In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia

Muh Jayadi; Baharuddin; Bachrul Ibrahim

doi:doi:10.11648/j.ajaf.20130104.14

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In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia

Muh Jayadi, Baharuddin, Bachrul Ibrahim

Published in American Journal of Agriculture and Forestry (Volume 1, Issue 4)

Received: 18 November 2013 Published: 20 December 2013

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Abstract

Phosphorus (P) is the second most required nutrient of plants, but its availability in ultisols is relatively low. Source of P can be found from rock phosphate (RP) which has low solubility level. Use of microbial solubilizing phosphate such as bacteria, fungi or combination of both is potential to increase availability of P. This research was aimed to find bacterial or fungal isolates that are high potential in solubilizing of RP. In vitro selection was conducted to obtain indigenous bacteria or fungi from Ultisols of South Sulawesi. Selection of microbes for their capability to solubate rock phosphate qualitatively, conducted through measuring the solubility index (SI) and solubility efficiency (SE) on pykovskaya solid medium and on National Botanical Research Institutes phosphate-bromophenol blue (NBRIP-BPB) medium. The results of this study indicated that from SI and SE highest values, four high potential isolates were selected namely, Aspergillus niger JTM 2, Bacillus sp JTM 3, Aspergillus niger JTM 6 and Pseudomonas sp JTM 10. Ability of solubilizing phosphate from the four isolates was then tested in liquid medium pikovskaya either in single or mixed combination. Solubilizing phosphate of RF was determined according to [1] method. The results of test on solid media indicate that Bacillus sp JTM 3 and Pseudomonas sp JTM 10 have SI and SE higher than both Aspergillus niger JTM 2 and Aspergillus niger JTM 6. Futhermore, test on Pikovskaya liquid media confirmed that Aspergillus niger JTM 6 and Pseudomonas sp JTM 10 gives the higher results than other single isolates and control, whereas the combination of isolates Bacillus sp JTM 3 and Aspergillus niger JTM 2 indicated significantly higher solubilizing phosphorus production than all other treatments.

Published in	American Journal of Agriculture and Forestry (Volume 1, Issue 4)
DOI	10.11648/j.ajaf.20130104.14
Page(s)	68-73
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

