The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers

Oluwalana Olumayowa Adedunke; Odebode Adegboyega Christopher; Ayodele Adegboyega Sobowale; Adewuyi-Samuel Oluwatoke Bolatito

doi:doi:10.11648/j.ajpb.20220701.21

The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers

Oluwalana Olumayowa Adedunke, Odebode Adegboyega Christopher, Ayodele Adegboyega Sobowale, Adewuyi-Samuel Oluwatoke Bolatito

Published in American Journal of Plant Biology (Volume 7, Issue 1)

Received: 14 February 2022 Accepted: 2 March 2022 Published: 23 March 2022

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Abstract

Fresh cassava (Manihot esculenta) tubers unlike most other tubers of root crops are highly perishable. This study, therefore, investigated the antifungal activities of ethanol and hot water extract of Melanthera scandens (vine) and Mimosa pudica (touch me not) on rot fungi of cassava. Rotten cassava tuber samples were inoculated into Potato Dextrose Agar (PDA) for isolation of rot fungi causing postharvest deterioration of cassava tubers. The fungi obtained were identified using macroscopic and microscopic characteristics. The establishment of the pathogenicity of the fungal isolates was carried out by inoculating freshly harvested cassava tubers with the isolated fungi. The ethanol and hot water extract of the selected plants were tested against the isolated fungi both in vitro and in vivo. The isolates were identified as Aspergillus niger, Aspergillus flavus, Penicillium notatum, Penicillium sp, and Fusarium verticillioides. A. flavus being the most virulent with a percentage in severity of (27.15) on day 7 as compared with Aspergillus niger, Fusarium verticillioides, Penicillium sp, and Penicillium notatum which had (25.22, 17.17, 15.76 and 14.71) respectively. In the in vitro test the plants exhibited a high degree of fungicidal activity by inhibiting the mycelia growth of the rot fungi and Mimosa pudica was more effective in the suppression of fungal growth than Melanthera scandens. In the in vivo test there was a higher percentage reduction in diameter of rot when the plant extract was applied after twenty four hours of microbial inoculation directly to the cassava tuber. The extracts of Melanthera scandens and Mimosa pudica can therefore be used as a bio fungicide to extend the shelf-life of freshly harvested cassava tubers.

Published in	American Journal of Plant Biology (Volume 7, Issue 1)
DOI	10.11648/j.ajpb.20220701.21
Page(s)	73-80
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Cassava, Fungicidal, Rot - Fungi, Postharvest Deterioration

