Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique

Dawei Wang; Tao Yang; Xiangyi Chen; Feng Guo; Liujun Jia; Guangxin Yue; Ying Kui Liang; Xin Wang

doi:doi:10.11648/j.ijbse.20210903.14

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Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique

Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, Guangxin Yue, Ying Kui Liang, Xin Wang

Published in International Journal of Biomedical Science and Engineering (Volume 9, Issue 3)

Received: 29 July 2021 Accepted: 11 September 2021 Published: 23 September 2021

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Abstract

Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.

Published in	International Journal of Biomedical Science and Engineering (Volume 9, Issue 3)
DOI	10.11648/j.ijbse.20210903.14
Page(s)	73-77
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Atherosclerosis, Rabbits, Balloon Endothelial Injury, Animal Model, CT Imaging

References

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[4]	Cui Wenyu, Du ran. Study on the animal model of carotid atherosclerosis Progress [J]. China urban and rural enterprise health, 2016 (6): 31-32.
[5]	Leber AW, von Ziegler F, Becker A et al (2008) Characteristics of coronary plaques before angiographic progression determined by multi-slice CT. Int J Cardiovasc Imaging 24: 423-428.
[6]	P. Libby, Inflammation in atherosclerosis, Nature 420 (6917) (2002) 868-874.
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[8]	Chen Hua, Xie Zhongchen, Huang Guangyong, et al. Atherosclerosis in Wuzhishan miniature pig Establishment of metamodel. Experimental animal science, 2007, 24 (6): 39-43.
[9]	Bergen W G, Mersanmann H J. Comparative aspect of lipid metabolism: import on contemporary research and use of animal models [J]. Journal of Nutrition. 2005, 135 (11): 2499-2502.
[10]	Forster BA, Weinberg PD. Changes with age in the influence of endogenous nitricoxide on transport ProPerties of therabbit aortic wall near branches. Arterioscler Thromb Vasc Biol. 1997; 17: 1361-1368.
[11]	Haunstetter A, Izumo S. Apoptosis: Basic mechanisms and implicatio ns for cardiovasculardisease. Circ Res. 1998; 82: 1111-1129.
[12]	FAN J, SHIMOYAMADA H, SUN H, et al. Transgenic rabbits expressing human apolipoprotein (a) develop more extensive atherosclerotic lesions in response to a cholesterol-rich diet. Arterioscler Thromb Vascular Biol, 2001, 21: 88-94.
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[14]	Schwartz CJ, Valente AJ, Sprague EA. A modern view of atherosclerosis. Am J Card. 1993; 71 (6): B9-B14.

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APA Style

Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, et al. (2021). Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. International Journal of Biomedical Science and Engineering, 9(3), 73-77. https://doi.org/10.11648/j.ijbse.20210903.14

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ACS Style

Dawei Wang; Tao Yang; Xiangyi Chen; Feng Guo; Liujun Jia, et al. Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. Int. J. Biomed. Sci. Eng. 2021, 9(3), 73-77. doi: 10.11648/j.ijbse.20210903.14

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AMA Style

Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, et al. Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. Int J Biomed Sci Eng. 2021;9(3):73-77. doi: 10.11648/j.ijbse.20210903.14

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@article{10.11648/j.ijbse.20210903.14,
  author = {Dawei Wang and Tao Yang and Xiangyi Chen and Feng Guo and Liujun Jia and Guangxin Yue and Ying Kui Liang and Xin Wang},
  title = {Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {9},
  number = {3},
  pages = {73-77},
  doi = {10.11648/j.ijbse.20210903.14},
  url = {https://doi.org/10.11648/j.ijbse.20210903.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20210903.14},
  abstract = {Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.},
 year = {2021}
}

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TY  - JOUR
T1  - Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique
AU  - Dawei Wang
AU  - Tao Yang
AU  - Xiangyi Chen
AU  - Feng Guo
AU  - Liujun Jia
AU  - Guangxin Yue
AU  - Ying Kui Liang
AU  - Xin Wang
Y1  - 2021/09/23
PY  - 2021
N1  - https://doi.org/10.11648/j.ijbse.20210903.14
DO  - 10.11648/j.ijbse.20210903.14
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 73
EP  - 77
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20210903.14
AB  - Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.
VL  - 9
IS  - 3
ER  -

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Author Information

Dawei Wang

Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, People’s Republic of China
Tao Yang

Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China
Xiangyi Chen

Department of Nuclear Medicine, the First College of Clinical Medical Sciences, China Three Gorges University, Yichang Central People’s Hospital, Yichang, People’s Republic of China
Feng Guo

Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, People’s Republic of China
Liujun Jia

Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China
Guangxin Yue

Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China
Ying Kui Liang

Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, People’s Republic of China
Xin Wang

Department of Cardiac Surgery, Department of Radiology, Fu Wai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China

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APA Style

Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, et al. (2021). Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. International Journal of Biomedical Science and Engineering, 9(3), 73-77. https://doi.org/10.11648/j.ijbse.20210903.14

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ACS Style

Dawei Wang; Tao Yang; Xiangyi Chen; Feng Guo; Liujun Jia, et al. Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. Int. J. Biomed. Sci. Eng. 2021, 9(3), 73-77. doi: 10.11648/j.ijbse.20210903.14

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AMA Style

Dawei Wang, Tao Yang, Xiangyi Chen, Feng Guo, Liujun Jia, et al. Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique. Int J Biomed Sci Eng. 2021;9(3):73-77. doi: 10.11648/j.ijbse.20210903.14

Copy | Download

@article{10.11648/j.ijbse.20210903.14,
  author = {Dawei Wang and Tao Yang and Xiangyi Chen and Feng Guo and Liujun Jia and Guangxin Yue and Ying Kui Liang and Xin Wang},
  title = {Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {9},
  number = {3},
  pages = {73-77},
  doi = {10.11648/j.ijbse.20210903.14},
  url = {https://doi.org/10.11648/j.ijbse.20210903.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20210903.14},
  abstract = {Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique
AU  - Dawei Wang
AU  - Tao Yang
AU  - Xiangyi Chen
AU  - Feng Guo
AU  - Liujun Jia
AU  - Guangxin Yue
AU  - Ying Kui Liang
AU  - Xin Wang
Y1  - 2021/09/23
PY  - 2021
N1  - https://doi.org/10.11648/j.ijbse.20210903.14
DO  - 10.11648/j.ijbse.20210903.14
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 73
EP  - 77
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20210903.14
AB  - Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.
VL  - 9
IS  - 3
ER  -

Copy | Download