Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum

Meng Xun; Haifeng Wang; Burong Li; Hongyan He; Qian He; Yonglie Chu

doi:doi:10.11648/j.cmr.20140303.16

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Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum

Meng Xun, Haifeng Wang, Burong Li, Hongyan He, Qian He, Yonglie Chu

Published in Clinical Medicine Research (Volume 3, Issue 3)

Received: 21 May 2014 Accepted: 16 June 2014 Published: 30 June 2014

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Abstract

Objectives: Previous proteome analysis conducted by our group has demonstrated that cytokeratin 8 was overexpressed in HuH-7 cells harboring in vitro transcribed HCV 1b full length RNA (HuH-7-HCV). Present study was aim to verify the results of proteomics, and obtain the clinical data of CK8 expresson levels in HCV patients. Methods: The expression level of CK 8 in HuH-7-HCV cells was examined by Real time PCR and Western blotting. The concentration of CK8 in HCV patients’ serum was measured by enzyme-linked immunosorbent assay (ELISA). Results: The results showed expression level of CK8 transcript in HuH-7-HCV cells was 2.3 fold higher than that in HuH-7 mock cells (P<0.01). The protein expression of CK8 in HuH-7-HCV cells was approximately 3 fold higher than that in HuH-7 mock cells (P<0.01). However, results of ELISA demonstrated the serum CK8 concentration was significantly reduced in chronic HCV patients compared to normal healthy controls (P<0.01). And there was a negative linear correlation between serum CK8 concentration and HCV RNA titer (r=-0.380, P<0.01). Conclusion: Our present study supports the hypothesis that in response to HCV infection, expression of CK8 was increased, which may contribute to the essential cytoprotection provided by CK8 in the liver. Altered CK8 expression pattern could be an important event in the pathogenesis of HCV infection. CK8 have potential use as surrogate markers of liver injury.

Published in	Clinical Medicine Research (Volume 3, Issue 3)
DOI	10.11648/j.cmr.20140303.16
Page(s)	80-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Hepatitis C Virus, Cytokeratin 8, HuH-7-HCV, Serum, Expression Levels

References

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APA Style

Meng Xun, Haifeng Wang, Burong Li, Hongyan He, Qian He, et al. (2014). Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum. Clinical Medicine Research, 3(3), 80-86. https://doi.org/10.11648/j.cmr.20140303.16

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ACS Style

Meng Xun; Haifeng Wang; Burong Li; Hongyan He; Qian He, et al. Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum. Clin. Med. Res. 2014, 3(3), 80-86. doi: 10.11648/j.cmr.20140303.16

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AMA Style

Meng Xun, Haifeng Wang, Burong Li, Hongyan He, Qian He, et al. Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum. Clin Med Res. 2014;3(3):80-86. doi: 10.11648/j.cmr.20140303.16

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@article{10.11648/j.cmr.20140303.16,
  author = {Meng Xun and Haifeng Wang and Burong Li and Hongyan He and Qian He and Yonglie Chu},
  title = {Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum},
  journal = {Clinical Medicine Research},
  volume = {3},
  number = {3},
  pages = {80-86},
  doi = {10.11648/j.cmr.20140303.16},
  url = {https://doi.org/10.11648/j.cmr.20140303.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20140303.16},
  abstract = {Objectives: Previous proteome analysis conducted by our group has demonstrated that cytokeratin 8 was overexpressed in HuH-7 cells harboring in vitro transcribed HCV 1b full length RNA (HuH-7-HCV). Present study was aim to verify the results of proteomics, and obtain the clinical data of CK8 expresson levels in HCV patients. Methods: The expression level of CK 8 in HuH-7-HCV cells was examined by Real time PCR and Western blotting. The concentration of CK8 in HCV patients’ serum was measured by enzyme-linked immunosorbent assay (ELISA). Results: The results showed expression level of CK8 transcript in HuH-7-HCV cells was 2.3 fold higher than that in HuH-7 mock cells (P<0.01). The protein expression of CK8 in HuH-7-HCV cells was approximately 3 fold higher than that in HuH-7 mock cells (P<0.01). However, results of ELISA demonstrated the serum CK8 concentration was significantly reduced in chronic HCV patients compared to normal healthy controls (P<0.01). And there was a negative linear correlation between serum CK8 concentration and HCV RNA titer (r=-0.380, P<0.01). Conclusion: Our present study supports the hypothesis that in response to HCV infection, expression of CK8 was increased, which may contribute to the essential cytoprotection provided by CK8 in the liver. Altered CK8 expression pattern could be an important event in the pathogenesis of HCV infection. CK8 have potential use as surrogate markers of liver injury.},
 year = {2014}
}

