Evaluation of Genome Size, Chromosome Number and Karyotype in <i>Zygophyllum xanthoxylon</i>

Yanbo Wu; Linjing Zhang; Yue Wang; Cui Liu; Shengdan Wu

doi:doi:10.11648/j.eeb.20210602.11

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Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon

Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu

Published in Ecology and Evolutionary Biology (Volume 6, Issue 2)

Received: 31 March 2021 Accepted: 14 April 2021 Published: 23 April 2021

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Abstract

Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.

Published in	Ecology and Evolutionary Biology (Volume 6, Issue 2)
DOI	10.11648/j.eeb.20210602.11
Page(s)	31-37
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Zygophyllum xanthoxylon, Chromosome Number, Karyotype, Flow Cytometry, Genomic Size, Endopolyploidy

References

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APA Style

Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu. (2021). Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecology and Evolutionary Biology, 6(2), 31-37. https://doi.org/10.11648/j.eeb.20210602.11

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ACS Style

Yanbo Wu; Linjing Zhang; Yue Wang; Cui Liu; Shengdan Wu. Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecol. Evol. Biol. 2021, 6(2), 31-37. doi: 10.11648/j.eeb.20210602.11

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AMA Style

Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu. Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecol Evol Biol. 2021;6(2):31-37. doi: 10.11648/j.eeb.20210602.11

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@article{10.11648/j.eeb.20210602.11,
  author = {Yanbo Wu and Linjing Zhang and Yue Wang and Cui Liu and Shengdan Wu},
  title = {Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon},
  journal = {Ecology and Evolutionary Biology},
  volume = {6},
  number = {2},
  pages = {31-37},
  doi = {10.11648/j.eeb.20210602.11},
  url = {https://doi.org/10.11648/j.eeb.20210602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.eeb.20210602.11},
  abstract = {Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.},
 year = {2021}
}

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TY - JOUR
T1 - Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon
AU - Yanbo Wu
AU - Linjing Zhang
AU - Yue Wang
AU - Cui Liu
AU - Shengdan Wu
Y1 - 2021/04/23
PY - 2021
N1 - https://doi.org/10.11648/j.eeb.20210602.11
DO - 10.11648/j.eeb.20210602.11
T2 - Ecology and Evolutionary Biology
JF - Ecology and Evolutionary Biology
JO - Ecology and Evolutionary Biology
SP - 31
EP - 37
PB - Science Publishing Group
SN - 2575-3762
UR - https://doi.org/10.11648/j.eeb.20210602.11
AB - Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.
VL - 6
IS - 2
ER -

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Author Information

Yanbo Wu

College of Life Sciences, Shanxi Normal University, Linfen, China
Linjing Zhang

College of Life Sciences, Shanxi Normal University, Linfen, China
Yue Wang

College of Life Sciences, Shanxi Normal University, Linfen, China
Cui Liu

College of Life Sciences, Shanxi Normal University, Linfen, China
Shengdan Wu

State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology, Lanzhou University, Lanzhou, China

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APA Style

Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu. (2021). Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecology and Evolutionary Biology, 6(2), 31-37. https://doi.org/10.11648/j.eeb.20210602.11

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ACS Style

Yanbo Wu; Linjing Zhang; Yue Wang; Cui Liu; Shengdan Wu. Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecol. Evol. Biol. 2021, 6(2), 31-37. doi: 10.11648/j.eeb.20210602.11

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AMA Style

Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu. Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecol Evol Biol. 2021;6(2):31-37. doi: 10.11648/j.eeb.20210602.11

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@article{10.11648/j.eeb.20210602.11,
  author = {Yanbo Wu and Linjing Zhang and Yue Wang and Cui Liu and Shengdan Wu},
  title = {Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon},
  journal = {Ecology and Evolutionary Biology},
  volume = {6},
  number = {2},
  pages = {31-37},
  doi = {10.11648/j.eeb.20210602.11},
  url = {https://doi.org/10.11648/j.eeb.20210602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.eeb.20210602.11},
  abstract = {Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.},
 year = {2021}
}

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