The aim of investigation was the determination of the levels of IgE, IgG and complement activity in the blood and lymph in the experimental Arthus and Overy phenomenon. Experiments were carried out on 27 chinchilla rabbits (weight 2.5-3kg) in three series. To reproduce the Arthus phenomenon, rabbits were sensitized by subcutaneous administration of 1 ml of horse serum to the scapular region every 5 days; after the fifth injection in the injection area of horse serum the necrosis was observed. To obtain the Overy phenomenon, a resolving dose of horse serum was injected subcutaneously in a sensitized animal, and an Evans blue was introduced into the auricular vein. In this case, the injection area is colored blue. The blood necessary for the experiment was taken from the marginal auricular vein of the rabbit, and lymph – from the thoracic lymphatic duct. The levels of IgE and IgG and the activity of the complement comparatively in the blood and lymph in the experimental Arthus and Overy phenomenon were studied. The experiments were carried out on 27 rabbits of the «Chinchilla» breed in two series. The studied parameters of IgE and IgG and complement activity in blood and lymph of intact animals served as control. The results of the study showed that the level of IgG in the Arthus phenomenon increases and IgE decreases. While at the Overy phenomenon, on the contrary, the level of IgE increases and the level of IgG decreases. The titer of complement in immune complex reactions is sharply reduced; it indicates the involvement of complement with IgG elimination of the allergen.
| Published in | International Journal of Immunology (Volume 7, Issue 3) |
| DOI | 10.11648/j.iji.20190703.12 |
| Page(s) | 37-40 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Immunoglobulins, Arthus and Overy Phenomenon, Complement
| [1] | Babakhin A. A., Shilovsky I. P., Andreev I. V., Cozmin A. D. et al. Experimental allergen-specific immunotherapy of timpol allergo-vaccine on the model of IgE-dependent bronchial asthma in mice//Immunology, 2012, №3, pp. 134-142. |
| [2] | Gushin I. C. IgE-mediated hypersensitivity as a response to the barrier function of tissues//Immunology, 2015, Vol. 36, no. 1, pp. 45-52. |
| [3] | Konisheva A. Y., Samoylikov P. A., Shternshis Y. A., Gervazieva V. B. Determination of autoantibodies of antibodies to tissue antigens in patients with allergic diseases//Immunology, 2010, vol. 11.№3, p. 17-22. |
| [4] | Kuznik B. I. Coagulability and fibrinolytic activity of lymph in various pathological conditions//Pathological physiology and experimental therapy, 2013 №1, p. 39-42. |
| [5] | Ukhanova O. P. Study of the effect of monoclonal antibodies to human IgE on activation of peripheral blood basophils in patients with seasonal allergic rhinitis//Immunology, 2010, vol. 11.№1, p. 34-39. |
| [6] | Fedicheva N. A. Qorbov V. V. Features of cytokine balance and immunoglobulin E concentration in patients with chronic nephritis depending on the results of treatment//Medical immunology, 2010, T12, № 4-5, p. 89-91. |
| [7] | Shatokhin M. N., Konoplya A. I., Teodorovich O. V., Karaulov A. V. Cytokine status and complement system in chronic prostatitis. Pharmacological correction of violations//Immunology, 2013, №1, p. 156-159. |
| [8] | Dodge M., Quinata K, Stinson K. Effective work with Excel/English translation. St. Petersburg, 2000; (in Russian). |
| [9] | Klos A., Wende E., Wareham K. J. et al. International Union of Pharmacology Complement peptide C5a, C4a and C3a reseptors. Pharmacol. Rev. 2013; 65: 500-543. |
| [10] | Kornienko A. A., Kulikovskii N. N., Sorokaty A. E. Catheterization of the thoracic duct in the experiment. Topical issues of topographic anatomy and operative surgery. M., 1977; 1, 22-6 (in Russian). |
| [11] | Reznikova L. S. Complement and its significance in immunological reactions: Moscow; 1967. |
| [12] | McHarg S., Clark S. J., Day A. J. et al. Age-related macular degeneration and the role of the complement system. Molecular Immunology, 2015, 02. 032. |
| [13] | Silva A. S., Teixeria A. G., Bavia L. et al. Plasma levels of complement proteins from the alternative pathway in patients with age-related macular degeneration are independent of Complement Factor H tyr (4) (0) (2) His. polymorphism. Mol. Vis. 2012; 18: 2288-2299. |
APA Style
Aliyeva Tarana Rzakuli. (2019). Determination of Levels of IgE and IgG and the Activity of Complement Comparatively in Blood and Lymph in the Experimental Arthus and Overy Phenomenon. International Journal of Immunology, 7(3), 37-40. https://doi.org/10.11648/j.iji.20190703.12
ACS Style
