Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology

Md. Sakil Munna; Ifra Tun NUR; Tasmina Rahman; Rashed Noor

doi:doi:10.11648/j.ajbio.20130104.12

| Peer-Reviewed

Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology

Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor

Published in American Journal of BioScience (Volume 1, Issue 4)

Received: 23 October 2013 Published: 10 November 2013

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.

Published in	American Journal of BioScience (Volume 1, Issue 4)
DOI	10.11648/j.ajbio.20130104.12
Page(s)	59-62
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Escherichia Coli, Oxidative Stress, Hydrogen Peroxide (H2O2), Cell Culturability, Cell Death

References

[1]	Den Besten HMW., Effraimidou S., Abee T. (2013) Catalase Activity as a Biomarker for Mild-Stress-Induced Robustness in Bacillus weihenstephanensis. Appl. Environ. Microbiol. 79(1):57-62.
[2]	Den Besten HMW., Mols M., Moezelaar R., Zwietering MH., Abee T. (2009) Phenotypic and transcriptomic analyses of mildly and severely salt-stressed Bacillus cereus ATCC 14579 cells. Appl. Environ. Microbiol.75:4111–4119.
[3]	Den Besten HMW., Mataragas M., Moezelaar R., Abee T., Zwietering MH. (2006) Quantification of the effects of salt stress and physiological state on thermotolerance of Bacillus cereus ATCC 10987 and ATCC 14579. Appl. Environ. Microbiol. 72:5884–5894.
[4]	Nagamitsu H., Murata M, Kosaka T., Kawaguchi J., Mori H., Yamada M., (2013) Crucial roles of MicA and RybB as vital factors for E Dependent cell lysis in Escherichia coli long-term stationary phase. J Mol Microbiol Biotechnol. 23:227–232
[5]	Huillet E., Tempelars MH., Leroux GA., Wanapaisan P., Bridoux L., Makhzamis S. (2012) PIcRa. a new qurum-sensing regulator from Bacillus cereus, play a role in oxidative stress response and cystein metabolism in stationary phase. Genetigue Microbienne et Environment. 7(12): e51047.
[6]	Murata M., Noor R., Nagamitsu H., Tanaka S., Yamada M. (2012) Novel pathway directed by E to cause cell lysis in Escherichia coli. Genes Cells. 17:234-247.
[7]	Pohl S., Tu W., Aldridge P., Gillespie C., Hahne H., Mader U. (2011) Combined proteomic and transcriptomic analysis of the response of Bacillus anthracis to oxidative stress. Proteomics. 11(15):3036-55.
[8]	Noor R., Murata M., Yamada M. (2009a) Oxidative stress as a trigger for growth phase-specific E dependent cell lysis in Escherichia coli. J Mol Microbiol Biotechnol.17:177-187.
[9]	Noor R., Murata M., Nagamitsu H., Klein G., Raina S., Yamada M. (2009b) Dissection of E dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst Ppid. Genes Cells.14:885-899.
[10]	Passalacqua K., Bergman, N., Leejy., Sherman, D., Hanna, P. (2007) The global transcriptional responses of Bacillus anthracis sterne (34F2) and a delta sodAI mutant to paraquat reveal metal ion homeostasis inbalances during endogenous superoxide stress. J Bacteriol. 189 (11):3996-4013.
[11]	Passalacqua K., Bergman N., Leejy Sherman D., Hanna P. (2006) The superoxide dismutases of Bacillus antracis do not cooperatively protect against endogenous superoxide stress. J Bacteriol. 188(11):3837-48.
[12]	Cuny C., Dukan L., Fraysse L., Ballesteros M., Dukan S. (2005) Investigations of the first events leading to the loss of culturability during Escherichia coli starvation: future nonculturable bacteria form a subpopulation. J Bacteriol. 187:2244–2248.
[13]	Kabir MS., Yamashita D., Noor R., Yamada M. (2004) Effect of S on E -Directed Cell Lysis in Escherichia coli Early Stationary Phase. J Mol Microbiol Biotechnol. 8:189–194.
[14]	Chang W.S., Halverson LJ. (2003) Reduced water availability influences the dynamics, development, and ultrastructural properties of Pseudomonas putidabiofilms. J. Bacteriol. 185:6199–6204.
[15]	Sabra Eun-Jin K., An-ping Z. (2002) Physiological response of Pseudomonas aeruginosa PA01 to oxidative stress in controlled microaerobic and aerobic cultures. Microbiology. 148:3195-3202.
[16]	Givskov M., Eberl L., Moller S., Poulsen LK., Molin S. (1994) Responses to nutrient starvation in Pseudomonas Putida KT2442: analysis of general cross-protection, cell shape, and macromolecular content. J Bacteriol. 176:7-14.
[17]	Inaoka, T., Matsumura, Y., Tsuchido, T. (1990) SodA and manganese are essential for resistance to oxidative stress in growing and sporulating cells of Bacillus subtilis. J Bacteriol. 181(6):1939-43.
[18]	Nystrom T. (2005). Role of oxidative carbonylation in protein quality control and senescence The EMBO Journal. 24:1311–1317.
[19]	Desnues B., Cuny C., Gregori G., Dukan S., Aguilaniu H., Nystrom T. (2003) Differential oxidative damage and expression of stress defence regulons in culturable and nonculturable Escherichia coli cells. EMBO reports 4:400-404.
[20]	Dukan S., Nystrom T. (1999) Oxidative stress defense and deterioration of growth arrested Escherichia coli cells. J Biol Chem. 274: 26027–26032.
[21]	Farr SB., Kogoma T. (1991) Oxidative Stress Responses in Escherichia coli and Salmonella typhimurium. American Society for Microbiology. 55(4):561-585.
[22]	Puglia C., Powell S. (1984) Inhibition of cellular antioxidants: a possible mechanism of toxic cell injury. Environ. Health Perspect.57:307–11.
[23]	Nitta T., Nagamitsu H., Murata M., Izu H., Yamada M. (2000) Function of the sigma-E regulon in dead-cell lysis in stationary phase Escherichia coli. J Bacteriol. 182:5231–5237.
[24]	Noor R., Islam Z., Munshi SK., Rahman F. (2013) Influence of Temperature on Escherichia coli Growth in Different Culture Media. Journal of pure and applied microbiology. 7(2):899-904.
[25]	Yamada M., Noor R., Nagamitsu H., Murta M. (2009) The higher temperature, the more oxidative stress and lysis in Escherichia coli. The 3rd International Conference on Fermentation Technology for Value Added Agricultural Products. Khon Kaen, Thailand. FerVAAP: ACP 09.
[26]	Baez A., Shiloach J. (2013) Escherichia coli avoids high dissolved oxygen stress by activation of SoxRS and manganese-superoxide dismutase. Microb Cell Fact. 12:12-23.
[27]	Chiang SM., Schellhorn HE. (2012) Regulators of oxidative stress response genes in Escherichia coli and their functional conservation in bacteria. Arch Biochem Biophys. 525(2):161-169.
[28]	Dai Y., Outte FW. (2012) The E. coli SufS-SufE sulfur transfer system is more resistant to oxidative stress than IscS-IscU. FEBS Lett. 586(22):4016-22.

