Ficus sycomorus Plant was known traditionally for its medicinal properties, Shigella species as a bacterial was also known for their resistance to orthodox medicine. Hence the synthesis of silver nanoparticles from Ficus sycomorus. This study was carried out to investigate the anti-shigellosis potential of silver nanoparticles synthesized from Ficus sycomorus Linn stem bark aqueous extract against Multi-drug Resistant (MDR) Shigella species isolated from clinical specimen collected from patients attending Yobe State Specialist Hospital Damaturu, Nigeria. A total of 400 diarrhoeagenic stools were screened for isolation of Shigella species and determined their antibiotic susceptibility pattern using standard methods. Phytochemical constituents of Ficus sycomorus extract were used to synthesize silver nanoparticles using green synthesis approach. The nanoparticles was analyzed for transmittance, functional groups, sizes and shapes using Uv-vis, FTIR and Scanning Electron Microscopy (SEM), and was tested for antibacterial activities on MDR Shigella isolates. There is no significant difference in Shigella recovery relation to patients gender (P<0.05). The age group, 0 - 10 years were more susceptible, 40% (36), followed by >30 years (21). Shigella were also found to be sensitive to Ciprofloxacin (92%), Augmentin (87%), Cefuroxime (85%), Streptomycin (83.5%) while the most frequent resistance was showcased against Nalidixic Acid (48%), and Tetracycline (27%). Phytochemicals detected include saponins, flavonoids, alkaloids, cardiac glycoside and tannin. Uv-vis showed broad peaks around 460nm, the FTIR showed C-H stretch of hydroxyl group of alkanes and the SEM showed nanoparticles with wide range of shapes and sizes. Anti-Shigella activities of silver nanoparticles is higher at zones of inhibition between 10mm and 30mm higher compared to the activities of crude aqueous extract and AgNO3 solution against the MDR Shigella species which showed an enhanced activities. The high prevalence of shigellosis among children in this study, indicated that improved hygiene is needed for children in the area and detailed examination is required for the treatment of diarrhoea in adults. Ciprofloxacin and Amoxicillin Clavulanate, Nalidixic acid could be used only where culture and sensitivity results prevailed. Enhanced traditional medicine should be given priority because of its potentials. This study have demonstrated feasibility of the green synthesis of F. sycomorus as a potent anti-shigellosis to combat the global burden of the disease. This is the first study On Stem bark aqueous extracts of F. sycomorus against Shigella species in the area.
| Published in | American Journal of Life Sciences (Volume 8, Issue 4) |
| DOI | 10.11648/j.ajls.20200804.16 |
| Page(s) | 82-90 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Nanoparticles, Shigellosis, Diarrhoeagenic, Damaturu, Ficus sycomorus, MDR
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APA Style
Yunusa Saheed, Ahmed Faruk Umar, Mahmud Yerima Iliyasu. (2020). Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens. American Journal of Life Sciences, 8(4), 82-90. https://doi.org/10.11648/j.ajls.20200804.16
ACS Style
Yunusa Saheed; Ahmed Faruk Umar; Mahmud Yerima Iliyasu. Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens. Am. J. Life Sci. 2020, 8(4), 82-90. doi: 10.11648/j.ajls.20200804.16
AMA Style
Yunusa Saheed, Ahmed Faruk Umar, Mahmud Yerima Iliyasu. Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens. Am J Life Sci. 2020;8(4):82-90. doi: 10.11648/j.ajls.20200804.16
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Download@article{10.11648/j.ajls.20200804.16,
author = {Yunusa Saheed and Ahmed Faruk Umar and Mahmud Yerima Iliyasu},
title = {Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens},
journal = {American Journal of Life Sciences},
volume = {8},
number = {4},
pages = {82-90},
doi = {10.11648/j.ajls.20200804.16},
url = {https://doi.org/10.11648/j.ajls.20200804.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20200804.16},
abstract = {Ficus sycomorus Plant was known traditionally for its medicinal properties, Shigella species as a bacterial was also known for their resistance to orthodox medicine. Hence the synthesis of silver nanoparticles from Ficus sycomorus. This study was carried out to investigate the anti-shigellosis potential of silver nanoparticles synthesized from Ficus sycomorus Linn stem bark aqueous extract against Multi-drug Resistant (MDR) Shigella species isolated from clinical specimen collected from patients attending Yobe State Specialist Hospital Damaturu, Nigeria. A total of 400 diarrhoeagenic stools were screened for isolation of Shigella species and determined their antibiotic susceptibility pattern using standard methods. Phytochemical constituents of Ficus sycomorus extract were used to synthesize silver nanoparticles using