Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from <i>Detarium microcarpum</i> Guill. and Perr. (Caesalpiniaceae)

Ablassé Rouamba; Moussa Compaoré; Maurice Ouédraogo; Martin Kiendrebeogo

doi:doi:10.11648/j.ajbls.20180604.13

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Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae)

Ablassé Rouamba, Moussa Compaoré, Maurice Ouédraogo, Martin Kiendrebeogo

Published in American Journal of Biomedical and Life Sciences (Volume 6, Issue 4)

Received: 3 August 2018 Accepted: 31 August 2018 Published: 6 October 2018

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Abstract

DNA is continuously degraded by numerous genotoxic agents including intracellular reactive oxygen species produced by cell metabolism and exogenous environmental pollutants. These genotoxic agents destroy the DNA integrity leading to carcinogenesis or cell death. An increased consumption of vegetables, fruits and other foods rich in antioxidant compounds can protect DNA from oxidative damage and prevent cell carcinogenesis. This study was designed to investigate in vitro the genoprotective and DNA repair activities of the fruit pulp ethanol extract from Detarium microcarpum against two known mutagenic agents such as hydrogen peroxide and methyl methane sulfonate. To assess the genoprotective and DNA repair activities of extract, human lymphocytes in culture were treated with the extract before or after the genotoxic agent exposing. The amount of DNA damages was assessed by using the standard comet assay. The fruit pulp extract in concentration up to 500 µg/mL, compared to vehicle didn’t affect the integrity of DNA. Interestingly, the genotoxic effects of hydrogen peroxide and methyl methane sulfonate on human lymphocytes were significantly reduced by the extract pre-treatment. In addition, the DNA damages induced by hydrogen peroxide and methyl methane sulfonate were repaired further to the extract addition. The fruit pulp ethanol extract from Detarium microcarpum contains bioactive compounds that can preserve the integrity of DNA from the deleterious effects of genotoxic agents. The daily intake of this fruit pulp as food supplement could prevent DNA damages and carcinogenesis.

Published in	American Journal of Biomedical and Life Sciences (Volume 6, Issue 4)
DOI	10.11648/j.ajbls.20180604.13
Page(s)	78-84
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Detarium microcarpum, Genoprotection, DNA Repair, Hydrogen Peroxide, Methyl Methane Sulfonate

References

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APA Style

Ablassé Rouamba, Moussa Compaoré, Maurice Ouédraogo, Martin Kiendrebeogo. (2018). Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae). American Journal of Biomedical and Life Sciences, 6(4), 78-84. https://doi.org/10.11648/j.ajbls.20180604.13

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ACS Style

Ablassé Rouamba; Moussa Compaoré; Maurice Ouédraogo; Martin Kiendrebeogo. Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae). Am. J. Biomed. Life Sci. 2018, 6(4), 78-84. doi: 10.11648/j.ajbls.20180604.13

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AMA Style

Ablassé Rouamba, Moussa Compaoré, Maurice Ouédraogo, Martin Kiendrebeogo. Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae). Am J Biomed Life Sci. 2018;6(4):78-84. doi: 10.11648/j.ajbls.20180604.13

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@article{10.11648/j.ajbls.20180604.13,
  author = {Ablassé Rouamba and Moussa Compaoré and Maurice Ouédraogo and Martin Kiendrebeogo},
  title = {Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae)},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {6},
  number = {4},
  pages = {78-84},
  doi = {10.11648/j.ajbls.20180604.13},
  url = {https://doi.org/10.11648/j.ajbls.20180604.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20180604.13},
  abstract = {DNA is continuously degraded by numerous genotoxic agents including intracellular reactive oxygen species produced by cell metabolism and exogenous environmental pollutants. These genotoxic agents destroy the DNA integrity leading to carcinogenesis or cell death. An increased consumption of vegetables, fruits and other foods rich in antioxidant compounds can protect DNA from oxidative damage and prevent cell carcinogenesis. This study was designed to investigate in vitro the genoprotective and DNA repair activities of the fruit pulp ethanol extract from Detarium microcarpum against two known mutagenic agents such as hydrogen peroxide and methyl methane sulfonate. To assess the genoprotective and DNA repair activities of extract, human lymphocytes in culture were treated with the extract before or after the genotoxic agent exposing. The amount of DNA damages was assessed by using the standard comet assay. The fruit pulp extract in concentration up to 500 µg/mL, compared to vehicle didn’t affect the integrity of DNA. Interestingly, the genotoxic effects of hydrogen peroxide and methyl methane sulfonate on human lymphocytes were significantly reduced by the extract pre-treatment. In addition, the DNA damages induced by hydrogen peroxide and methyl methane sulfonate were repaired further to the extract addition. The fruit pulp ethanol extract from Detarium microcarpum contains bioactive compounds that can preserve the integrity of DNA from the deleterious effects of genotoxic agents. The daily intake of this fruit pulp as food supplement could prevent DNA damages and carcinogenesis.},
 year = {2018}
}

