The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation

Kayukova Elena; Belokrinitskaya Tatyayna; Sholokhov Leonid; Tereshkov Pavel; Khyshiktuev Bair

doi:doi:10.11648/j.ijbse.20190701.14

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The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation

Kayukova Elena, Belokrinitskaya Tatyayna, Sholokhov Leonid, Tereshkov Pavel, Khyshiktuev Bair

Published in International Journal of Biomedical Science and Engineering (Volume 7, Issue 1)

Received: 5 April 2019 Accepted: 29 May 2019 Published: 18 June 2019

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Abstract

Introduction. There is some data of the short-chain fatty acids (SCFA) participation in cancerogenesis. The cervical cancer is an “ideal” model for determining the molecular mechanisms underlying the development of cancer. The aim of study was to estimate the influence of propionate on the spectrum of SCFA in cervical cells during dis- and neoplastic transformation. Materials and methods of research. Materials of the study - cervical biopsy verified morphologically. Study groups: IA - the focus of the pretumor lesion of cervix; IB - paradisplastic cells; IIA - locus of cervical cancer; IIB - paraneoplastic cells. The spectrum of fatty acids was analyzed before, after 24 hours incubation with 50 μmol/l propionic acid by the gas chromatography method. Methods of nonparametric statistics the Mann-Whitney test were used. Differences were considered statistically significant at p<0.05. Results of the study. The incubation of cervical cancer cells with propionate leads to an increase the level of propionate and butyrate. The incubation of "precancer", paraneoplastic and paradisplastic cells leads to an increase the levels of isobutyric, valeric and caproic acids against the background of a significant drop in the concentration of propionate and butyrate. Сonclusion. It can be concluded that the effect of propionate on the metabolism of fatty acids in the cervical epithelium of the studied samples is multidirectional and depends on the cell type. The dates indicate the modifications of SCFA in the cervical carcinogenesis.

Published in	International Journal of Biomedical Science and Engineering (Volume 7, Issue 1)
DOI	10.11648/j.ijbse.20190701.14
Page(s)	20-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cancerogenesis, Cervical Cancer, Short Chain Fatty Acid

References

[1]	Beskow LM, (2016), Lessons from HeLa Cells: The Ethics and Policy of Biospecimens. Annu Rev Genomics Hum Genet, 17: 395-417.
[2]	Wang G, Yu Y, Wang YZ, Wang JJ, Guan R, Sun Y, Gao F, Shi J, Fu XL, (2019), Role of SCFAs in gut microbiome and glycolysis for colorectal cancer therapy. J Cell Physiol, doi: 10.1002/jcp.28436.
[3]	Zhao L, Bin S, He HL, Jang MY, Pu YC, Gao CH, Wang H, Wang BL, (2018), Sodium butyrate increases P-gp expression in lung cancer by upregulation of STAT3 and mRNA stabilization of ABCB1. Anticancer Drugs, 3: 227-233.
[4]	Li L, Ma L, Fu P, (2017), Gut microbiota-derived short-chain fatty acids and kidney diseases. Drug Des Devel The, 11: 3531-3542.
[5]	Mikó E, Kovács T, Sebő É, Tóth J, Csonka T, Ujlaki G, Sipos A, Szabó J, Méhes G, Bai P, (2019), Microbiome-Microbial Metabolome-Cancer Cell Interactions in Breast Cancer-Familiar, but Unexplored. Cells, 8(4). doi: 10.3390/cells8040293.
[6]	Heers H, Stanislaw J, Harrelson J, Lee MW, (2018), Valproic acid as an adjunctive therapeutic agent for the treatment of breast cancer. Eur J Pharmacol, 835: 61-74.
[7]	Kayukova E, Tereshkov Р, (2015), The short chain fatty acid spectrum of cervical dysplasia and cancer cells. Sovremennye problemy nauki i obrazovaniya, 2-1. URL: http://science-education.ru/ru/article/view?id=18999.
[8]	Kayukova E, Belokrinitskaya Т, Tereshkov Р, (2019), The fatty acid status of the cervical cells during dis-and neoplastic transformation. Fundamental and clinical medicine, 3 (3): 35-44.
[9]	World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects (2013). JAMA, 310: 2191-2194.
[10]	Minushkin O, Ardatskaya MD, (2004), Opportunities and prospects for the study of short-chain fatty acids in the pathology of the gastrointestinal tract on the example of diseases of the intestine and organs of the hepatobiliary system. Clinical laboratory diagnosis, 2: 19-36.
[11]	Kanikarla-Marie P, Kopetz S, Hawk ET, Millward SW, Sood AK, Gresele P, Overman M, Honn K, Menter DG, (2018), Bioactive lipid metabolism in platelet "first responder" and cancer biology. Cancer Metastasis Rev, 37(2-3): 439-454.
[12]	Yi M, Li J, Chen S, Cai J, Ban Y, Peng Q, Zhou Y, Zeng Z, Peng S, Li X, Xiong W, Li G, Xiang B, (2018), Emerging role of lipid metabolism alterations in Cancer stem cells. J Exp Clin Cancer Res, 37(1). doi: 10.1186/s13046-018-0784-5.
[13]	Nakagawa H, Hayata Y, Kawamura S, Yamada T, Fujiwara N, Koike K, (2018,) Lipid Metabolic Reprogramming in Hepatocellular Carcinoma. Cancers (Basel), 10(11). doi: 10.3390/cancers10110447. Review.
[14]	Sivaprakasam S, Prasad PD, Singh N, (2016), Benefits of short-chain fatty acids and their receptors in inflammation and carcinogenesis, 164: 144-51.
[15]	Pope ED, Kimbrough EO, Vemireddy LP, Surapaneni PK, Copland JA 3rd, Mody K, (2019), Aberrant lipid metabolism as a therapeutic target in liver cancer. Expert Opin Ther Targets, 10: 1-11.

