In the past five years, the epidemic situation of brucellosis in Hohhot, Inner Mongolia, has been aggravated. The current diagnostic technology has low specificity. Through this topic, we can find a more convenient, safer, more specific and sensitive method for preparation. Better antigens are used for early rapid diagnosis of Brucellosis. Molecular biology techniques were used to design primers based on the published bp26 gene of Brucella from Gen-Bank. The DNA of Brucella isolates was extracted, PCR amplified and sequenced, and the bp26 gene sequence of the reference strain was identical to that of the reference strain. By comparing and analyzing the origins, the bp26 gene was amplified and compared with sheep, cattle, pigs, and sheep. In 2010, random surveys were conducted among 11401 people in 5331 farmers around Hohhot. The infection rate of Brucellosis was 3.82%, and 139059 of sheep were sampled in township and townships, and the positive rate was 2.82%. The results of bp26 gene sequence showed that the isolates of this experiment were 99.9% homologous to Brucella species 16M and Brucella species C68. The isolates were Brucella species 1320, Epididymis oryzae 280 and cattle. The homology of the Brucella strain S18 was 99.8%, 99.8%, and 98.9%, respectively. According to the phylogenetic tree analysis, the bp26 genes of Brucella strains of different strains are close, indicating that bp26 gene is very conservative. The homologous genes obtained by gene amplification technology can be used to detect br26 disease using bp26 protein. Other possible interferences can be ruled out and the diagnosis of Brucella can be promptly made. The culture and control of virulent strains of Brucella can be avoided. This experiment laid the foundation for the establishment of a new detection method using recombinant bp26 protein.
| Published in | American Journal of Biomedical and Life Sciences (Volume 6, Issue 3) |
| DOI | 10.11648/j.ajbls.20180603.15 |
| Page(s) | 63-68 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Brucellosis, Early Diagnosis, bp26 Gene, PCR
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APA Style
Xiaohui Wang, Dexi Li, Lidao Bao, Ruilian Ma, Sha Li. (2018). An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China. American Journal of Biomedical and Life Sciences, 6(3), 63-68. https://doi.org/10.11648/j.ajbls.20180603.15
ACS Style
Xiaohui Wang; Dexi Li; Lidao Bao; Ruilian Ma; Sha Li. An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China. Am. J. Biomed. Life Sci. 2018, 6(3), 63-68. doi: 10.11648/j.ajbls.20180603.15
AMA Style
Xiaohui Wang, Dexi Li, Lidao Bao, Ruilian Ma, Sha Li. An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China. Am J Biomed Life Sci. 2018;6(3):63-68. doi: 10.11648/j.ajbls.20180603.15
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Download@article{10.11648/j.ajbls.20180603.15,
author = {Xiaohui Wang and Dexi Li and Lidao Bao and Ruilian Ma and Sha Li},
title = {An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China},
journal = {American Journal of Biomedical and Life Sciences},
volume = {6},
number = {3},
pages = {63-68},
doi = {10.11648/j.ajbls.20180603.15},
url = {https://doi.org/10.11648/j.ajbls.20180603.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20180603.15},
abstract = {In the past five years, the epidemic situation of brucellosis in Hohhot, Inner Mongolia, has been aggravated. The current diagnostic technology has low specificity. Through this topic, we can find a more convenient, safer, more specific and sensitive method for preparation. Better antigens are used for early rapid diagnosis of Brucellosis. Molecular biology techniques were used to design primers based on the published bp26 gene of Brucella from Gen-Bank. The DNA of Brucella isolates was extracted, PCR amplified and sequenced, and the bp26 gene sequence of the reference strain was identical to that of the reference strain. By comparing and analyzing the origins, the bp26 gene was amplified and compared with sheep, cattle, pigs, and sheep. In 2010, random surveys were conducted among 11401 people in 5331 farmers around Hohhot. The infection rate of Brucellosis was 3.82%, and 139059 of sheep were sampled in township and townships, and the positive rate was 2.82%. The results of bp26 gene sequence showed that the isolates of this experiment were 99.9% homologous to Brucella species 16M and Brucella species C68. The isolates were Brucella species 1320, Epididymis oryzae 280 and cattle. The homology of the Brucella strain S18 was 99.8%, 99.8%, and 98.9%, respectively. According to the phylogenetic tree analysis, the bp26 genes of Brucella strains of different strains are close, indicating that bp26 gene is very conservative. The homologous genes obtained by gene amplification technology can be used to detect br26 disease using bp26 protein. Other possible interferences can be ruled out and the diagnosis of Brucella can be promptly made. The culture and control of virulent strains of Brucella can be avoided. This experiment laid the foundation for the establishment of a new detection method using recombinant bp26 protein.},
year = {2018}
}
TY - JOUR T1 - An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China AU - Xiaohui Wang AU - Dexi Li AU - Lidao Bao AU - Ruilian Ma AU - Sha Li Y1 - 2018/08/13 PY - 2018 N1 - https://doi.org/10.11648/j.ajbls.20180603.15 DO - 10.11648/j.ajbls.20180603.15 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 63 EP - 68 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20180603.15 AB - In the past five years, the epidemic situation of brucellosis in Hohhot, Inner Mongolia, has been aggravated. The current diagnostic technology has low specificity. Through this topic, we can find a more convenient, safer, more specific and sensitive method for preparation. Better antigens are used for early rapid diagnosis of Brucellosis. Molecular biology techniques were used to design primers based on the published bp26 gene of Brucella from Gen-Bank. The DNA of Brucella isolates was extracted, PCR amplified and sequenced, and the bp26 gene sequence of the reference strain was identical to that of the reference strain. By comparing and analyzing the origins, the bp26 gene was amplified and compared with sheep, cattle, pigs, and sheep. In 2010, random surveys were conducted among 11401 people in 5331 farmers around Hohhot. The infection rate of Brucellosis was 3.82%, and 139059 of sheep were sampled in township and townships, and the positive rate was 2.82%. The results of bp26 gene sequence showed that the isolates of this experiment were 99.9% homologous to Brucella species 16M and Brucella species C68. The isolates were Brucella species 1320, Epididymis oryzae 280 and cattle. The homology of the Brucella strain S18 was 99.8%, 99.8%, and 98.9%, respectively. According to the phylogenetic tree analysis, the bp26 genes of Brucella strains of different strains are close, indicating that bp26 gene is very conservative. The homologous genes obtained by gene amplification technology can be used to detect br26 disease using bp26 protein. Other possible interferences can be ruled out and the diagnosis of Brucella can be promptly made. The culture and control of virulent strains of Brucella can be avoided. This experiment laid the foundation for the establishment of a new detection method using recombinant bp26 protein. VL - 6 IS - 3 ER -
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