In Vitro, Mixture Isolates, Pikovskaya Media, Rock Phosphate, Ultisols

References

[1]	J. Murphy, And J. R. Riley, A Modified solution method for determination of phosphate in natural water, vol. 27. Analytical Chimica Acta, 1962, pp. 31-36.
[2]	A. Mulyani, A. Rachman and A. Dairah," Penyebaran lahan masam, Potensi dan ketersediaannya untuk pengembangan pertanian," in Pemanfaatan fosfat alam sebagai sumber pupuk P. Balai Penelitian Tanah. Puslittanak, Bogor, 2009, pp, 23 – 44.
[3]	J. Adiningsih dan M. Sudjadi, "Peranan sistem bertanam lorong (Alley cropping) dalam meningkatkan kesuburan tanah pada lahan kering masam." Risalah Seminar Hasil Penelitian Tanah dan Agroklimat. Pusat Penelitian Tanah dan Agroklimat, Bogor. 1993, unpublished.
[4]	H. G. Soepardi,"Strategi usahatani agribisnis berbasis sumber daya lahan", in Prosiding Nasional Pengelolaan Sumber daya Lahan dan Pupuk Buku I. Pusat Penelitian dan Pengembangan Tanah dan Agroklimat, Bogor. 2001, pp. 35-52
[5]	H. M. Hosseini, S. Kayami, H. Besharati and S. Bybordi, "Study of the effects of rock phosphate application with phosphate solubilizing bacteria on P availability for corn," world congress of Soil Science, Soil Solutions for Changing World, August 2010.
[6]	K. F. Isherword, "Fertilizer use and environment". In Proc. Symp. Plant Nutrition Management for Sustainable Agricultural Growth.. N. Ahmed and A. Hamid (eds.), Islamabad: NFDC, 1998. pp. 57-76.
[7]	J.K Vessey, K. Pawlowski and B. Bergman, Root-based N2-fixing symbioses: Legumes, actinorhizal plants, Parasponia sp., and cycads, vol. 266. Plant and Soil, 2004, pp. 205 – 230.
[8]	H, N. Fankem, N. Laurette, D. Annette, Q. John, M. Wolfgang, E. François-Xavier., and N. Dieudonné. Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluorescens isolated from acidic soils of Cameroon, vol. 2. African J. Microbiol. Res, 2008, pp. 171-178.
[9]	E. Premono, M. A. Moawad and P. L. G. Vleck, Effect of phosphate solubilizing Pesudomonas putida on the growth of maize and its survival in the rhizosphere, vol. 11. Indonesian Journal of Crop Science, 1996, pp. 13-23.
[10]	J. M. Igual, A. Valverde, E. Cervantes and E. Velázquez.. Phosphate-solubilizing bacteria as inoculants for agriculture: use of updated molecular techniques in their study, vol. 21. Agronomie, 2001, pp. 561-568.
[11]	I. Anas, E. Premono dan R. Widyastuti. "Peningkatan Efisiensi Pemupukan P dengan Menggunakan Mikroorganisme Pelarut P." Pusat antar Universitas IPB. Bogor. 1993..unpublished.
[12]	P. Lestari, "Pengaruh fungi pelarut fosfat terhadap serapan hara P dan Peertumbuhan Tanaman Jagung." Skripsi. Fakultas Pertanian IPB. Bogor. 1994. unpublished.
[13]	M. Rashid, S. Khalil, N. Ayub, S. Alam and F. Latif. Organic acids production and phosphate solubilizing microorganisms (SM) under invitro condition, vol.7 (2). Pakistan Journal of Biological Sciences, 2004. pp. 187 – 196.
[14]	P. Illmer, and F.Schinner, Solubilization of inorganic phosphate by microorganisms isolated from forest soils., vol 24. Soil Biol. Biochem, 1992, pp. 389-395.
[15]	P. E. A. Asea, R. M. N. Kucey and J. W. B. Stewart. Inorganic phosphate solubilization by two Penicillium species in solution culture and soil, vol. 20. Soil Biol. Biochem, 1988, pp. 459 – 464.
[16]	H. M. Salih, A. I. Yahya, A. M. Abdul-Rahman and B. H. Munam. Avallability of phosphorus in a calcareous soil treated with rock phosphate or super phosphate or effected by phosphate dissolving fungi, vol. 20. Plant soil, 1989, pp. 181 – 185.
[17]	M. A. Whitelaw, T.J. Harden and K.R. Helyar, Phosphate solubilization in solution culture by the soil fungus Penicillium radicum, vol. 31. Soil Biol. Biochem, 1999, pp. 655–665.
[18]	V. Narsian, Takkar, J. and Patel, H.H. Mineral phosphate solubilization by Aspergillus aculeatus. Indian J. Expt. Biol, 1995, pp. 33, 91.
[19]	D. H. Goenadi, and R. Saraswati. Kemampuan melarutkan fosfat dari beberapa isolat fungi pelarut fosfat, vol, 61(3). Menara Perkebunan, 1993, pp. 61 – 66.
[20]	Alam S., S. Khalil, N. Ayub and M. Rashid. In vitro solubilization of inorganic phosphate by phosphate solubilizing microorganism (PSM) from maize rhizosphere, vol. 4. No. 4. Int. J. Agric. Biol, 2002, pp. 454-458.
[21]	Z. L. He, W. Bian and J. Zhu. Screening and identification of microorganisms capable of utilizing phosphate adsorbed by goethite, vol. 33. Comm. Soil Sci. Plant Anal, 2002, pp. 647-663.
[22]	Y. Sastro, D. Widianto,and D. Shiddieq. Secretion of organic acids by Aspergillus niger YD 17 that grown with rock phosphate, vol. XI (3). Biota, 2006, pp. 167 – 175.
[23]	Y.P. Chen, P. D. Rekha., A. B. Arun., F. T. Shen., W. A. Lai., and C. C. Young, Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities, vol. 34. Applied Soil Ecology, 2006, pp. 33 – 41.
[24]	N.S.. Subba Rao, "Biofertilizer in Agricultural," Oxford and IBH Publishing Co. New Delhi. Bombai. 1982, pp. 186.
[25]	C. S. Nautiyal, S. Mehta and P. Pushpangadan. "Composition for Qualitative Screening of Phosphate Solubilizing Microorganisms and a Qualitative Method for Screening Microorganisms." Council of Scientific and Industrial Research, New Delhi. October 2003.
[26]	O. J. Chibuogwu and E. H. Nmesoma,. Bath culture studies of Phosphate solubilisation by Micrococcus sp PSB 7 isolated from rhizospheric soil, vol. 10 (4). American-Eurasian J. Agric and Environ. Sci, 2011, pp. 667-674
[27]	K. Sharma, G. Dak, A. Agrawal, M. Bhatnagar and R. Sharmar, Effect of phosphate solubilizing bacteria on the germination of cicer arietinum seeds and seedling growth, vol. I(1). J. Herb. Med.Toxicol, 2007, pp. 61 – 63.
[28]	C. S. Nautiyal, An efficient microbiological growth medium for screening phosphate solubilizing microorganisms, vol.170. FEMS Microbiol. Letters, 1999. pp. 265–70.
[29]	R. Gupta, S. Rekha, S. Aparna. and R.C. Kuhad, A modified plate assay for screening phosphate solubilizing microorganisms, vol. 40. J. Gen. Appl. Microbiol, 1994, pp. 255–260.
[30]	El-Azeem, S.A.M., T. A. Mehana and A. A. Shabayek, Some plant growth promoting traits rhizobacteria isolated from Suez Canal region, Egypt, vol. 8. African Crop Science Society. Proceedings, 2007, pp.1517-1525.
[31]	A. E. Richardson, J. M. Barea, A. M. McNeill, C. Prigent-Combaret, Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms, vol. 321. Plant Soil, 2009, pp. 305–339.
[32]	D. V. Guebel and N. V. T. Darias, Optimization of the citric acid production by Aspergillus niger through a metabolic Flux balance model , vol. 4. J. Biotechnol, 2001, pp.1-12.