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Kayukova Elena, Belokrinitskaya Tatyayna, Sholokhov Leonid, Tereshkov Pavel, Khyshiktuev Bair. (2019). The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation. International Journal of Biomedical Science and Engineering, 7(1), 20-25. https://doi.org/10.11648/j.ijbse.20190701.14

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ACS Style

Kayukova Elena; Belokrinitskaya Tatyayna; Sholokhov Leonid; Tereshkov Pavel; Khyshiktuev Bair. The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation. Int. J. Biomed. Sci. Eng. 2019, 7(1), 20-25. doi: 10.11648/j.ijbse.20190701.14

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AMA Style

Kayukova Elena, Belokrinitskaya Tatyayna, Sholokhov Leonid, Tereshkov Pavel, Khyshiktuev Bair. The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation. Int J Biomed Sci Eng. 2019;7(1):20-25. doi: 10.11648/j.ijbse.20190701.14

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@article{10.11648/j.ijbse.20190701.14,
  author = {Kayukova Elena and Belokrinitskaya Tatyayna and Sholokhov Leonid and Tereshkov Pavel and Khyshiktuev Bair},
  title = {The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {7},
  number = {1},
  pages = {20-25},
  doi = {10.11648/j.ijbse.20190701.14},
  url = {https://doi.org/10.11648/j.ijbse.20190701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20190701.14},
  abstract = {Introduction. There is some data of the short-chain fatty acids (SCFA) participation in cancerogenesis. The cervical cancer is an “ideal” model for determining the molecular mechanisms underlying the development of cancer. The aim of study was to estimate the influence of propionate on the spectrum of SCFA in cervical cells during dis- and neoplastic transformation. Materials and methods of research. Materials of the study - cervical biopsy verified morphologically. Study groups: IA - the focus of the pretumor lesion of cervix; IB - paradisplastic cells; IIA - locus of cervical cancer; IIB - paraneoplastic cells. The spectrum of fatty acids was analyzed before, after 24 hours incubation with 50 μmol/l propionic acid by the gas chromatography method. Methods of nonparametric statistics the Mann-Whitney test were used. Differences were considered statistically significant at p<0.05. Results of the study. The incubation of cervical cancer cells with propionate leads to an increase the level of propionate and butyrate. The incubation of "precancer", paraneoplastic and paradisplastic cells leads to an increase the levels of isobutyric, valeric and caproic acids against the background of a significant drop in the concentration of propionate and butyrate. Сonclusion. It can be concluded that the effect of propionate on the metabolism of fatty acids in the cervical epithelium of the studied samples is multidirectional and depends on the cell type. The dates indicate the modifications of SCFA in the cervical carcinogenesis.},
 year = {2019}
}

