Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility

Irina Ivanovna Shilnikova; Irina Aleksandrovna Kluchnikova; Inna Vasilyevna Tereshchenko; Natalia Vladimirovna Dmitrieva

doi:doi:10.11648/j.ajbls.20170504.16

Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva

Published in American Journal of Biomedical and Life Sciences (Volume 5, Issue 4)

Received: 20 February 2017 Accepted: 16 March 2017 Published: 18 October 2017

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Abstract

We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.

Published in	American Journal of Biomedical and Life Sciences (Volume 5, Issue 4)
DOI	10.11648/j.ajbls.20170504.16
Page(s)	82-87
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Veillonella Clinical Isolates, Anaerobic Infections, MALDI-TOF MS, Antimicrobial Susceptibility, Resistance Rates, Cancer Patients

References

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[15]	Schmitt B. H., S. A. Cunningham, A. L. Dailey, D. R. Gustafson, R. Patel. Identification of anaerobic bacteria by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry with on-plate formic acid preparation. J. Clin. Microbiol., 2013, 51: 782-786.
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Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. (2017). Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. American Journal of Biomedical and Life Sciences, 5(4), 82-87. https://doi.org/10.11648/j.ajbls.20170504.16

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Irina Ivanovna Shilnikova; Irina Aleksandrovna Kluchnikova; Inna Vasilyevna Tereshchenko; Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am. J. Biomed. Life Sci. 2017, 5(4), 82-87. doi: 10.11648/j.ajbls.20170504.16

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Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am J Biomed Life Sci. 2017;5(4):82-87. doi: 10.11648/j.ajbls.20170504.16

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@article{10.11648/j.ajbls.20170504.16,
  author = {Irina Ivanovna Shilnikova and Irina Aleksandrovna Kluchnikova and Inna Vasilyevna Tereshchenko and Natalia Vladimirovna Dmitrieva},
  title = {Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {5},
  number = {4},
  pages = {82-87},
  doi = {10.11648/j.ajbls.20170504.16},
  url = {https://doi.org/10.11648/j.ajbls.20170504.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170504.16},
  abstract = {We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.},
 year = {2017}
}

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TY - JOUR
T1 - Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility
AU - Irina Ivanovna Shilnikova
AU - Irina Aleksandrovna Kluchnikova
AU - Inna Vasilyevna Tereshchenko
AU - Natalia Vladimirovna Dmitrieva
Y1 - 2017/10/18
PY - 2017
N1 - https://doi.org/10.11648/j.ajbls.20170504.16
DO - 10.11648/j.ajbls.20170504.16
T2 - American Journal of Biomedical and Life Sciences
JF - American Journal of Biomedical and Life Sciences
JO - American Journal of Biomedical and Life Sciences
SP - 82
EP - 87
PB - Science Publishing Group
SN - 2330-880X
UR - https://doi.org/10.11648/j.ajbls.20170504.16
AB - We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.
VL - 5
IS - 4
ER -

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Author Information

Irina Ivanovna Shilnikova

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation
Irina Aleksandrovna Kluchnikova

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation
Inna Vasilyevna Tereshchenko

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation
Natalia Vladimirovna Dmitrieva

Department of Health, N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow, Russian Federation

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APA Style

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. (2017). Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. American Journal of Biomedical and Life Sciences, 5(4), 82-87. https://doi.org/10.11648/j.ajbls.20170504.16

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Irina Ivanovna Shilnikova; Irina Aleksandrovna Kluchnikova; Inna Vasilyevna Tereshchenko; Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am. J. Biomed. Life Sci. 2017, 5(4), 82-87. doi: 10.11648/j.ajbls.20170504.16

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AMA Style

Irina Ivanovna Shilnikova, Irina Aleksandrovna Kluchnikova, Inna Vasilyevna Tereshchenko, Natalia Vladimirovna Dmitrieva. Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility. Am J Biomed Life Sci. 2017;5(4):82-87. doi: 10.11648/j.ajbls.20170504.16

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@article{10.11648/j.ajbls.20170504.16,
  author = {Irina Ivanovna Shilnikova and Irina Aleksandrovna Kluchnikova and Inna Vasilyevna Tereshchenko and Natalia Vladimirovna Dmitrieva},
  title = {Species Identification of Clinical Veillonella Isolates by MALDI-TOF Mass Spectrometry and Evaluation of Their Antimicrobial Susceptibility},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {5},
  number = {4},
  pages = {82-87},
  doi = {10.11648/j.ajbls.20170504.16},
  url = {https://doi.org/10.11648/j.ajbls.20170504.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170504.16},
  abstract = {We investigated the possibilities of the MALDI-TOF MS for species identification of anaerobic gram-negative cocci isolated from clinical specimens of cancer patients. A total 70 Veillonella clinical isolates and one Acidaminococcus intestini isolate were analysed by the Bruker Microflex MALDI-TOF instrument with the Biotyper 3, 0 software. All isolates were identified to the species level with a scores greater than 1.9. The most common species were V. parvula (37 strains), then followed by decreasing the frequency V. dispar (16), V. atypica (16) and V. denticariosi (1). Susceptibilities of the isolates were determined by the E-test methodology. All Veillonella isolates were susceptible to imipenem, whereas a high resistance rates were observed for penicillin G, amoxicillin/clavulanate and metronidazole. The proportion of intermediate/resistant isolates of V. parvula, V. dispar and V. atypica to penicillin (MIC ≥ 1 µg/ml) was 86%, 85% and 100%, respectively. The resistance to amoxicillin/clavulanate (MIC 16 - 32 µg/ml) was observed in about 28,6% V. parvula isolates, 23,1% V. dispar isolates and 6,7% V. atypica isolates. According to EUCAST criteria, resistance to metronidazole (MIC ≥ 8 µg/ml) of V. parvula, V. dispar and V. atypica was 88,6%, 53,8% and 40%, respectively.},
 year = {2017}
}

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