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APA Style

Muh Jayadi, Baharuddin, Bachrul Ibrahim. (2013). In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia. American Journal of Agriculture and Forestry, 1(4), 68-73. https://doi.org/10.11648/j.ajaf.20130104.14

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ACS Style

Muh Jayadi; Baharuddin; Bachrul Ibrahim. In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia. Am. J. Agric. For. 2013, 1(4), 68-73. doi: 10.11648/j.ajaf.20130104.14

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AMA Style

Muh Jayadi, Baharuddin, Bachrul Ibrahim. In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia. Am J Agric For. 2013;1(4):68-73. doi: 10.11648/j.ajaf.20130104.14

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@article{10.11648/j.ajaf.20130104.14,
  author = {Muh Jayadi and Baharuddin and Bachrul Ibrahim},
  title = {In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia},
  journal = {American Journal of Agriculture and Forestry},
  volume = {1},
  number = {4},
  pages = {68-73},
  doi = {10.11648/j.ajaf.20130104.14},
  url = {https://doi.org/10.11648/j.ajaf.20130104.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20130104.14},
  abstract = {Phosphorus (P) is the second most required nutrient of plants, but its availability in ultisols is relatively low. Source of P can be found from rock phosphate (RP) which has low solubility level. Use of microbial solubilizing phosphate such as bacteria, fungi or combination of both is potential to increase availability of P. This research was aimed to find bacterial or fungal isolates that are high potential in solubilizing of RP. In vitro selection was conducted to obtain indigenous bacteria or fungi from Ultisols of South Sulawesi. Selection of microbes for their capability to solubate rock phosphate qualitatively, conducted through measuring the solubility index (SI) and solubility efficiency (SE) on pykovskaya solid medium and on National Botanical Research Institutes phosphate-bromophenol blue (NBRIP-BPB) medium. The results of this study indicated that from SI and SE highest values, four high potential isolates were selected namely, Aspergillus niger JTM 2, Bacillus sp JTM 3, Aspergillus niger JTM 6 and Pseudomonas sp JTM 10. Ability of solubilizing phosphate from the four isolates was then tested in liquid medium pikovskaya either in single or mixed combination. Solubilizing phosphate of RF was determined according to [1] method. The results of test on solid media indicate that Bacillus sp JTM 3 and Pseudomonas sp JTM 10 have SI and SE higher than both Aspergillus niger JTM 2 and Aspergillus niger JTM 6. Futhermore, test on Pikovskaya liquid media confirmed that Aspergillus niger JTM 6 and Pseudomonas sp JTM 10 gives the higher results than other single isolates and control, whereas the combination of isolates Bacillus sp JTM 3 and Aspergillus niger JTM 2 indicated significantly higher solubilizing phosphorus production than all other treatments.},
 year = {2013}
}