References

[1]	FAO (Food and Agriculture Organization of the United Nations) Yearbook, (2007). Website: www. fao. org.
[2]	Ziska, L., Runion, G. B., Tomecek, M., Prior, S. A., Torbet, H. A. and Sicher, R. 2009. An evaluation of cassava, sweet potato and field corn as potential carbohydrate sources for bioethanol production in Alabama and Maryland. Biomass and Bioenergy 33: 1503-1508.
[3]	Nweke, F. I., Dunstan, S. C. and John, K. L. 2002. The cassava transformation: Africa best kept secret. Lansing, Mich., USA: Michigan State University Press.
[4]	Tewe, O. O. and Lutaladio, N. 2004. Cassava for livestock feed in subSaharan Africa. Rome, Italy: FAO.
[5]	FAO (2013)-Food and Agriculture Organization of the United Nations. Statistical Database_FAOSTAT from http://faostat.fao.org.
[6]	Saranraj P., Sudhanshu S. B. and Ramesh C. R 2019. Traditional foods from tropical roots and tuber crops: Innovation challenges. Innovations in traditional foods.
[7]	Onyewoke, A., Simoyan, K. J. 2014. Cassava postharvest harvesting processing and storagein Nigeria: A review. African Joournal of Agricultural Research 9. 53: 3853-3863.
[8]	Andrew, W. 2002. Cassava utilization, storage, and small scale processing. Natural resource institute, Chatham maritime. UK. 14: 270-290.
[9]	Sánchez, H., Ceballos, F., Calle, J. C., Pérez, C., Egesi et. al. 2010. Tolerance to Postharvest Physiological Deterioration in Cassava Roots. Crop Sci 50. 4: 1333-1338.
[10]	Booth, R. H., De Bulche, T. S., Cardenas, O. S., Gomez, G. and Hervas, E. 1976. Changes in quality of cassava roots during storage. J. Food Technol 11: 245-264.
[11]	Buschmann, H., Rodriguez, M. X., Tohme, J., Beeching, J. R., 2000. Accumulation of hydroxycoumarin during post-harvest deterioration of tuberous roots of cassava (Manihot esculenta Crantz). Ann. Bot. 86: 1153-1160.
[12]	Westby, A. 2002. Cassava utilization, storage and small-scale processing. In: Cassava: Biology, Production and Utilization. RJ Hillocks, JM Thresh, AC Belloti, Eds, CAB international Publishing, Wallingford, UK, 281-300.
[13]	Lyer S., Mattinson D. S., Fellman, J. K. 2010. Study of the early events leading to cassava root postharvest deterioration. Tropical Plant Biology. 3: 151–165.
[14]	Arya, A. 2010. Recent advnces in the management of plant pathogens: Botanicals in the fungal pest management. In: Management of fungal plant pathogens. Eds. A. Arya &A. E. Perello. UK. CAB international. 1-25.
[15]	Ukeh, J. A. and Chiejina, N. V. 2012. Preliminary investigation of the cause of postharvest fungal rot of tomato. Journal of Pharmacy and Biological sciences 4. 5: 36-39.
[16]	Shukla, A. M., Yadav, R. S., Shashi, S. K. and Dikshit, A. 2012. Use of plant metabolites as an effective source for the management of postharvest fungal pest: A review. Int J Curr Discoveries Innovations 1. 1: 33-45.
[17]	Tripathi, P. and Dubey, A. K. 2004. Exploitation of natural plant products as analternative strategy for control of postharvest fungal rotting of fruits and vegetables. Postharvest Biol Tech 32. 3: 235-245.
[18]	Amaadioha, A. C. and Markson, A. A, 2007. Control of storage rot of cassava tuber caused by Rhizopus Oryzae using some plant extracts. Arch Phytopathol 40. 6: 381-388.
[19]	Odebode, A. C., Jonker, S. A., Joseph, C. C. and Wachira, S. W. 2006. Anti-fungal activities of constituents from Uvaria scheffleri and Abotrys brachpet alus. Journal ofAgricultural sciences51. 1: 79-86.
[20]	Ubuala, A. O. and Oti, E. 2008. Evaluation of Antimicrobial Properties of Some Medicinal Plants for Fresh Cassava Roots Preservation. Pakistan Journal of Nutrition 7. 5: 679-68120. Ubuala, A. O. and Oti, E. 2008. Evaluation of Antimicrobial Properties of Some Medicinal Plants for Fresh Cassava Roots Preservation. Pakistan Journal of Nutrition 7. 5: 679-681.
[21]	Tijjani, A; Adebitan, S. A.; Gurama; A. U; Aliyu, M; Dawa kiji, A. Y. et. al. 2013). Efficacy of Some Botanicals for the Control of Wet Rot Disease on Mechanically Injured Sweet Potato Caused by Rhizopus Stolonifer in Bauchi State. International Journal of Scientific and Research Publications, 3. 6: 2250-3153.
[22]	Odebode, A. C. and Che, A. N. 2001. Control of fungal rot of Citrus sinensis with some medicinal plant extracts in South- Western in Nigeria. Arch. Phythopath 34: 223-233.
[23]	Okigbo, R. N., Putheti, R. and Achusi C. T. 2009a. Post-harvest deterioration of cassava and its control using extracts of Azardirachta indica and Aframonium melegueta. E-JChem 64: 1274-1280.
[24]	Onuegbu, B. A. 2002. Fundamentals of Crop Protection. Agro-science Consult and Extension Unit. RSUT. 237.
[25]	Frank, C. O. and Kingsley, C. A. 2014. Role of Fungal Rots in Post-harvest Storage Loses in Some Nigerian Varieties of Dioscorea Species. Br. Microbiol Res J. 4: 343-350.
[26]	Aidoo, K. A. 2007. Identification of yam tuber rot fungi from storage systems at the Kumasi Central market. Imo state University, Nigeria.
[27]	Kaur, P., Nilesh K., Shivananda T. N. andGagandeep K. 2011. Journal of Medicinal Plants Research 5. 22: 5356-5359.
[28]	Okigbo, R., Nmeka, I. 2005. Control of yam tuber rot with leaf extracts of Xylopia aethiopica and Zingiber officinale. African Journal of Biotechnology4. 8: 804-807.
[29]	Tamilarasi, T. and Ananthi, T. 2012. Phytochemical analysis and antimicrobial activity of Mimosa pudica Linn. Res J Chem Sci 2: 72-74.
[30]	Fagbohun, E. D., Lawal, O. U. and Ore M. E. 2012. The antifungal activity of methanolic crude extract of the leaves of Ocimum gratissimum L., Melanthera scandens A and Leea guineensis L. on some pathogenic fungi. International journal of biology, Pharmacy and Allied Sciences1. 1: 12-21.

