Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia

Yongmei Li; Yubin Zhang; Jinfang Wang

doi:doi:10.11648/j.ajls.20200801.13

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Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia

Yongmei Li, Yubin Zhang, Jinfang Wang

Published in American Journal of Life Sciences (Volume 8, Issue 1)

Received: 26 May 2020 Published: 29 June 2020

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Abstract

Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.

Published in	American Journal of Life Sciences (Volume 8, Issue 1)
DOI	10.11648/j.ajls.20200801.13
Page(s)	14-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

HLA-G, Placenta, Preeclampsia, Pregnancy

References

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[4]	Warner CM, Lampton PW, Newmark JA, Cohen J. Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success. Reprod Biomed Online 2018; 17: 470-485.
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[14]	Tan CY, Chong YS, Loganath A, et al. Possible gene-gene interaction of KIR2DL4 with its cognate ligand HLA-G in modulating risk for preeclampsia. Reprod Sci 2019; 16: 1135-1143.
[15]	Moreau P, Contu L, Alba F, et al. HLA-G gene polymorphism in human placentas: possible association of G*0106 allele with preeclampsia and miscarriage. Biol Reprod 2018; 79: 459-467.
[16]	Zhou X, Zhang GY, Wang J, Lu SL, Cao J, Sun LZ. A novel bridge between oxidative stress and immunity: the interaction between hydrogen peroxide and human leukocyte antigen G in placental trophoblasts during preeclampsia. Am J Obstet Gynecol 2018; 206: 7-16.
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APA Style

Yongmei Li, Yubin Zhang, Jinfang Wang. (2020). Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. American Journal of Life Sciences, 8(1), 14-18. https://doi.org/10.11648/j.ajls.20200801.13

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ACS Style

Yongmei Li; Yubin Zhang; Jinfang Wang. Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. Am. J. Life Sci. 2020, 8(1), 14-18. doi: 10.11648/j.ajls.20200801.13

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AMA Style

Yongmei Li, Yubin Zhang, Jinfang Wang. Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. Am J Life Sci. 2020;8(1):14-18. doi: 10.11648/j.ajls.20200801.13

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@article{10.11648/j.ajls.20200801.13,
  author = {Yongmei Li and Yubin Zhang and Jinfang Wang},
  title = {Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia},
  journal = {American Journal of Life Sciences},
  volume = {8},
  number = {1},
  pages = {14-18},
  doi = {10.11648/j.ajls.20200801.13},
  url = {https://doi.org/10.11648/j.ajls.20200801.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20200801.13},
  abstract = {Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.},
 year = {2020}
}

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TY  - JOUR
T1  - Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia
AU  - Yongmei Li
AU  - Yubin Zhang
AU  - Jinfang Wang
Y1  - 2020/06/29
PY  - 2020
N1  - https://doi.org/10.11648/j.ajls.20200801.13
DO  - 10.11648/j.ajls.20200801.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 14
EP  - 18
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20200801.13
AB  - Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.
VL  - 8
IS  - 1
ER  -

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Author Information

Yongmei Li

Department of Gynecology, Binzhou People's Hospital, Binzhou, P.R. China
Yubin Zhang

Department of Nuclear Medicine, Binzhou People's Hospital, Binzhou, P.R. China
Jinfang Wang

Department of Gynecology, Binzhou People's Hospital, Binzhou, P.R. China

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APA Style

Yongmei Li, Yubin Zhang, Jinfang Wang. (2020). Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. American Journal of Life Sciences, 8(1), 14-18. https://doi.org/10.11648/j.ajls.20200801.13

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ACS Style

Yongmei Li; Yubin Zhang; Jinfang Wang. Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. Am. J. Life Sci. 2020, 8(1), 14-18. doi: 10.11648/j.ajls.20200801.13

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AMA Style

Yongmei Li, Yubin Zhang, Jinfang Wang. Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. Am J Life Sci. 2020;8(1):14-18. doi: 10.11648/j.ajls.20200801.13

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@article{10.11648/j.ajls.20200801.13,
  author = {Yongmei Li and Yubin Zhang and Jinfang Wang},
  title = {Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia},
  journal = {American Journal of Life Sciences},
  volume = {8},
  number = {1},
  pages = {14-18},
  doi = {10.11648/j.ajls.20200801.13},
  url = {https://doi.org/10.11648/j.ajls.20200801.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20200801.13},
  abstract = {Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.},
 year = {2020}
}

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TY  - JOUR
T1  - Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia
AU  - Yongmei Li
AU  - Yubin Zhang
AU  - Jinfang Wang
Y1  - 2020/06/29
PY  - 2020
N1  - https://doi.org/10.11648/j.ajls.20200801.13
DO  - 10.11648/j.ajls.20200801.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 14
EP  - 18
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20200801.13
AB  - Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.
VL  - 8
IS  - 1
ER  -

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