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TY  - JOUR
T1  - In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia
AU  - Muh Jayadi
AU  - Baharuddin
AU  - Bachrul Ibrahim
Y1  - 2013/12/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajaf.20130104.14
DO  - 10.11648/j.ajaf.20130104.14
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 68
EP  - 73
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20130104.14
AB  - Phosphorus (P) is the second most required nutrient of plants, but its availability in ultisols is relatively low. Source of P can be found from rock phosphate (RP) which has low solubility level. Use of microbial solubilizing phosphate such as bacteria, fungi or combination of both is potential to increase availability of P. This research was aimed to find bacterial or fungal isolates that are high potential in solubilizing of RP. In vitro selection was conducted to obtain indigenous bacteria or fungi from Ultisols of South Sulawesi. Selection of microbes for their capability to solubate rock phosphate qualitatively, conducted through measuring the solubility index (SI) and solubility efficiency (SE) on pykovskaya solid medium and on National Botanical Research Institutes phosphate-bromophenol blue (NBRIP-BPB) medium. The results of this study indicated that from SI and SE highest values, four high potential isolates were selected namely, Aspergillus niger JTM 2, Bacillus sp JTM 3, Aspergillus niger JTM 6 and Pseudomonas sp JTM 10. Ability of solubilizing phosphate from the four isolates was then tested in liquid medium pikovskaya either in single or mixed combination. Solubilizing phosphate of RF was determined according to [1] method. The results of test on solid media indicate that Bacillus sp JTM 3 and Pseudomonas sp JTM 10 have SI and SE higher than both Aspergillus niger JTM 2 and Aspergillus niger JTM 6. Futhermore, test on Pikovskaya liquid media confirmed that Aspergillus niger JTM 6 and Pseudomonas sp JTM 10 gives the higher results than other single isolates and control, whereas the combination of isolates Bacillus sp JTM 3 and Aspergillus niger JTM 2 indicated significantly higher solubilizing phosphorus production than all other treatments.
VL  - 1
IS  - 4
ER  -

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Author Information

Muh Jayadi

Department of Soil Science, Hasanuddin University, Makassar, Indonesia
Baharuddin

Research Center for Biotechnology, Hasanuddin University, Makassar, Indonesia
Bachrul Ibrahim

Department of Soil Science, Hasanuddin University, Makassar, Indonesia

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APA Style

Muh Jayadi, Baharuddin, Bachrul Ibrahim. (2013). In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia. American Journal of Agriculture and Forestry, 1(4), 68-73. https://doi.org/10.11648/j.ajaf.20130104.14

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ACS Style

Muh Jayadi; Baharuddin; Bachrul Ibrahim. In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia. Am. J. Agric. For. 2013, 1(4), 68-73. doi: 10.11648/j.ajaf.20130104.14

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AMA Style

Muh Jayadi, Baharuddin, Bachrul Ibrahim. In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia. Am J Agric For. 2013;1(4):68-73. doi: 10.11648/j.ajaf.20130104.14