green synthesis approach. The nanoparticles was analyzed for transmittance, functional groups, sizes and shapes using Uv-vis, FTIR and Scanning Electron Microscopy (SEM), and was tested for antibacterial activities on MDR Shigella isolates. There is no significant difference in Shigella recovery relation to patients gender (P30 years (21). Shigella were also found to be sensitive to Ciprofloxacin (92%), Augmentin (87%), Cefuroxime (85%), Streptomycin (83.5%) while the most frequent resistance was showcased against Nalidixic Acid (48%), and Tetracycline (27%). Phytochemicals detected include saponins, flavonoids, alkaloids, cardiac glycoside and tannin. Uv-vis showed broad peaks around 460nm, the FTIR showed C-H stretch of hydroxyl group of alkanes and the SEM showed nanoparticles with wide range of shapes and sizes. Anti-Shigella activities of silver nanoparticles is higher at zones of inhibition between 10mm and 30mm higher compared to the activities of crude aqueous extract and AgNO3 solution against the MDR Shigella species which showed an enhanced activities. The high prevalence of shigellosis among children in this study, indicated that improved hygiene is needed for children in the area and detailed examination is required for the treatment of diarrhoea in adults. Ciprofloxacin and Amoxicillin Clavulanate, Nalidixic acid could be used only where culture and sensitivity results prevailed. Enhanced traditional medicine should be given priority because of its potentials. This study have demonstrated feasibility of the green synthesis of F. sycomorus as a potent anti-shigellosis to combat the global burden of the disease. This is the first study On Stem bark aqueous extracts of F. sycomorus against Shigella species in the area.},
year = {2020}
}
TY - JOUR T1 - Potential of Silver Nano Particles Synthesized from Ficus sycomorus Linn Against Multidrug Resistant Shigella species Isolated from Clinical Specimens AU - Yunusa Saheed AU - Ahmed Faruk Umar AU - Mahmud Yerima Iliyasu Y1 - 2020/08/25 PY - 2020 N1 - https://doi.org/10.11648/j.ajls.20200804.16 DO - 10.11648/j.ajls.20200804.16 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 82 EP - 90 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20200804.16 AB - Ficus sycomorus Plant was known traditionally for its medicinal properties, Shigella species as a bacterial was also known for their resistance to orthodox medicine. Hence the synthesis of silver nanoparticles from Ficus sycomorus. This study was carried out to investigate the anti-shigellosis potential of silver nanoparticles synthesized from Ficus sycomorus Linn stem bark aqueous extract against Multi-drug Resistant (MDR) Shigella species isolated from clinical specimen collected from patients attending Yobe State Specialist Hospital Damaturu, Nigeria. A total of 400 diarrhoeagenic stools were screened for isolation of Shigella species and determined their antibiotic susceptibility pattern using standard methods. Phytochemical constituents of Ficus sycomorus extract were used to synthesize silver nanoparticles using green synthesis approach. The nanoparticles was analyzed for transmittance, functional groups, sizes and shapes using Uv-vis, FTIR and Scanning Electron Microscopy (SEM), and was tested for antibacterial activities on MDR Shigella isolates. There is no significant difference in Shigella recovery relation to patients gender (P30 years (21). Shigella were also found to be sensitive to Ciprofloxacin (92%), Augmentin (87%), Cefuroxime (85%), Streptomycin (83.5%) while the most frequent resistance was showcased against Nalidixic Acid (48%), and Tetracycline (27%). Phytochemicals detected include saponins, flavonoids, alkaloids, cardiac glycoside and tannin. Uv-vis showed broad peaks around 460nm, the FTIR showed C-H stretch of hydroxyl group of alkanes and the SEM showed nanoparticles with wide range of shapes and sizes. Anti-Shigella activities of silver nanoparticles is higher at zones of inhibition between 10mm and 30mm higher compared to the activities of crude aqueous extract and AgNO3 solution against the MDR Shigella species which showed an enhanced activities. The high prevalence of shigellosis among children in this study, indicated that improved hygiene is needed for children in the area and detailed examination is required for the treatment of diarrhoea in adults. Ciprofloxacin and Amoxicillin Clavulanate, Nalidixic acid could be used only where culture and sensitivity results prevailed. Enhanced traditional medicine should be given priority because of its potentials. This study have demonstrated feasibility of the green synthesis of F. sycomorus as a potent anti-shigellosis to combat the global burden of the disease. This is the first study On Stem bark aqueous extracts of F. sycomorus against Shigella species in the area. VL - 8 IS - 4 ER -
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