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TY - JOUR
T1 - The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation
AU - Kayukova Elena
AU - Belokrinitskaya Tatyayna
AU - Sholokhov Leonid
AU - Tereshkov Pavel
AU - Khyshiktuev Bair
Y1 - 2019/06/18
PY - 2019
N1 - https://doi.org/10.11648/j.ijbse.20190701.14
DO - 10.11648/j.ijbse.20190701.14
T2 - International Journal of Biomedical Science and Engineering
JF - International Journal of Biomedical Science and Engineering
JO - International Journal of Biomedical Science and Engineering
SP - 20
EP - 25
PB - Science Publishing Group
SN - 2376-7235
UR - https://doi.org/10.11648/j.ijbse.20190701.14
AB - Introduction. There is some data of the short-chain fatty acids (SCFA) participation in cancerogenesis. The cervical cancer is an “ideal” model for determining the molecular mechanisms underlying the development of cancer. The aim of study was to estimate the influence of propionate on the spectrum of SCFA in cervical cells during dis- and neoplastic transformation. Materials and methods of research. Materials of the study - cervical biopsy verified morphologically. Study groups: IA - the focus of the pretumor lesion of cervix; IB - paradisplastic cells; IIA - locus of cervical cancer; IIB - paraneoplastic cells. The spectrum of fatty acids was analyzed before, after 24 hours incubation with 50 μmol/l propionic acid by the gas chromatography method. Methods of nonparametric statistics the Mann-Whitney test were used. Differences were considered statistically significant at p<0.05. Results of the study. The incubation of cervical cancer cells with propionate leads to an increase the level of propionate and butyrate. The incubation of "precancer", paraneoplastic and paradisplastic cells leads to an increase the levels of isobutyric, valeric and caproic acids against the background of a significant drop in the concentration of propionate and butyrate. Сonclusion. It can be concluded that the effect of propionate on the metabolism of fatty acids in the cervical epithelium of the studied samples is multidirectional and depends on the cell type. The dates indicate the modifications of SCFA in the cervical carcinogenesis.
VL - 7
IS - 1
ER -

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Author Information

Kayukova Elena

Department of Oncology, Chita state Medical Academy, Chita, Russia
Belokrinitskaya Tatyayna

Department of Obstetrics and Gynecology, Chita State Medical Academy, Chita, Russia
Sholokhov Leonid

Laboratory of Physiology and Pathology of the Endocrine System, Scientific Centre for Family Health and Human Reproduction Problems, Irkutsk, Russia
Tereshkov Pavel

Laboratory of Experimental and Clinical Biochemistry and Immunology, Chita State Medical Academy, Chita, Russia
Khyshiktuev Bair

Management Department, ООО ?Medicom?, Moscow, Russia

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APA Style

Kayukova Elena, Belokrinitskaya Tatyayna, Sholokhov Leonid, Tereshkov Pavel, Khyshiktuev Bair. (2019). The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation. International Journal of Biomedical Science and Engineering, 7(1), 20-25. https://doi.org/10.11648/j.ijbse.20190701.14

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ACS Style

Kayukova Elena; Belokrinitskaya Tatyayna; Sholokhov Leonid; Tereshkov Pavel; Khyshiktuev Bair. The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation. Int. J. Biomed. Sci. Eng. 2019, 7(1), 20-25. doi: 10.11648/j.ijbse.20190701.14

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AMA Style

Kayukova Elena, Belokrinitskaya Tatyayna, Sholokhov Leonid, Tereshkov Pavel, Khyshiktuev Bair. The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation. Int J Biomed Sci Eng. 2019;7(1):20-25. doi: 10.11648/j.ijbse.20190701.14

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@article{10.11648/j.ijbse.20190701.14,
  author = {Kayukova Elena and Belokrinitskaya Tatyayna and Sholokhov Leonid and Tereshkov Pavel and Khyshiktuev Bair},
  title = {The Influence of Propionate on the Spectrum of Short-Chain Fatty Acids in Cervical Cells During Dis- and Neoplastic Transformation},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {7},
  number = {1},
  pages = {20-25},
  doi = {10.11648/j.ijbse.20190701.14},
  url = {https://doi.org/10.11648/j.ijbse.20190701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20190701.14},
  abstract = {Introduction. There is some data of the short-chain fatty acids (SCFA) participation in cancerogenesis. The cervical cancer is an “ideal” model for determining the molecular mechanisms underlying the development of cancer. The aim of study was to estimate the influence of propionate on the spectrum of SCFA in cervical cells during dis- and neoplastic transformation. Materials and methods of research. Materials of the study - cervical biopsy verified morphologically. Study groups: IA - the focus of the pretumor lesion of cervix; IB - paradisplastic cells; IIA - locus of cervical cancer; IIB - paraneoplastic cells. The spectrum of fatty acids was analyzed before, after 24 hours incubation with 50 μmol/l propionic acid by the gas chromatography method. Methods of nonparametric statistics the Mann-Whitney test were used. Differences were considered statistically significant at p<0.05. Results of the study. The incubation of cervical cancer cells with propionate leads to an increase the level of propionate and butyrate. The incubation of "precancer", paraneoplastic and paradisplastic cells leads to an increase the levels of isobutyric, valeric and caproic acids against the background of a significant drop in the concentration of propionate and butyrate. Сonclusion. It can be concluded that the effect of propionate on the metabolism of fatty acids in the cervical epithelium of the studied samples is multidirectional and depends on the cell type. The dates indicate the modifications of SCFA in the cervical carcinogenesis.},
 year = {2019}
}

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