Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)

Luu Thi Ngoc Huyen; Luu Minh Cuc; Le Huy Ham; Tran Dang Khanh

doi:doi:10.11648/j.ajbio.20130104.15

| Peer-Reviewed

Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh

Published in American Journal of BioScience (Volume 1, Issue 4)

Received: 1 October 2013 Published: 30 November 2013

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.

Published in	American Journal of BioScience (Volume 1, Issue 4)
DOI	10.11648/j.ajbio.20130104.15
Page(s)	80-84
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Q5DB, Marker Assisted Selection, Rice, Saltol- QTL

References

[1]	Munns, R., and Tester, M. Mechanisms of salinity tolerance. Ann Rev Plant Biol., 2008; 59: 651-681.
[2]	Nazar, R., Iqbal, N., Masood A., Syeed, S., and Khan, N. A. Understanding the signiﬁcance of sulfur in improving salinity tolerance in plants. Environ Exp Bot., 2011; 70: 80–87.
[3]	Khanh, T.D., L.H. Linh., T.H. Linh., L.H. Ham., T.D. Xuan. Rapid and high-precision marker assisted backcrossing to introgress the SUB1 QTL into the Vietnamese elite rice variety. J. Plant Bred Crop Sci., 2013; 5:26-33.
[4]	IRRI. The IRRI webpage: http://irri.org/index.php?option=com_(latest accession: May 25, 2013).
[5]	Nguyen, M.H., Thu, P.V., Cuong, N.T.. Evaluation arable soil composition in order to well manage the soil resource of Vietnam. In: Proceedings of the 9th Workshop of Vietnam, Institute of Meteorology Hydrology and Environment., 2006; 9:437-422.
[6]	Linh, L.H., Linh, T.H., Xuan, T.D., Ham, L.H., Ismail, A.M., Khanh, T.D.. Molecular breeding to improve salt tolerance of rice (Oryza sativa L.) in the Red River Delta of Vietnam. Inter J Plant Genom., 2012; ID 949039, 9 pages.
[7]	MARD (Ministry of Agriculture and Rural Development). Vietnam News Agency report., 2005.
[8]	Singh, D., Kumar, A., Kumar, A., Chauhan, P., Kumar, V., Kumar, N., Singh, A., Mahajan, N., Irohi, P., Chand, S., Ramesh, B., Shin, J., Kumar, P., Kumar, R., Yadav, R..B., Naresh, R.K. Marker assisted selection and crop management for salt tolerance: a review. Afr J Biotech., 2011; 10:14694-14698.
[9]	Jena, K.K., Mackill, D.J. Molecular markers and their use in marker assisted selection in rice. Crop Sci., 2008; 48:1266-1276.
[10]	Ben-Ari, G., Lavi, U. Marker assisted selection in plant breeding. In: Plant Biotechnology and Agriculture: Prospects for the 21st century. Altman, A, Michae, P., Lasegawa., (eds). 2012; 163-184.
[11]	Thomson, M.J,, Ismail, A.M., McCouch, S.R., Mackill, M.J. Marker assisted breeding. In: Pareek, A., Sopory, SK, Bohnert HJ, Govindjee., (eds). Abiotic stress adaptation in plants: physiological, molecular and genomic foundation. New York: Springer., 2010; pp. 451–69.
[12]	Septiningsih, E.M., Pamplona, A.M., Sanchez, D.L., Neeraja, C.N., Vergar, G.V., Heuer, S., Ismail, A.M., Mackill, D.J. Development of submergence tolerant rice cultivars: the Sub1 locus and beyond. Ann Bot., 2009;103: 151-160.
[13]	Singh, R.K,, Redoña, E.D., Refuerzo, L. Varietal improvement for abiotic stress tolerance in crop plants: special reference to salinity in rice. In: Pareek A, Sopory S.K, Bohnert H.J, Govindjee., (eds). Abiotic stress adaptation in plants: physiological, molecular and genomic foundation. New York: Springer., 2010; pp. 387–415.
[14]	Bonilla, P., Dvorak, J., Mackill, D., Deal, K., and Gregorio, G. RFLP and SSLP mapping of salinity tolerance genes in chromosome 1 of rice (Oryza sativa L.) using recombinant inbred line. Philip Agric Sci., 2002; 54:68-76.
[15]	Ren, Z.H., Gao, J.P., Li, L.G., Cai, X.L., Huang, W., Chao, D.Y., Zhu, M.Z., Wang, Z.Y., Luan, S., Lin, H.X. A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nature., 2005; 37:1141-1146.
[16]	Takehisa, H., Shimodate, T., Fukuta, Y., Ueda, T., Yano, Y., Yamaya, T., Kameya, T., Sato, T. Identification of quantitative trait loci for plant growth of rice in paddy field flooded with salt water. Field Crops Res., 2004; 89:85-95.
[17]	Islam, M.R., Gregorio, G.B. Progress of salinity tolerance rice variety development in Bangladesh. Sabrao J Bred Genet., 2013; 45:21-30.
[18]	Van Berloo, R.. GGT 2.0: versatile software for visualization and analysis of genetic data. J Hered., 2008; 99:232–236.
[19]	Yoshida, S., Forno, D.A., Cock, J.K., Gomez, K.A. Laboratory manual for physiological studies of rice. Manila: International Rice Research Institute; 1976. p. 38.
[20]	IRRI. Standard evaluation system for rice. 4th ed. Manila: International Rice Research Institute; 1996. p. 52
[21]	Gregorio, G.B., Aliyu, R., Adamu, A.K., Muazu, S., Alonge, S.O. Tagging and Validation of SSR markers to salinity tolerance QTLs in rice (Oryza spp). International conference on biology, environment and chemistry IPCBEE vol 1, IACSIT Press, Singapore, 2010; pp. 328-332.
[22]	Mir, R.R., Varshney, R.K.. Future prospects of molecular markers in plants. In: Molecular Markers in plants. Henry R.J., ed 1st, John Wiley & Sons, Inc. Publisher,. 2013; pp. 169-184.
[23]	Niones, J.M. Fine mapping of the salinity tolerance gene on chromosome 1 of rice (Oryza sativa L.) using near- isogenic lines. MS dissertation. Laguna: University of the Philippines Los Baños., 2004: pp.78.
[24]	Huyen, L.T.N., Cuc, L.M., Ismail, A.M., Ham, L.H. Introgressiong the salinity tolerance QTLs Saltol into AS996, the elite rice variety of Vietnam. Am J Plant Sci., 2012; 3:891-987.
[25]	Hien, V.T.T., Le, D.D., Ismail, A.M., Ham, L.H. Marker assisted backcrossing (MABC) for improved salinity tolerance in rice (Oryza sativa L.) to cope with climate change in Vietnam. Aust J Crop Sci., 2012, 6:1649-1654.

