Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks

Sunanda Sharma; Narendra K. Sharma; N. K. Sinha; S. S. Sharma

doi:doi:10.11648/j.avs.20130102.12

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Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks

Sunanda Sharma, Narendra K. Sharma, N. K. Sinha, S. S. Sharma

Published in Animal and Veterinary Sciences (Volume 1, Issue 2)

Published: 10 March 2013

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Abstract

Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.

Published in	Animal and Veterinary Sciences (Volume 1, Issue 2)
DOI	10.11648/j.avs.20130102.12
Page(s)	18-22
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Sirohi Buck, Frozen semen, Seminal Plasma, Transferases, GOT, GPT

References

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APA Style

Sunanda Sharma, Narendra K. Sharma, N. K. Sinha, S. S. Sharma. (2013). Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks. Animal and Veterinary Sciences, 1(2), 18-22. https://doi.org/10.11648/j.avs.20130102.12

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ACS Style

Sunanda Sharma; Narendra K. Sharma; N. K. Sinha; S. S. Sharma. Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks. Anim. Vet. Sci. 2013, 1(2), 18-22. doi: 10.11648/j.avs.20130102.12

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AMA Style

Sunanda Sharma, Narendra K. Sharma, N. K. Sinha, S. S. Sharma. Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks. Anim Vet Sci. 2013;1(2):18-22. doi: 10.11648/j.avs.20130102.12

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@article{10.11648/j.avs.20130102.12,
  author = {Sunanda Sharma and Narendra K. Sharma and N. K. Sinha and S. S. Sharma},
  title = {Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks},
  journal = {Animal and Veterinary Sciences},
  volume = {1},
  number = {2},
  pages = {18-22},
  doi = {10.11648/j.avs.20130102.12},
  url = {https://doi.org/10.11648/j.avs.20130102.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20130102.12},
  abstract = {Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.},
 year = {2013}
}

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TY  - JOUR
T1  - Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks
AU  - Sunanda Sharma
AU  - Narendra K. Sharma
AU  - N. K. Sinha
AU  - S. S. Sharma
Y1  - 2013/03/10
PY  - 2013
N1  - https://doi.org/10.11648/j.avs.20130102.12
DO  - 10.11648/j.avs.20130102.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 18
EP  - 22
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20130102.12
AB  - Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.
VL  - 1
IS  - 2
ER  -

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Author Information

Sunanda Sharma

Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Rajasthan University of Veterinary & Animal Sciences, Bikaner-334001, Rajasthan, India
Narendra K. Sharma

Divisional Mobile Veterinary Surgery and Infertility Unit, Animal husbandry Department, Government of Rajasthan, Bikaner, India
N. K. Sinha

Former Principal Scientist (Animal Reproduction), Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh, India
S. S. Sharma

Former Professor and Head, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Rajasthan University of Veterinary & Animal Sciences, Bikaner-334001, Rajasthan, India

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Sunanda Sharma, Narendra K. Sharma, N. K. Sinha, S. S. Sharma. (2013). Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks. Animal and Veterinary Sciences, 1(2), 18-22. https://doi.org/10.11648/j.avs.20130102.12

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ACS Style

Sunanda Sharma; Narendra K. Sharma; N. K. Sinha; S. S. Sharma. Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks. Anim. Vet. Sci. 2013, 1(2), 18-22. doi: 10.11648/j.avs.20130102.12

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AMA Style

Sunanda Sharma, Narendra K. Sharma, N. K. Sinha, S. S. Sharma. Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks. Anim Vet Sci. 2013;1(2):18-22. doi: 10.11648/j.avs.20130102.12

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@article{10.11648/j.avs.20130102.12,
  author = {Sunanda Sharma and Narendra K. Sharma and N. K. Sinha and S. S. Sharma},
  title = {Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks},
  journal = {Animal and Veterinary Sciences},
  volume = {1},
  number = {2},
  pages = {18-22},
  doi = {10.11648/j.avs.20130102.12},
  url = {https://doi.org/10.11648/j.avs.20130102.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20130102.12},
  abstract = {Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.},
 year = {2013}
}

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TY  - JOUR
T1  - Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks
AU  - Sunanda Sharma
AU  - Narendra K. Sharma
AU  - N. K. Sinha
AU  - S. S. Sharma
Y1  - 2013/03/10
PY  - 2013
N1  - https://doi.org/10.11648/j.avs.20130102.12
DO  - 10.11648/j.avs.20130102.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 18
EP  - 22
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20130102.12
AB  - Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.
VL  - 1
IS  - 2
ER  -

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