Cite This Article

Plain Text BibTeX RIS

APA Style

Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor. (2013). Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. American Journal of BioScience, 1(4), 59-62. https://doi.org/10.11648/j.ajbio.20130104.12

Copy | Download

ACS Style

Md. Sakil Munna; Ifra Tun NUR; Tasmina Rahman; Rashed Noor. Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. Am. J. BioScience 2013, 1(4), 59-62. doi: 10.11648/j.ajbio.20130104.12

Copy | Download

AMA Style

Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor. Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. Am J BioScience. 2013;1(4):59-62. doi: 10.11648/j.ajbio.20130104.12

Copy | Download

@article{10.11648/j.ajbio.20130104.12,
  author = {Md. Sakil Munna and Ifra Tun NUR and Tasmina Rahman and Rashed Noor},
  title = {Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {4},
  pages = {59-62},
  doi = {10.11648/j.ajbio.20130104.12},
  url = {https://doi.org/10.11648/j.ajbio.20130104.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130104.12},
  abstract = {The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology
AU  - Md. Sakil Munna
AU  - Ifra Tun NUR
AU  - Tasmina Rahman
AU  - Rashed Noor
Y1  - 2013/11/10
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130104.12
DO  - 10.11648/j.ajbio.20130104.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 59
EP  - 62
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130104.12
AB  - The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.
VL  - 1
IS  - 4
ER  -

Copy | Download

Author Information

Md. Sakil Munna

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Ifra Tun NUR

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Tasmina Rahman

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor. (2013). Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. American Journal of BioScience, 1(4), 59-62. https://doi.org/10.11648/j.ajbio.20130104.12

Copy | Download

ACS Style

Md. Sakil Munna; Ifra Tun NUR; Tasmina Rahman; Rashed Noor. Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. Am. J. BioScience 2013, 1(4), 59-62. doi: 10.11648/j.ajbio.20130104.12

Copy | Download

AMA Style

Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor. Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. Am J BioScience. 2013;1(4):59-62. doi: 10.11648/j.ajbio.20130104.12

Copy | Download

@article{10.11648/j.ajbio.20130104.12,
  author = {Md. Sakil Munna and Ifra Tun NUR and Tasmina Rahman and Rashed Noor},
  title = {Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology},
  journal = {American Journal of BioScience},
  volume = {1},
  number = {4},
  pages = {59-62},
  doi = {10.11648/j.ajbio.20130104.12},
  url = {https://doi.org/10.11648/j.ajbio.20130104.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130104.12},
  abstract = {The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology
AU  - Md. Sakil Munna
AU  - Ifra Tun NUR
AU  - Tasmina Rahman
AU  - Rashed Noor
Y1  - 2013/11/10
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbio.20130104.12
DO  - 10.11648/j.ajbio.20130104.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 59
EP  - 62
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20130104.12
AB  - The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.
VL  - 1
IS  - 4
ER  -

Copy | Download