The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice

Miaoxuan Luo; Shanshan Wei; Xiangbin Mi; Tangde Zhang; Wu Zhang

doi:doi:10.11648/j.iji.20210903.11

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The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice

Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang

Published in International Journal of Immunology (Volume 9, Issue 3)

Received: 17 July 2021 Accepted: 9 August 2021 Published: 18 August 2021

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Abstract

Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4⁺T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4⁺T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (P<0.05); Meanwhile, down regulation of DNMT1 and DNA hypomethylation were found in MRL/lpr mice T cells. In contrary, the expression levels of CD70 increased when compared with healthy control (P<0.05). The same changes were seen in healthy CD4⁺T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.

Published in	International Journal of Immunology (Volume 9, Issue 3)
DOI	10.11648/j.iji.20210903.11
Page(s)	41-46
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Systemic Lupus Erythematosus, DNA Methylation, ERK1 Signal Pathway, Epigenetics

References

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APA Style

Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang. (2021). The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. International Journal of Immunology, 9(3), 41-46. https://doi.org/10.11648/j.iji.20210903.11

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ACS Style

Miaoxuan Luo; Shanshan Wei; Xiangbin Mi; Tangde Zhang; Wu Zhang. The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. Int. J. Immunol. 2021, 9(3), 41-46. doi: 10.11648/j.iji.20210903.11

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AMA Style

Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang. The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. Int J Immunol. 2021;9(3):41-46. doi: 10.11648/j.iji.20210903.11

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@article{10.11648/j.iji.20210903.11,
  author = {Miaoxuan Luo and Shanshan Wei and Xiangbin Mi and Tangde Zhang and Wu Zhang},
  title = {The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice},
  journal = {International Journal of Immunology},
  volume = {9},
  number = {3},
  pages = {41-46},
  doi = {10.11648/j.iji.20210903.11},
  url = {https://doi.org/10.11648/j.iji.20210903.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20210903.11},
  abstract = {Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (PP+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.},
 year = {2021}
}

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TY  - JOUR
T1  - The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice
AU  - Miaoxuan Luo
AU  - Shanshan Wei
AU  - Xiangbin Mi
AU  - Tangde Zhang
AU  - Wu Zhang
Y1  - 2021/08/18
PY  - 2021
N1  - https://doi.org/10.11648/j.iji.20210903.11
DO  - 10.11648/j.iji.20210903.11
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 41
EP  - 46
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20210903.11
AB  - Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (PP+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.
VL  - 9
IS  - 3
ER  -

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Author Information

Miaoxuan Luo

Department of Dermatology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Shanshan Wei

Department of Dermatology, Zhujiang Hospital, Southern Medical University/The Second School of Clinical Medicine, Guangzhou, China
Xiangbin Mi

Department of Dermatology, Zhujiang Hospital, Southern Medical University/The Second School of Clinical Medicine, Guangzhou, China
Tangde Zhang

Department of Dermatology, Zhujiang Hospital, Southern Medical University/The Second School of Clinical Medicine, Guangzhou, China
Wu Zhang

Department of Dermatology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

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APA Style

Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang. (2021). The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. International Journal of Immunology, 9(3), 41-46. https://doi.org/10.11648/j.iji.20210903.11

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ACS Style

Miaoxuan Luo; Shanshan Wei; Xiangbin Mi; Tangde Zhang; Wu Zhang. The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. Int. J. Immunol. 2021, 9(3), 41-46. doi: 10.11648/j.iji.20210903.11

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AMA Style

Miaoxuan Luo, Shanshan Wei, Xiangbin Mi, Tangde Zhang, Wu Zhang. The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice. Int J Immunol. 2021;9(3):41-46. doi: 10.11648/j.iji.20210903.11

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@article{10.11648/j.iji.20210903.11,
  author = {Miaoxuan Luo and Shanshan Wei and Xiangbin Mi and Tangde Zhang and Wu Zhang},
  title = {The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice},
  journal = {International Journal of Immunology},
  volume = {9},
  number = {3},
  pages = {41-46},
  doi = {10.11648/j.iji.20210903.11},
  url = {https://doi.org/10.11648/j.iji.20210903.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20210903.11},
  abstract = {Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (PP+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - The Regulatory Effect of ERK1 Pathway in the DNA Hypomethylation of MRL/lpr Mice
AU  - Miaoxuan Luo
AU  - Shanshan Wei
AU  - Xiangbin Mi
AU  - Tangde Zhang
AU  - Wu Zhang
Y1  - 2021/08/18
PY  - 2021
N1  - https://doi.org/10.11648/j.iji.20210903.11
DO  - 10.11648/j.iji.20210903.11
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 41
EP  - 46
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20210903.11
AB  - Background: Defective ERK1 activity may regulate the DNA hypomethylation in T cells of lupus mice. Aims: To investigate the function of ERK1 in regulating DNA hypomethylation and explore the potential molecular mechanisms involved in lupus mice. Methods: CD4+T cells were isolated from MRL/lpr and BALB/c mice, activated in vitro in the presence or absence of 5-azacytidine (5-Aza) (a DNA methyltransferase inhibitor)/U0126 (a selective inhibitor of the ERK signaling pathway) respectively. The positive rate of CD4+T cell was measured by flow cytometry, and expression levels of CD70, ERK1, DNA transferase 1 (DNMT1) were estimated by RT-PCR and Western blot. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. Results: The expression level of p-ERK1 in MRL/lpr mice was significantly lower than healthy control (PP+T cells treated with U0126. Conclusions: DNA hypomethylation regulating mechanisms in lupus T cells were associated with ERK1 signal pathway. U0126 could inhibit ERK1 signal transduction pathway in normal T cells, and induce DNA hypomethylation by regulating the expression of methylation sensitive gene CD70.
VL  - 9
IS  - 3
ER  -

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