Cite This Article

Plain Text BibTeX RIS

APA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. (2013). Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). American Journal of BioScience, 1(4), 80-84. https://doi.org/10.11648/j.ajbio.20130104.15

Copy | Download

ACS Style

Luu Thi Ngoc Huyen; Luu Minh Cuc; Le Huy Ham; Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am. J. BioScience 2013, 1(4), 80-84. doi: 10.11648/j.ajbio.20130104.15

Copy | Download

AMA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am J BioScience. 2013;1(4):80-84. doi: 10.11648/j.ajbio.20130104.15

Copy | Download

@article{10.11648/j.ajbio.20130104.15,
  author = {Luu Thi Ngoc Huyen and Luu Minh Cuc and Le Huy Ham and Tran Dang Khanh},
  title = {Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {4},
  pages = {80-84},
  doi = {10.11648/j.ajbio.20130104.15},
  url = {https://doi.org/10.11648/j.ajbio.20130104.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130104.15},
  abstract = {Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)
AU  - Luu Thi Ngoc Huyen
AU  - Luu Minh Cuc
AU  - Le Huy Ham
AU  - Tran Dang Khanh
Y1  - 2013/11/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130104.15
DO  - 10.11648/j.ajbio.20130104.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 80
EP  - 84
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130104.15
AB  - Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.
VL  - 1
IS  - 4
ER  -

Copy | Download

Author Information

Luu Thi Ngoc Huyen

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Luu Minh Cuc

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Le Huy Ham

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam
Tran Dang Khanh

Molecular Biology Division, Agricultural Genetics Institute, Pham Van Dong Road, Tuliem, Hanoi, Vietnam

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. (2013). Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). American Journal of BioScience, 1(4), 80-84. https://doi.org/10.11648/j.ajbio.20130104.15

Copy | Download

ACS Style

Luu Thi Ngoc Huyen; Luu Minh Cuc; Le Huy Ham; Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am. J. BioScience 2013, 1(4), 80-84. doi: 10.11648/j.ajbio.20130104.15

Copy | Download

AMA Style

Luu Thi Ngoc Huyen, Luu Minh Cuc, Le Huy Ham, Tran Dang Khanh. Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS). Am J BioScience. 2013;1(4):80-84. doi: 10.11648/j.ajbio.20130104.15

Copy | Download

@article{10.11648/j.ajbio.20130104.15,
  author = {Luu Thi Ngoc Huyen and Luu Minh Cuc and Le Huy Ham and Tran Dang Khanh},
  title = {Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {4},
  pages = {80-84},
  doi = {10.11648/j.ajbio.20130104.15},
  url = {https://doi.org/10.11648/j.ajbio.20130104.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130104.15},
  abstract = {Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Introgression the SALTOL QTL into Q5DB, the Elite Variety of Vietnam Using Marker- Assisted - Selection (MAS)
AU  - Luu Thi Ngoc Huyen
AU  - Luu Minh Cuc
AU  - Le Huy Ham
AU  - Tran Dang Khanh
Y1  - 2013/11/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130104.15
DO  - 10.11648/j.ajbio.20130104.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 80
EP  - 84
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130104.15
AB  - Rice is a main food crop in Vietnam and plays a key role in economic growth in this country. However, its production and cultivating areas are severely affected from the threats of devastation caused by the rise of sea level. In this study, we have focused on developing new rice lines with salinity tolerance and high yield by applying markers assisted selection (MAS). Total of 21 primers in the Saltol QTL region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol QTL region of the breeding populations. The individual plants in BC1, BC2, and BC3 generations of the Q5DB/FL478 were analyzed to evaluate the introgression of Saltol fragment into Q5DB cultivar. After screening of 3 BC generations, the best individual plants of BC3F1 of the plant numbers QF-3-1, QF-3-2, QF4-3-3, QF6-3 with almost the recipient alleles were selected. Conventional breeding was developed on BC3F2 for selection of the new salt tolerance rice lines with high yield, resistance to the biotic stress. The salinity tolerance of the new lines was also performed using the standard system of screening.
VL  - 1
IS  - 4
ER  -

Copy | Download