doi:doi:10.11648/j.jdmp.20200602.11

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In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model

Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent

Published in Journal of Diseases and Medicinal Plants (Volume 6, Issue 2)

Received: 27 February 2020 Accepted: 18 March 2020 Published: 12 May 2020

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Abstract

The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (P<0.05) decrease in the number of abdominal writhing and insignificant reduction in the second or late phases of formalin-induced paw licking test group. Reduction in RBC, HB, PCV and increase in platelet, ESR, WBC were observed in the formalin-induced paw edema group. Histopathological assessment revealed aggregation of inflammatory cells in skin sections of the untreated group of the formalin-induced paw edema but a noticeable reversal in the extract and Ibuprofen treatment groups. These profiles indicate E. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.

Published in	Journal of Diseases and Medicinal Plants (Volume 6, Issue 2)
DOI	10.11648/j.jdmp.20200602.11
Page(s)	31-38
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Edema, Inflammation, Analgesic, E. uniflora, Ibuprofen, Formalin

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APA Style

Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent. (2020). In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. Journal of Diseases and Medicinal Plants, 6(2), 31-38. https://doi.org/10.11648/j.jdmp.20200602.11

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ACS Style

Elizabeth Finbarrs Bello; Abireh Ifeanacho Ezeteonu; Ude Vincent. In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. J. Dis. Med. Plants 2020, 6(2), 31-38. doi: 10.11648/j.jdmp.20200602.11

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AMA Style

Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent. In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. J Dis Med Plants. 2020;6(2):31-38. doi: 10.11648/j.jdmp.20200602.11

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@article{10.11648/j.jdmp.20200602.11,
  author = {Elizabeth Finbarrs Bello and Abireh Ifeanacho Ezeteonu and Ude Vincent},
  title = {In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {6},
  number = {2},
  pages = {31-38},
  doi = {10.11648/j.jdmp.20200602.11},
  url = {https://doi.org/10.11648/j.jdmp.20200602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20200602.11},
  abstract = {The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (PE. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.},
 year = {2020}
}

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TY - JOUR
T1 - In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model
AU - Elizabeth Finbarrs Bello
AU - Abireh Ifeanacho Ezeteonu
AU - Ude Vincent
Y1 - 2020/05/12
PY - 2020
N1 - https://doi.org/10.11648/j.jdmp.20200602.11
DO - 10.11648/j.jdmp.20200602.11
T2 - Journal of Diseases and Medicinal Plants
JF - Journal of Diseases and Medicinal Plants
JO - Journal of Diseases and Medicinal Plants
SP - 31
EP - 38
PB - Science Publishing Group
SN - 2469-8210
UR - https://doi.org/10.11648/j.jdmp.20200602.11
AB - The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (PE. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.
VL - 6
IS - 2
ER -

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Author Information

Elizabeth Finbarrs Bello

Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria
Abireh Ifeanacho Ezeteonu

Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria
Ude Vincent

Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria

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Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent. (2020). In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. Journal of Diseases and Medicinal Plants, 6(2), 31-38. https://doi.org/10.11648/j.jdmp.20200602.11

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ACS Style

Elizabeth Finbarrs Bello; Abireh Ifeanacho Ezeteonu; Ude Vincent. In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. J. Dis. Med. Plants 2020, 6(2), 31-38. doi: 10.11648/j.jdmp.20200602.11

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AMA Style

Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent. In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model. J Dis Med Plants. 2020;6(2):31-38. doi: 10.11648/j.jdmp.20200602.11

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@article{10.11648/j.jdmp.20200602.11,
  author = {Elizabeth Finbarrs Bello and Abireh Ifeanacho Ezeteonu and Ude Vincent},
  title = {In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {6},
  number = {2},
  pages = {31-38},
  doi = {10.11648/j.jdmp.20200602.11},
  url = {https://doi.org/10.11648/j.jdmp.20200602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20200602.11},
  abstract = {The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (PE. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.},
 year = {2020}
}

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