Aliyeva Tarana Rzakuli. Determination of Levels of IgE and IgG and the Activity of Complement Comparatively in Blood and Lymph in the Experimental Arthus and Overy Phenomenon. Int. J. Immunol. 2019, 7(3), 37-40. doi: 10.11648/j.iji.20190703.12
AMA Style
Aliyeva Tarana Rzakuli. Determination of Levels of IgE and IgG and the Activity of Complement Comparatively in Blood and Lymph in the Experimental Arthus and Overy Phenomenon. Int J Immunol. 2019;7(3):37-40. doi: 10.11648/j.iji.20190703.12
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Download@article{10.11648/j.iji.20190703.12,
author = {Aliyeva Tarana Rzakuli},
title = {Determination of Levels of IgE and IgG and the Activity of Complement Comparatively in Blood and Lymph in the Experimental Arthus and Overy Phenomenon},
journal = {International Journal of Immunology},
volume = {7},
number = {3},
pages = {37-40},
doi = {10.11648/j.iji.20190703.12},
url = {https://doi.org/10.11648/j.iji.20190703.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20190703.12},
abstract = {The aim of investigation was the determination of the levels of IgE, IgG and complement activity in the blood and lymph in the experimental Arthus and Overy phenomenon. Experiments were carried out on 27 chinchilla rabbits (weight 2.5-3kg) in three series. To reproduce the Arthus phenomenon, rabbits were sensitized by subcutaneous administration of 1 ml of horse serum to the scapular region every 5 days; after the fifth injection in the injection area of horse serum the necrosis was observed. To obtain the Overy phenomenon, a resolving dose of horse serum was injected subcutaneously in a sensitized animal, and an Evans blue was introduced into the auricular vein. In this case, the injection area is colored blue. The blood necessary for the experiment was taken from the marginal auricular vein of the rabbit, and lymph – from the thoracic lymphatic duct. The levels of IgE and IgG and the activity of the complement comparatively in the blood and lymph in the experimental Arthus and Overy phenomenon were studied. The experiments were carried out on 27 rabbits of the «Chinchilla» breed in two series. The studied parameters of IgE and IgG and complement activity in blood and lymph of intact animals served as control. The results of the study showed that the level of IgG in the Arthus phenomenon increases and IgE decreases. While at the Overy phenomenon, on the contrary, the level of IgE increases and the level of IgG decreases. The titer of complement in immune complex reactions is sharply reduced; it indicates the involvement of complement with IgG elimination of the allergen.},
year = {2019}
}
TY - JOUR T1 - Determination of Levels of IgE and IgG and the Activity of Complement Comparatively in Blood and Lymph in the Experimental Arthus and Overy Phenomenon AU - Aliyeva Tarana Rzakuli Y1 - 2019/09/27 PY - 2019 N1 - https://doi.org/10.11648/j.iji.20190703.12 DO - 10.11648/j.iji.20190703.12 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 37 EP - 40 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20190703.12 AB - The aim of investigation was the determination of the levels of IgE, IgG and complement activity in the blood and lymph in the experimental Arthus and Overy phenomenon. Experiments were carried out on 27 chinchilla rabbits (weight 2.5-3kg) in three series. To reproduce the Arthus phenomenon, rabbits were sensitized by subcutaneous administration of 1 ml of horse serum to the scapular region every 5 days; after the fifth injection in the injection area of horse serum the necrosis was observed. To obtain the Overy phenomenon, a resolving dose of horse serum was injected subcutaneously in a sensitized animal, and an Evans blue was introduced into the auricular vein. In this case, the injection area is colored blue. The blood necessary for the experiment was taken from the marginal auricular vein of the rabbit, and lymph – from the thoracic lymphatic duct. The levels of IgE and IgG and the activity of the complement comparatively in the blood and lymph in the experimental Arthus and Overy phenomenon were studied. The experiments were carried out on 27 rabbits of the «Chinchilla» breed in two series. The studied parameters of IgE and IgG and complement activity in blood and lymph of intact animals served as control. The results of the study showed that the level of IgG in the Arthus phenomenon increases and IgE decreases. While at the Overy phenomenon, on the contrary, the level of IgE increases and the level of IgG decreases. The titer of complement in immune complex reactions is sharply reduced; it indicates the involvement of complement with IgG elimination of the allergen. VL - 7 IS - 3 ER -
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