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Oluwalana Olumayowa Adedunke, Odebode Adegboyega Christopher, Ayodele Adegboyega Sobowale, Adewuyi-Samuel Oluwatoke Bolatito. (2022). The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers. American Journal of Plant Biology, 7(1), 73-80. https://doi.org/10.11648/j.ajpb.20220701.21

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ACS Style

Oluwalana Olumayowa Adedunke; Odebode Adegboyega Christopher; Ayodele Adegboyega Sobowale; Adewuyi-Samuel Oluwatoke Bolatito. The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers. Am. J. Plant Biol. 2022, 7(1), 73-80. doi: 10.11648/j.ajpb.20220701.21

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AMA Style

Oluwalana Olumayowa Adedunke, Odebode Adegboyega Christopher, Ayodele Adegboyega Sobowale, Adewuyi-Samuel Oluwatoke Bolatito. The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers. Am J Plant Biol. 2022;7(1):73-80. doi: 10.11648/j.ajpb.20220701.21

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@article{10.11648/j.ajpb.20220701.21,
  author = {Oluwalana Olumayowa Adedunke and Odebode Adegboyega Christopher and Ayodele Adegboyega Sobowale and Adewuyi-Samuel Oluwatoke Bolatito},
  title = {The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers},
  journal = {American Journal of Plant Biology},
  volume = {7},
  number = {1},
  pages = {73-80},
  doi = {10.11648/j.ajpb.20220701.21},
  url = {https://doi.org/10.11648/j.ajpb.20220701.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20220701.21},
  abstract = {Fresh cassava (Manihot esculenta) tubers unlike most other tubers of root crops are highly perishable. This study, therefore, investigated the antifungal activities of ethanol and hot water extract of Melanthera scandens (vine) and Mimosa pudica (touch me not) on rot fungi of cassava. Rotten cassava tuber samples were inoculated into Potato Dextrose Agar (PDA) for isolation of rot fungi causing postharvest deterioration of cassava tubers. The fungi obtained were identified using macroscopic and microscopic characteristics. The establishment of the pathogenicity of the fungal isolates was carried out by inoculating freshly harvested cassava tubers with the isolated fungi. The ethanol and hot water extract of the selected plants were tested against the isolated fungi both in vitro and in vivo. The isolates were identified as Aspergillus niger, Aspergillus flavus, Penicillium notatum, Penicillium sp, and Fusarium verticillioides. A. flavus being the most virulent with a percentage in severity of (27.15) on day 7 as compared with Aspergillus niger, Fusarium verticillioides, Penicillium sp, and Penicillium notatum which had (25.22, 17.17, 15.76 and 14.71) respectively. In the in vitro test the plants exhibited a high degree of fungicidal activity by inhibiting the mycelia growth of the rot fungi and Mimosa pudica was more effective in the suppression of fungal growth than Melanthera scandens. In the in vivo test there was a higher percentage reduction in diameter of rot when the plant extract was applied after twenty four hours of microbial inoculation directly to the cassava tuber. The extracts of Melanthera scandens and Mimosa pudica can therefore be used as a bio fungicide to extend the shelf-life of freshly harvested cassava tubers.},
 year = {2022}
}

