Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste

Md. Fakruddin; Md. Ariful Islam; Monzur Morshed Ahmed; Nayuum Chowdhury

doi:doi:10.11648/j.ijrse.20130204.11

| Peer-Reviewed

Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste

Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury

Published in International Journal of Renewable and Sustainable Energy (Volume 2, Issue 4)

Received: 15 May 2013 Published: 10 July 2013

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.

Published in	International Journal of Renewable and Sustainable Energy (Volume 2, Issue 4)
DOI	10.11648/j.ijrse.20130204.11
Page(s)	133-139
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Ethanol, Molassesand Stress Tolerant

References

[1]	Jones, A.M., K.C. Thomas and W.M. Inglew, Ethanolic fermentation of molasses and sugarcane juice using very high gravity technology. Journal of Agricultural Chemistry, 1994. 42: p. 1242-12 46.
[2]	Isaias, M., V. Leal, L.M. Ramos and J.A. Da-Silva, Assessment of greenhouse gas emissions in the production and use of fuel ethanol in Brazil.Secretariat of the Environment, Government of the State of Sao Paulo, 2004.
[3]	Goettemoeller, J. and A. Goettemoeller, Sustainable Ethanol: Biofuels, Biorefineries, Cellulosic Biomass, Flex-Fuel Vehicles, and Sustainable Farming for Energy Independence. Praire Oak Publishing, Maryville, Missouri, ISBN: 9780978629304, 2007. p. 42.
[4]	Chandel, A.K., E.S. Chan, R. Rudravaram, M.L. Narasu, L.V. Rao and P. Ravindra, Economics and Enviromental Impact of Bioethanol Production Technologies: an Appraisal. Biotechnology and Molecular Biology Review, 2007. 2(1): p. 14-32.
[5]	Tsuyoshi, N., R. Fudou, S. Yamanaka, M. Kozaki, N. Tamang, S. Thapa, J.P. Tamang, Identification of yeast strains isolated from marcha in Sikkim, a microbial starter for amylolytic fermentation.International Journal of Food Microbiology, 2005. 9(2): p. 135-146.
[6]	Osunkoya, O.A. and N.J. Okwudinka, Utilization of sugar refinery waste (molasses) for ethanol productionusing Saccharomyces cerevisiae.American Journal of Scientific and Industrial Research, 2011. 2(4): p. 694-706.
[7]	Somda, M.K., A. Savadogo, C.A.T. Ouattara, A.S. Ouattara and A.S. Traore, Thermotolerant and Alcohol-tolerant yeasts targeted to optimize hydrolyzation from mango peel for high bioethanol production. Asian Journal of Biotechnology, 2011. 3 (1): p. 77-83.
[8]	Khongsay, N., L. Laopaiboon and P. Laopaiboon, Growth and Batch fermentation of Saccharomyces cerevisiae on sweet sorghum stem juice under normal and very high gravity conditions. Biotechnology, 2010. 9(1): p. 9-16.
[9]	Boboye, B. andI. Dayo-Owoyemi, Evaluation of Dough Sensory Properties Impacted by Yeasts Isolated from Cassava. Journal of Applied Sciences, 2009. 9(4): p. 771-776.
[10]	Fakruddin, M., M.A. Quayum, M.M. Ahmed, N. Chowdhury, Analysis of Key Factors Affecting Ethanol Production by Saccharomyces cerevisiae IFST-072011. Biotechnology, 2012. 11(4): p. 248-252.
[11]	Miller, G.L., Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Analytical Chemistry, 1959. 31: p. 426-28.
[12]	Conway, E.J., Microdiffusion analysis and volumetric error. Crosby Lockwood and Son, London, 1939.
[13]	Lodder, J., The yeasts: A Taxonomic study. NorthHoll and Publishing, Amsterdam, 1971.
[14]	Boekhout, T. and C.P. Kurtzman, Principles and methods used in yeast classification, and an overview of currently accepted yeast genera. In Wolf, K. Nonconventional Yeasts in Biotechnology: A Handbook. Springer-Verlag: Heidelberg. 1996. p. 1-99
[15]	Mariam, I., K. Manzoor, S. Ali and Ikram-Ul-Haq, Enhanced production of from free and immobilized Saccharomyces cerevisiaeunder stationary culture. Pakistan Journal of Botany, 2009. 41: p. 821823.
[16]	Arshad, M., M.A. Zia, M. Asghar and H. Bhatti, (2011). Improving bio-ethanol yield: Using virginiamycin and sodium flouride at a Pakistani distillery. African Journal of Biotechnology, 2011. 10(53): p. 11071-11074.
[17]	Willaert, R. and A.N. Viktor, Primay beer fermentation by immobilized yeast - a review on flavor formation and control strategies. Journal of Chemical Technology & Biotechnology, 2006. 81: p. 1353-1367.
[18]	Walker, G.M. and P.V. Dijck, Physiological and molecular responses of yeasts to the environment, p. 111-152. In Querol, A. and G. Fleet (eds.), Yeasts in food and beverages, Springer-Verlag Berlin, Germany, 2006.