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@article{10.11648/j.ajaf.20130104.14,
  author = {Muh Jayadi and Baharuddin and Bachrul Ibrahim},
  title = {In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia},
  journal = {American Journal of Agriculture and Forestry},
  volume = {1},
  number = {4},
  pages = {68-73},
  doi = {10.11648/j.ajaf.20130104.14},
  url = {https://doi.org/10.11648/j.ajaf.20130104.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20130104.14},
  abstract = {Phosphorus (P) is the second most required nutrient of plants, but its availability in ultisols is relatively low. Source of P can be found from rock phosphate (RP) which has low solubility level. Use of microbial solubilizing phosphate such as bacteria, fungi or combination of both is potential to increase availability of P. This research was aimed to find bacterial or fungal isolates that are high potential in solubilizing of RP. In vitro selection was conducted to obtain indigenous bacteria or fungi from Ultisols of South Sulawesi. Selection of microbes for their capability to solubate rock phosphate qualitatively, conducted through measuring the solubility index (SI) and solubility efficiency (SE) on pykovskaya solid medium and on National Botanical Research Institutes phosphate-bromophenol blue (NBRIP-BPB) medium. The results of this study indicated that from SI and SE highest values, four high potential isolates were selected namely, Aspergillus niger JTM 2, Bacillus sp JTM 3, Aspergillus niger JTM 6 and Pseudomonas sp JTM 10. Ability of solubilizing phosphate from the four isolates was then tested in liquid medium pikovskaya either in single or mixed combination. Solubilizing phosphate of RF was determined according to [1] method. The results of test on solid media indicate that Bacillus sp JTM 3 and Pseudomonas sp JTM 10 have SI and SE higher than both Aspergillus niger JTM 2 and Aspergillus niger JTM 6. Futhermore, test on Pikovskaya liquid media confirmed that Aspergillus niger JTM 6 and Pseudomonas sp JTM 10 gives the higher results than other single isolates and control, whereas the combination of isolates Bacillus sp JTM 3 and Aspergillus niger JTM 2 indicated significantly higher solubilizing phosphorus production than all other treatments.},
 year = {2013}
}

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TY  - JOUR
T1  - In Vitro Selection of Rock Phosphate Solubility by Microorganism from Ultisols in South Sulawesi, Indonesia
AU  - Muh Jayadi
AU  - Baharuddin
AU  - Bachrul Ibrahim
Y1  - 2013/12/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajaf.20130104.14
DO  - 10.11648/j.ajaf.20130104.14
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 68
EP  - 73
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20130104.14
AB  - Phosphorus (P) is the second most required nutrient of plants, but its availability in ultisols is relatively low. Source of P can be found from rock phosphate (RP) which has low solubility level. Use of microbial solubilizing phosphate such as bacteria, fungi or combination of both is potential to increase availability of P. This research was aimed to find bacterial or fungal isolates that are high potential in solubilizing of RP. In vitro selection was conducted to obtain indigenous bacteria or fungi from Ultisols of South Sulawesi. Selection of microbes for their capability to solubate rock phosphate qualitatively, conducted through measuring the solubility index (SI) and solubility efficiency (SE) on pykovskaya solid medium and on National Botanical Research Institutes phosphate-bromophenol blue (NBRIP-BPB) medium. The results of this study indicated that from SI and SE highest values, four high potential isolates were selected namely, Aspergillus niger JTM 2, Bacillus sp JTM 3, Aspergillus niger JTM 6 and Pseudomonas sp JTM 10. Ability of solubilizing phosphate from the four isolates was then tested in liquid medium pikovskaya either in single or mixed combination. Solubilizing phosphate of RF was determined according to [1] method. The results of test on solid media indicate that Bacillus sp JTM 3 and Pseudomonas sp JTM 10 have SI and SE higher than both Aspergillus niger JTM 2 and Aspergillus niger JTM 6. Futhermore, test on Pikovskaya liquid media confirmed that Aspergillus niger JTM 6 and Pseudomonas sp JTM 10 gives the higher results than other single isolates and control, whereas the combination of isolates Bacillus sp JTM 3 and Aspergillus niger JTM 2 indicated significantly higher solubilizing phosphorus production than all other treatments.
VL  - 1
IS  - 4
ER  -

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