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TY  - JOUR
T1  - The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers
AU  - Oluwalana Olumayowa Adedunke
AU  - Odebode Adegboyega Christopher
AU  - Ayodele Adegboyega Sobowale
AU  - Adewuyi-Samuel Oluwatoke Bolatito
Y1  - 2022/03/23
PY  - 2022
N1  - https://doi.org/10.11648/j.ajpb.20220701.21
DO  - 10.11648/j.ajpb.20220701.21
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 73
EP  - 80
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20220701.21
AB  - Fresh cassava (Manihot esculenta) tubers unlike most other tubers of root crops are highly perishable. This study, therefore, investigated the antifungal activities of ethanol and hot water extract of Melanthera scandens (vine) and Mimosa pudica (touch me not) on rot fungi of cassava. Rotten cassava tuber samples were inoculated into Potato Dextrose Agar (PDA) for isolation of rot fungi causing postharvest deterioration of cassava tubers. The fungi obtained were identified using macroscopic and microscopic characteristics. The establishment of the pathogenicity of the fungal isolates was carried out by inoculating freshly harvested cassava tubers with the isolated fungi. The ethanol and hot water extract of the selected plants were tested against the isolated fungi both in vitro and in vivo. The isolates were identified as Aspergillus niger, Aspergillus flavus, Penicillium notatum, Penicillium sp, and Fusarium verticillioides. A. flavus being the most virulent with a percentage in severity of (27.15) on day 7 as compared with Aspergillus niger, Fusarium verticillioides, Penicillium sp, and Penicillium notatum which had (25.22, 17.17, 15.76 and 14.71) respectively. In the in vitro test the plants exhibited a high degree of fungicidal activity by inhibiting the mycelia growth of the rot fungi and Mimosa pudica was more effective in the suppression of fungal growth than Melanthera scandens. In the in vivo test there was a higher percentage reduction in diameter of rot when the plant extract was applied after twenty four hours of microbial inoculation directly to the cassava tuber. The extracts of Melanthera scandens and Mimosa pudica can therefore be used as a bio fungicide to extend the shelf-life of freshly harvested cassava tubers.
VL  - 7
IS  - 1
ER  -

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Author Information

Oluwalana Olumayowa Adedunke

Department of Botany, University of Ibadan, Ibadan, Nigeria
Odebode Adegboyega Christopher

Department of Botany, University of Ibadan, Ibadan, Nigeria
Ayodele Adegboyega Sobowale

Department of Botany, University of Ibadan, Ibadan, Nigeria
Adewuyi-Samuel Oluwatoke Bolatito

Biology Department, The Polythechnic, Ibadan, Nigeria

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APA Style

Oluwalana Olumayowa Adedunke, Odebode Adegboyega Christopher, Ayodele Adegboyega Sobowale, Adewuyi-Samuel Oluwatoke Bolatito. (2022). The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers. American Journal of Plant Biology, 7(1), 73-80. https://doi.org/10.11648/j.ajpb.20220701.21

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ACS Style

Oluwalana Olumayowa Adedunke; Odebode Adegboyega Christopher; Ayodele Adegboyega Sobowale; Adewuyi-Samuel Oluwatoke Bolatito. The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers. Am. J. Plant Biol. 2022, 7(1), 73-80. doi: 10.11648/j.ajpb.20220701.21

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AMA Style

Oluwalana Olumayowa Adedunke, Odebode Adegboyega Christopher, Ayodele Adegboyega Sobowale, Adewuyi-Samuel Oluwatoke Bolatito. The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers. Am J Plant Biol. 2022;7(1):73-80. doi: 10.11648/j.ajpb.20220701.21