Cite This Article

Plain Text BibTeX RIS

APA Style

Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury. (2013). Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. International Journal of Sustainable and Green Energy, 2(4), 133-139. https://doi.org/10.11648/j.ijrse.20130204.11

Copy | Download

ACS Style

Md. Fakruddin; Md. Ariful Islam; Monzur Morshed Ahmed; Nayuum Chowdhury. Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int. J. Sustain. Green Energy 2013, 2(4), 133-139. doi: 10.11648/j.ijrse.20130204.11

Copy | Download

AMA Style

Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury. Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int J Sustain Green Energy. 2013;2(4):133-139. doi: 10.11648/j.ijrse.20130204.11

Copy | Download

@article{10.11648/j.ijrse.20130204.11,
  author = {Md. Fakruddin and Md. Ariful Islam and Monzur Morshed Ahmed and Nayuum Chowdhury},
  title = {Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste},
  journal = {International Journal of Sustainable and Green Energy},
  volume = {2},
  number = {4},
  pages = {133-139},
  doi = {10.11648/j.ijrse.20130204.11},
  url = {https://doi.org/10.11648/j.ijrse.20130204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.20130204.11},
  abstract = {The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste
AU  - Md. Fakruddin
AU  - Md. Ariful Islam
AU  - Monzur Morshed Ahmed
AU  - Nayuum Chowdhury
Y1  - 2013/07/10
PY  - 2013
N1  - https://doi.org/10.11648/j.ijrse.20130204.11
DO  - 10.11648/j.ijrse.20130204.11
T2  - International Journal of Sustainable and Green Energy
JF  - International Journal of Sustainable and Green Energy
JO  - International Journal of Sustainable and Green Energy
SP  - 133
EP  - 139
PB  - Science Publishing Group
SN  - 2575-1549
UR  - https://doi.org/10.11648/j.ijrse.20130204.11
AB  - The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.
VL  - 2
IS  - 4
ER  -

Copy | Download

Author Information

Md. Fakruddin

Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial research (BCSIR), Dhaka, Bangladesh
Md. Ariful Islam

Departments of Biotechnology, BRAC University, Dhaka, Bangladesh
Monzur Morshed Ahmed

Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial research (BCSIR), Dhaka, Bangladesh
Nayuum Chowdhury

Departments of Biotechnology, BRAC University, Dhaka, Bangladesh

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury. (2013). Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. International Journal of Sustainable and Green Energy, 2(4), 133-139. https://doi.org/10.11648/j.ijrse.20130204.11

Copy | Download

ACS Style

Md. Fakruddin; Md. Ariful Islam; Monzur Morshed Ahmed; Nayuum Chowdhury. Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int. J. Sustain. Green Energy 2013, 2(4), 133-139. doi: 10.11648/j.ijrse.20130204.11

Copy | Download

AMA Style

Md. Fakruddin, Md. Ariful Islam, Monzur Morshed Ahmed, Nayuum Chowdhury. Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste. Int J Sustain Green Energy. 2013;2(4):133-139. doi: 10.11648/j.ijrse.20130204.11

Copy | Download

@article{10.11648/j.ijrse.20130204.11,
  author = {Md. Fakruddin and Md. Ariful Islam and Monzur Morshed Ahmed and Nayuum Chowdhury},
  title = {Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste},
  journal = {International Journal of Sustainable and Green Energy},
  volume = {2},
  number = {4},
  pages = {133-139},
  doi = {10.11648/j.ijrse.20130204.11},
  url = {https://doi.org/10.11648/j.ijrse.20130204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.20130204.11},
  abstract = {The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Process Optimization of Bioethanol Production by Stress Tolerant Yeasts Isolated From Agro-Industrial Waste
AU  - Md. Fakruddin
AU  - Md. Ariful Islam
AU  - Monzur Morshed Ahmed
AU  - Nayuum Chowdhury
Y1  - 2013/07/10
PY  - 2013
N1  - https://doi.org/10.11648/j.ijrse.20130204.11
DO  - 10.11648/j.ijrse.20130204.11
T2  - International Journal of Sustainable and Green Energy
JF  - International Journal of Sustainable and Green Energy
JO  - International Journal of Sustainable and Green Energy
SP  - 133
EP  - 139
PB  - Science Publishing Group
SN  - 2575-1549
UR  - https://doi.org/10.11648/j.ijrse.20130204.11
AB  - The need of Bioethanol or biofuel is increasing worldwide day by day due to renewable resources and ease of production form cheap raw materials. There are several factors that affect on bioethanol production by using yeast. The main objective of this research work was to isolate stress tolerant yeast from agro industry and optimize a process for ethanol production by considering all the factors. Several fermentation batches were carried out by 3 stress tolerant strains varying temperature, pH, sugar concentration, aeration, immobilization and metal ions. From different experiments it was found that temperature 30°C, reducing sugar concentration ranged between 5-6%, pH between 5.0 - 6.0 and shaking condition were optimum for maximum yield of ethanol by strains Saccharomyces unisporous (P), Saccharomyces cerevisiae (C) and (T).The Bioethanol production capacity of yeasts were found P -15.00%, C -12.50% and T – 10.15%at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition (115 rpm).Pilot scale ethanol production by P strain was 13.10%, C strain 11.15% andT strain 9.80% at 60 hours. Immobilized cells were produced more ethanol than free cells with same culture conditions. Effect of potassium, magnesium, chromium and boron was investigated on ethanol production. Potassium, Magnesium was shown stimulatory effect on ethanol production.
VL  - 2
IS  - 4
ER  -

Copy | Download