Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction

Asim A. Osman; Ayman M. Mahmoud; Ayman S. Soliman

doi:doi:10.11648/j.ijnfs.20140303.12

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Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction

Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 3)

Received: 1 April 2014 Accepted: 20 April 2014 Published: 30 April 2014

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Abstract

This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.

Published in	International Journal of Nutrition and Food Sciences (Volume 3, Issue 3)
DOI	10.11648/j.ijnfs.20140303.12
Page(s)	141-144
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Cytochrome-b, Cow Milk, PCR

References

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APA Style

Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman. (2014). Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. International Journal of Nutrition and Food Sciences, 3(3), 141-144. https://doi.org/10.11648/j.ijnfs.20140303.12

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ACS Style

Asim A. Osman; Ayman M. Mahmoud; Ayman S. Soliman. Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. Int. J. Nutr. Food Sci. 2014, 3(3), 141-144. doi: 10.11648/j.ijnfs.20140303.12

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AMA Style

Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman. Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. Int J Nutr Food Sci. 2014;3(3):141-144. doi: 10.11648/j.ijnfs.20140303.12

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@article{10.11648/j.ijnfs.20140303.12,
  author = {Asim A. Osman and Ayman M. Mahmoud and Ayman S. Soliman},
  title = {Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {3},
  pages = {141-144},
  doi = {10.11648/j.ijnfs.20140303.12},
  url = {https://doi.org/10.11648/j.ijnfs.20140303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140303.12},
  abstract = {This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.},
 year = {2014}
}

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TY  - JOUR
T1  - Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction
AU  - Asim A. Osman
AU  - Ayman M. Mahmoud
AU  - Ayman S. Soliman
Y1  - 2014/04/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140303.12
DO  - 10.11648/j.ijnfs.20140303.12
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 141
EP  - 144
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140303.12
AB  - This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.
VL  - 3
IS  - 3
ER  -

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Author Information

Asim A. Osman

Physiology Department, Faculty of Medicine, Zawia University, Libya
Ayman M. Mahmoud

Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Egypt
Ayman S. Soliman

Physiology Department, Faculty of Medicine, Beni-Suef University, Egypt

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Plain Text BibTeX RIS

APA Style

Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman. (2014). Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. International Journal of Nutrition and Food Sciences, 3(3), 141-144. https://doi.org/10.11648/j.ijnfs.20140303.12

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ACS Style

Asim A. Osman; Ayman M. Mahmoud; Ayman S. Soliman. Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. Int. J. Nutr. Food Sci. 2014, 3(3), 141-144. doi: 10.11648/j.ijnfs.20140303.12

Copy | Download

AMA Style

Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman. Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. Int J Nutr Food Sci. 2014;3(3):141-144. doi: 10.11648/j.ijnfs.20140303.12

Copy | Download

@article{10.11648/j.ijnfs.20140303.12,
  author = {Asim A. Osman and Ayman M. Mahmoud and Ayman S. Soliman},
  title = {Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {3},
  pages = {141-144},
  doi = {10.11648/j.ijnfs.20140303.12},
  url = {https://doi.org/10.11648/j.ijnfs.20140303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140303.12},
  abstract = {This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction
AU  - Asim A. Osman
AU  - Ayman M. Mahmoud
AU  - Ayman S. Soliman
Y1  - 2014/04/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140303.12
DO  - 10.11648/j.ijnfs.20140303.12
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 141
EP  - 144
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140303.12
AB  - This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.
VL  - 3
IS  - 3
ER  -

Copy | Download