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TY - JOUR
T1 - Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum
AU - Meng Xun
AU - Haifeng Wang
AU - Burong Li
AU - Hongyan He
AU - Qian He
AU - Yonglie Chu
Y1 - 2014/06/30
PY - 2014
N1 - https://doi.org/10.11648/j.cmr.20140303.16
DO - 10.11648/j.cmr.20140303.16
T2 - Clinical Medicine Research
JF - Clinical Medicine Research
JO - Clinical Medicine Research
SP - 80
EP - 86
PB - Science Publishing Group
SN - 2326-9057
UR - https://doi.org/10.11648/j.cmr.20140303.16
AB - Objectives: Previous proteome analysis conducted by our group has demonstrated that cytokeratin 8 was overexpressed in HuH-7 cells harboring in vitro transcribed HCV 1b full length RNA (HuH-7-HCV). Present study was aim to verify the results of proteomics, and obtain the clinical data of CK8 expresson levels in HCV patients. Methods: The expression level of CK 8 in HuH-7-HCV cells was examined by Real time PCR and Western blotting. The concentration of CK8 in HCV patients’ serum was measured by enzyme-linked immunosorbent assay (ELISA). Results: The results showed expression level of CK8 transcript in HuH-7-HCV cells was 2.3 fold higher than that in HuH-7 mock cells (P<0.01). The protein expression of CK8 in HuH-7-HCV cells was approximately 3 fold higher than that in HuH-7 mock cells (P<0.01). However, results of ELISA demonstrated the serum CK8 concentration was significantly reduced in chronic HCV patients compared to normal healthy controls (P<0.01). And there was a negative linear correlation between serum CK8 concentration and HCV RNA titer (r=-0.380, P<0.01). Conclusion: Our present study supports the hypothesis that in response to HCV infection, expression of CK8 was increased, which may contribute to the essential cytoprotection provided by CK8 in the liver. Altered CK8 expression pattern could be an important event in the pathogenesis of HCV infection. CK8 have potential use as surrogate markers of liver injury.
VL - 3
IS - 3
ER -

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Author Information

Meng Xun

Department of Immunology and Microbiology, Medical School of Xi’an Jiaotong University, Xi’an, Shannxi, China
Haifeng Wang

Department of Clinical Laboratories, Xi’an Central Hospital, Xi’an, Shannxi, China
Burong Li

Department of Clinical Laboratories, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University, Xi’an, Shannxi, China
Hongyan He

Department of Medical Technology, Xi’an Medical College, Xi’an, Shannxi, China
Qian He

Department of Clinical Laboratories, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University, Xi’an, Shannxi, China
Yonglie Chu

Department of Immunology and Microbiology, Medical School of Xi’an Jiaotong University, Xi’an, Shannxi, China

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APA Style

Meng Xun, Haifeng Wang, Burong Li, Hongyan He, Qian He, et al. (2014). Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum. Clinical Medicine Research, 3(3), 80-86. https://doi.org/10.11648/j.cmr.20140303.16

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Meng Xun; Haifeng Wang; Burong Li; Hongyan He; Qian He, et al. Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum. Clin. Med. Res. 2014, 3(3), 80-86. doi: 10.11648/j.cmr.20140303.16

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AMA Style

Meng Xun, Haifeng Wang, Burong Li, Hongyan He, Qian He, et al. Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum. Clin Med Res. 2014;3(3):80-86. doi: 10.11648/j.cmr.20140303.16

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@article{10.11648/j.cmr.20140303.16,
  author = {Meng Xun and Haifeng Wang and Burong Li and Hongyan He and Qian He and Yonglie Chu},
  title = {Cytokeratin 8 Was Over-Expressed in Cells Harboring in Vitro-Transcribed Full Length Hepatitis C Virus 1b RNA, but Down-Expressed in HCV Patients’ Serum},
  journal = {Clinical Medicine Research},
  volume = {3},
  number = {3},
  pages = {80-86},
  doi = {10.11648/j.cmr.20140303.16},
  url = {https://doi.org/10.11648/j.cmr.20140303.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20140303.16},
  abstract = {Objectives: Previous proteome analysis conducted by our group has demonstrated that cytokeratin 8 was overexpressed in HuH-7 cells harboring in vitro transcribed HCV 1b full length RNA (HuH-7-HCV). Present study was aim to verify the results of proteomics, and obtain the clinical data of CK8 expresson levels in HCV patients. Methods: The expression level of CK 8 in HuH-7-HCV cells was examined by Real time PCR and Western blotting. The concentration of CK8 in HCV patients’ serum was measured by enzyme-linked immunosorbent assay (ELISA). Results: The results showed expression level of CK8 transcript in HuH-7-HCV cells was 2.3 fold higher than that in HuH-7 mock cells (P<0.01). The protein expression of CK8 in HuH-7-HCV cells was approximately 3 fold higher than that in HuH-7 mock cells (P<0.01). However, results of ELISA demonstrated the serum CK8 concentration was significantly reduced in chronic HCV patients compared to normal healthy controls (P<0.01). And there was a negative linear correlation between serum CK8 concentration and HCV RNA titer (r=-0.380, P<0.01). Conclusion: Our present study supports the hypothesis that in response to HCV infection, expression of CK8 was increased, which may contribute to the essential cytoprotection provided by CK8 in the liver. Altered CK8 expression pattern could be an important event in the pathogenesis of HCV infection. CK8 have potential use as surrogate markers of liver injury.},
 year = {2014}
}

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