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TY - JOUR
T1 - Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae)
AU - Ablassé Rouamba
AU - Moussa Compaoré
AU - Maurice Ouédraogo
AU - Martin Kiendrebeogo
Y1 - 2018/10/06
PY - 2018
N1 - https://doi.org/10.11648/j.ajbls.20180604.13
DO - 10.11648/j.ajbls.20180604.13
T2 - American Journal of Biomedical and Life Sciences
JF - American Journal of Biomedical and Life Sciences
JO - American Journal of Biomedical and Life Sciences
SP - 78
EP - 84
PB - Science Publishing Group
SN - 2330-880X
UR - https://doi.org/10.11648/j.ajbls.20180604.13
AB - DNA is continuously degraded by numerous genotoxic agents including intracellular reactive oxygen species produced by cell metabolism and exogenous environmental pollutants. These genotoxic agents destroy the DNA integrity leading to carcinogenesis or cell death. An increased consumption of vegetables, fruits and other foods rich in antioxidant compounds can protect DNA from oxidative damage and prevent cell carcinogenesis. This study was designed to investigate in vitro the genoprotective and DNA repair activities of the fruit pulp ethanol extract from Detarium microcarpum against two known mutagenic agents such as hydrogen peroxide and methyl methane sulfonate. To assess the genoprotective and DNA repair activities of extract, human lymphocytes in culture were treated with the extract before or after the genotoxic agent exposing. The amount of DNA damages was assessed by using the standard comet assay. The fruit pulp extract in concentration up to 500 µg/mL, compared to vehicle didn’t affect the integrity of DNA. Interestingly, the genotoxic effects of hydrogen peroxide and methyl methane sulfonate on human lymphocytes were significantly reduced by the extract pre-treatment. In addition, the DNA damages induced by hydrogen peroxide and methyl methane sulfonate were repaired further to the extract addition. The fruit pulp ethanol extract from Detarium microcarpum contains bioactive compounds that can preserve the integrity of DNA from the deleterious effects of genotoxic agents. The daily intake of this fruit pulp as food supplement could prevent DNA damages and carcinogenesis.
VL - 6
IS - 4
ER -

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Author Information

Ablassé Rouamba

Laboratory of Applied Biochemistry and Chemistry, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Moussa Compaoré

Laboratory of Applied Biochemistry and Chemistry, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Maurice Ouédraogo

Laboratory of Animal Physiology, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Martin Kiendrebeogo

Laboratory of Applied Biochemistry and Chemistry, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso

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APA Style

Ablassé Rouamba, Moussa Compaoré, Maurice Ouédraogo, Martin Kiendrebeogo. (2018). Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae). American Journal of Biomedical and Life Sciences, 6(4), 78-84. https://doi.org/10.11648/j.ajbls.20180604.13

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ACS Style

Ablassé Rouamba; Moussa Compaoré; Maurice Ouédraogo; Martin Kiendrebeogo. Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae). Am. J. Biomed. Life Sci. 2018, 6(4), 78-84. doi: 10.11648/j.ajbls.20180604.13

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AMA Style

Ablassé Rouamba, Moussa Compaoré, Maurice Ouédraogo, Martin Kiendrebeogo. Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae). Am J Biomed Life Sci. 2018;6(4):78-84. doi: 10.11648/j.ajbls.20180604.13

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@article{10.11648/j.ajbls.20180604.13,
  author = {Ablassé Rouamba and Moussa Compaoré and Maurice Ouédraogo and Martin Kiendrebeogo},
  title = {Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae)},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {6},
  number = {4},
  pages = {78-84},
  doi = {10.11648/j.ajbls.20180604.13},
  url = {https://doi.org/10.11648/j.ajbls.20180604.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20180604.13},
  abstract = {DNA is continuously degraded by numerous genotoxic agents including intracellular reactive oxygen species produced by cell metabolism and exogenous environmental pollutants. These genotoxic agents destroy the DNA integrity leading to carcinogenesis or cell death. An increased consumption of vegetables, fruits and other foods rich in antioxidant compounds can protect DNA from oxidative damage and prevent cell carcinogenesis. This study was designed to investigate in vitro the genoprotective and DNA repair activities of the fruit pulp ethanol extract from Detarium microcarpum against two known mutagenic agents such as hydrogen peroxide and methyl methane sulfonate. To assess the genoprotective and DNA repair activities of extract, human lymphocytes in culture were treated with the extract before or after the genotoxic agent exposing. The amount of DNA damages was assessed by using the standard comet assay. The fruit pulp extract in concentration up to 500 µg/mL, compared to vehicle didn’t affect the integrity of DNA. Interestingly, the genotoxic effects of hydrogen peroxide and methyl methane sulfonate on human lymphocytes were significantly reduced by the extract pre-treatment. In addition, the DNA damages induced by hydrogen peroxide and methyl methane sulfonate were repaired further to the extract addition. The fruit pulp ethanol extract from Detarium microcarpum contains bioactive compounds that can preserve the integrity of DNA from the deleterious effects of genotoxic agents. The daily intake of this fruit pulp as food supplement could prevent DNA damages and carcinogenesis.},
 year = {2018}
}

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