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@article{10.11648/j.ajpb.20220701.21,
  author = {Oluwalana Olumayowa Adedunke and Odebode Adegboyega Christopher and Ayodele Adegboyega Sobowale and Adewuyi-Samuel Oluwatoke Bolatito},
  title = {The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers},
  journal = {American Journal of Plant Biology},
  volume = {7},
  number = {1},
  pages = {73-80},
  doi = {10.11648/j.ajpb.20220701.21},
  url = {https://doi.org/10.11648/j.ajpb.20220701.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20220701.21},
  abstract = {Fresh cassava (Manihot esculenta) tubers unlike most other tubers of root crops are highly perishable. This study, therefore, investigated the antifungal activities of ethanol and hot water extract of Melanthera scandens (vine) and Mimosa pudica (touch me not) on rot fungi of cassava. Rotten cassava tuber samples were inoculated into Potato Dextrose Agar (PDA) for isolation of rot fungi causing postharvest deterioration of cassava tubers. The fungi obtained were identified using macroscopic and microscopic characteristics. The establishment of the pathogenicity of the fungal isolates was carried out by inoculating freshly harvested cassava tubers with the isolated fungi. The ethanol and hot water extract of the selected plants were tested against the isolated fungi both in vitro and in vivo. The isolates were identified as Aspergillus niger, Aspergillus flavus, Penicillium notatum, Penicillium sp, and Fusarium verticillioides. A. flavus being the most virulent with a percentage in severity of (27.15) on day 7 as compared with Aspergillus niger, Fusarium verticillioides, Penicillium sp, and Penicillium notatum which had (25.22, 17.17, 15.76 and 14.71) respectively. In the in vitro test the plants exhibited a high degree of fungicidal activity by inhibiting the mycelia growth of the rot fungi and Mimosa pudica was more effective in the suppression of fungal growth than Melanthera scandens. In the in vivo test there was a higher percentage reduction in diameter of rot when the plant extract was applied after twenty four hours of microbial inoculation directly to the cassava tuber. The extracts of Melanthera scandens and Mimosa pudica can therefore be used as a bio fungicide to extend the shelf-life of freshly harvested cassava tubers.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - The Effect of Melanthera Scandens and Mimosa Pudica on Fungi Causing Postharvest Deterioration of Cassava Root Tubers
AU  - Oluwalana Olumayowa Adedunke
AU  - Odebode Adegboyega Christopher
AU  - Ayodele Adegboyega Sobowale
AU  - Adewuyi-Samuel Oluwatoke Bolatito
Y1  - 2022/03/23
PY  - 2022
N1  - https://doi.org/10.11648/j.ajpb.20220701.21
DO  - 10.11648/j.ajpb.20220701.21
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 73
EP  - 80
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20220701.21
AB  - Fresh cassava (Manihot esculenta) tubers unlike most other tubers of root crops are highly perishable. This study, therefore, investigated the antifungal activities of ethanol and hot water extract of Melanthera scandens (vine) and Mimosa pudica (touch me not) on rot fungi of cassava. Rotten cassava tuber samples were inoculated into Potato Dextrose Agar (PDA) for isolation of rot fungi causing postharvest deterioration of cassava tubers. The fungi obtained were identified using macroscopic and microscopic characteristics. The establishment of the pathogenicity of the fungal isolates was carried out by inoculating freshly harvested cassava tubers with the isolated fungi. The ethanol and hot water extract of the selected plants were tested against the isolated fungi both in vitro and in vivo. The isolates were identified as Aspergillus niger, Aspergillus flavus, Penicillium notatum, Penicillium sp, and Fusarium verticillioides. A. flavus being the most virulent with a percentage in severity of (27.15) on day 7 as compared with Aspergillus niger, Fusarium verticillioides, Penicillium sp, and Penicillium notatum which had (25.22, 17.17, 15.76 and 14.71) respectively. In the in vitro test the plants exhibited a high degree of fungicidal activity by inhibiting the mycelia growth of the rot fungi and Mimosa pudica was more effective in the suppression of fungal growth than Melanthera scandens. In the in vivo test there was a higher percentage reduction in diameter of rot when the plant extract was applied after twenty four hours of microbial inoculation directly to the cassava tuber. The extracts of Melanthera scandens and Mimosa pudica can therefore be used as a bio fungicide to extend the shelf-life of freshly harvested cassava tubers.
VL  - 7
IS  - 1
ER  -

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