Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR

Sudha Bhavanam; Mathew Diggle; Jiaao Yu; Xiaoli Lilly Pang

doi:doi:10.11648/j.ijbse.20210904.11

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Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR

Sudha Bhavanam, Mathew Diggle, Jiaao Yu, Xiaoli Lilly Pang

Published in International Journal of Biomedical Science and Engineering (Volume 9, Issue 4)

Received: 27 August 2021 Accepted: 24 September 2021 Published: 21 October 2021

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Abstract

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an in vitro to test the anti-infectivity activity of the humidifier buffers (A and B, Lumichem^TM). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the Lumichem^TM buffers A and B had a 2 to 3-log₁₀ reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The Lumichem^TM buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.

Published in	International Journal of Biomedical Science and Engineering (Volume 9, Issue 4)
DOI	10.11648/j.ijbse.20210904.11
Page(s)	78-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

SARS-CoV-2, Coronavirus, COVID-19, Virus Inactivation, Buffers, RT-PCR

References

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[4]	Kampf G, Todt D, Pfaender S, Steinmann E: Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents. J. Hosp. Infect. 104 (3), 246-251 (2020).
[5]	Hirose R, Ikegaya H, Naito Y et al.: Reply to Gracely. Clin. Infect. Dis. (2021).
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[7]	Leslie RA, Zhou SS, Macinga DR: Inactivation of SARS-CoV-2 by commercially available alcohol-based hand sanitizers. Am. J. Infect. Control 49 (3), 401-402 (2021).
[8]	Kratzel A, Todt D, V'kovski P et al.: Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols. Emerg. Infect. Dis. 26 (7), 1592-1595 (2020).
[9]	Chen Z, Boon SS, Wang MH, Chan RWY, Chan PKS: Genomic and evolutionary comparison between SARS-CoV-2 and other human coronaviruses. J. Virol. Methods 289, 114032 (2021).
[10]	Ramakrishnan MA: Determination of 50% endpoint titer using a simple formula. World J. Virol. 5 (2), 85-86 (2016).
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[14]	Vijgen L, Keyaerts E, Moes E, Maes P, Duson G, Van Ranst M: Development of one-step, real-time, quantitative reverse transcriptase PCR assays for absolute quantitation of human coronaviruses OC43 and 229E. J. Clin. Microbiol. 43 (11), 5452-5456 (2005).

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APA Style

Sudha Bhavanam, Mathew Diggle, Jiaao Yu, Xiaoli Lilly Pang. (2021). Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR. International Journal of Biomedical Science and Engineering, 9(4), 78-82. https://doi.org/10.11648/j.ijbse.20210904.11

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ACS Style

Sudha Bhavanam; Mathew Diggle; Jiaao Yu; Xiaoli Lilly Pang. Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR. Int. J. Biomed. Sci. Eng. 2021, 9(4), 78-82. doi: 10.11648/j.ijbse.20210904.11

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AMA Style

Sudha Bhavanam, Mathew Diggle, Jiaao Yu, Xiaoli Lilly Pang. Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR. Int J Biomed Sci Eng. 2021;9(4):78-82. doi: 10.11648/j.ijbse.20210904.11

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@article{10.11648/j.ijbse.20210904.11,
  author = {Sudha Bhavanam and Mathew Diggle and Jiaao Yu and Xiaoli Lilly Pang},
  title = {Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {9},
  number = {4},
  pages = {78-82},
  doi = {10.11648/j.ijbse.20210904.11},
  url = {https://doi.org/10.11648/j.ijbse.20210904.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20210904.11},
  abstract = {Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an in vitro to test the anti-infectivity activity of the humidifier buffers (A and B, LumichemTM). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the LumichemTM buffers A and B had a 2 to 3-log10 reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The LumichemTM buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.},
 year = {2021}
}

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TY  - JOUR
T1  - Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR
AU  - Sudha Bhavanam
AU  - Mathew Diggle
AU  - Jiaao Yu
AU  - Xiaoli Lilly Pang
Y1  - 2021/10/21
PY  - 2021
N1  - https://doi.org/10.11648/j.ijbse.20210904.11
DO  - 10.11648/j.ijbse.20210904.11
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 78
EP  - 82
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20210904.11
AB  - Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an in vitro to test the anti-infectivity activity of the humidifier buffers (A and B, LumichemTM). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the LumichemTM buffers A and B had a 2 to 3-log10 reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The LumichemTM buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.
VL  - 9
IS  - 4
ER  -

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Author Information

Sudha Bhavanam

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada
Mathew Diggle

Public Health Laboratory, Alberta Precision Laboratories, Edmonton, Canada
Jiaao Yu

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada
Xiaoli Lilly Pang

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada

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APA Style

Sudha Bhavanam, Mathew Diggle, Jiaao Yu, Xiaoli Lilly Pang. (2021). Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR. International Journal of Biomedical Science and Engineering, 9(4), 78-82. https://doi.org/10.11648/j.ijbse.20210904.11

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ACS Style

Sudha Bhavanam; Mathew Diggle; Jiaao Yu; Xiaoli Lilly Pang. Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR. Int. J. Biomed. Sci. Eng. 2021, 9(4), 78-82. doi: 10.11648/j.ijbse.20210904.11

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AMA Style

Sudha Bhavanam, Mathew Diggle, Jiaao Yu, Xiaoli Lilly Pang. Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR. Int J Biomed Sci Eng. 2021;9(4):78-82. doi: 10.11648/j.ijbse.20210904.11

Copy | Download

@article{10.11648/j.ijbse.20210904.11,
  author = {Sudha Bhavanam and Mathew Diggle and Jiaao Yu and Xiaoli Lilly Pang},
  title = {Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {9},
  number = {4},
  pages = {78-82},
  doi = {10.11648/j.ijbse.20210904.11},
  url = {https://doi.org/10.11648/j.ijbse.20210904.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20210904.11},
  abstract = {Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an in vitro to test the anti-infectivity activity of the humidifier buffers (A and B, LumichemTM). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the LumichemTM buffers A and B had a 2 to 3-log10 reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The LumichemTM buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR
AU  - Sudha Bhavanam
AU  - Mathew Diggle
AU  - Jiaao Yu
AU  - Xiaoli Lilly Pang
Y1  - 2021/10/21
PY  - 2021
N1  - https://doi.org/10.11648/j.ijbse.20210904.11
DO  - 10.11648/j.ijbse.20210904.11
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 78
EP  - 82
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20210904.11
AB  - Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an in vitro to test the anti-infectivity activity of the humidifier buffers (A and B, LumichemTM). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the LumichemTM buffers A and B had a 2 to 3-log10 reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The LumichemTM buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.
VL  - 9
IS  - 4
ER  -

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