Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam

Nguyen Khoi Nghia; Tran Thi My Tien; Nguyen Thi Kieu Oanh; Nguyen Hoang Kim Nuong

doi:doi:10.11648/j.aff.20170603.11

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Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam

Nguyen Khoi Nghia, Tran Thi My Tien, Nguyen Thi Kieu Oanh, Nguyen Hoang Kim Nuong

Published in Agriculture, Forestry and Fisheries (Volume 6, Issue 3)

Received: 4 April 2017 Accepted: 13 April 2017 Published: 17 May 2017

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Abstract

The main aim of this study was to deal with isolation, characterization and identification IAA producing bacteria from paddy rice cultivated soil samples collected in rice–shrimp farming system in salt affected areas of the Mekong Delta of Vietnam. Fifteen soil samples collected from the rice crop in the rice-shrimp farming system of 5 different provinces within the Mekong Delta, Vietnam were used to isolate the IAA producing bacteria. NBRIP media was used to isolate the potentially IAA producing bacteria. Result showed that 213 isolates were obtained from the fifteen different soil samples. Out of 45 indole acetic acid producing isolates, ten were selected as efficient producers. One out of ten, the isolate ST2-1 was identified as the most promising strain as efficient biofertilizer inoculants to promote plant growth. This strain produced 33.13 mg.L^-1 as the highest concentration of IAA after 8 days of incubation. As well, this strain stimulated the growth of rice’s roots and dry biomass after 7 days of experiment with Hoagland medium containing 0.3% NaCl in 100 mL glass tube. This strain produced well IAA production in pH variation range of medium between 5 and 9 and this bacterial strain can resist to salinity of environment up to 3% NaCl. However, this bacterial was highly inhibited by not only 3 commonly used antibiotics: Ampicillin, Chloramphenicol and Steptomycin at recommended rates but also 3 plant pathology fungicides: Thiophanate Methyl, Propineb and Mancozed regarding to IAA production ability. The results of the 16S rRNA gene sequence analysis showed that this IAA producer coded as ST2-1 was genetically identified as species of Bacillus megaterium ST2-9 since 99% of its sequence affiliated with Bacillus megaterium. In conclusion, the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.

Published in	Agriculture, Forestry and Fisheries (Volume 6, Issue 3)
DOI	10.11648/j.aff.20170603.11
Page(s)	69-77
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Bacillus Megaterium, Bacteria, Indole-3-Acetic Acid (IAA), Rice–Shrimp Farming System, Salt Affected Soil and 16S rRNA

References

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Nguyen Khoi Nghia, Tran Thi My Tien, Nguyen Thi Kieu Oanh, Nguyen Hoang Kim Nuong. (2017). Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agriculture, Forestry and Fisheries, 6(3), 69-77. https://doi.org/10.11648/j.aff.20170603.11

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Nguyen Khoi Nghia; Tran Thi My Tien; Nguyen Thi Kieu Oanh; Nguyen Hoang Kim Nuong. Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agric. For. Fish. 2017, 6(3), 69-77. doi: 10.11648/j.aff.20170603.11

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AMA Style

Nguyen Khoi Nghia, Tran Thi My Tien, Nguyen Thi Kieu Oanh, Nguyen Hoang Kim Nuong. Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agric For Fish. 2017;6(3):69-77. doi: 10.11648/j.aff.20170603.11

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@article{10.11648/j.aff.20170603.11,
  author = {Nguyen Khoi Nghia and Tran Thi My Tien and Nguyen Thi Kieu Oanh and Nguyen Hoang Kim Nuong},
  title = {Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {6},
  number = {3},
  pages = {69-77},
  doi = {10.11648/j.aff.20170603.11},
  url = {https://doi.org/10.11648/j.aff.20170603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20170603.11},
  abstract = {The main aim of this study was to deal with isolation, characterization and identification IAA producing bacteria from paddy rice cultivated soil samples collected in rice–shrimp farming system in salt affected areas of the Mekong Delta of Vietnam. Fifteen soil samples collected from the rice crop in the rice-shrimp farming system of 5 different provinces within the Mekong Delta, Vietnam were used to isolate the IAA producing bacteria. NBRIP media was used to isolate the potentially IAA producing bacteria. Result showed that 213 isolates were obtained from the fifteen different soil samples. Out of 45 indole acetic acid producing isolates, ten were selected as efficient producers. One out of ten, the isolate ST2-1 was identified as the most promising strain as efficient biofertilizer inoculants to promote plant growth. This strain produced 33.13 mg.L-1 as the highest concentration of IAA after 8 days of incubation. As well, this strain stimulated the growth of rice’s roots and dry biomass after 7 days of experiment with Hoagland medium containing 0.3% NaCl in 100 mL glass tube. This strain produced well IAA production in pH variation range of medium between 5 and 9 and this bacterial strain can resist to salinity of environment up to 3% NaCl. However, this bacterial was highly inhibited by not only 3 commonly used antibiotics: Ampicillin, Chloramphenicol and Steptomycin at recommended rates but also 3 plant pathology fungicides: Thiophanate Methyl, Propineb and Mancozed regarding to IAA production ability. The results of the 16S rRNA gene sequence analysis showed that this IAA producer coded as ST2-1 was genetically identified as species of Bacillus megaterium ST2-9 since 99% of its sequence affiliated with Bacillus megaterium. In conclusion, the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.},
 year = {2017}
}

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TY  - JOUR
T1  - Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam
AU  - Nguyen Khoi Nghia
AU  - Tran Thi My Tien
AU  - Nguyen Thi Kieu Oanh
AU  - Nguyen Hoang Kim Nuong
Y1  - 2017/05/17
PY  - 2017
N1  - https://doi.org/10.11648/j.aff.20170603.11
DO  - 10.11648/j.aff.20170603.11
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 69
EP  - 77
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20170603.11
AB  - The main aim of this study was to deal with isolation, characterization and identification IAA producing bacteria from paddy rice cultivated soil samples collected in rice–shrimp farming system in salt affected areas of the Mekong Delta of Vietnam. Fifteen soil samples collected from the rice crop in the rice-shrimp farming system of 5 different provinces within the Mekong Delta, Vietnam were used to isolate the IAA producing bacteria. NBRIP media was used to isolate the potentially IAA producing bacteria. Result showed that 213 isolates were obtained from the fifteen different soil samples. Out of 45 indole acetic acid producing isolates, ten were selected as efficient producers. One out of ten, the isolate ST2-1 was identified as the most promising strain as efficient biofertilizer inoculants to promote plant growth. This strain produced 33.13 mg.L-1 as the highest concentration of IAA after 8 days of incubation. As well, this strain stimulated the growth of rice’s roots and dry biomass after 7 days of experiment with Hoagland medium containing 0.3% NaCl in 100 mL glass tube. This strain produced well IAA production in pH variation range of medium between 5 and 9 and this bacterial strain can resist to salinity of environment up to 3% NaCl. However, this bacterial was highly inhibited by not only 3 commonly used antibiotics: Ampicillin, Chloramphenicol and Steptomycin at recommended rates but also 3 plant pathology fungicides: Thiophanate Methyl, Propineb and Mancozed regarding to IAA production ability. The results of the 16S rRNA gene sequence analysis showed that this IAA producer coded as ST2-1 was genetically identified as species of Bacillus megaterium ST2-9 since 99% of its sequence affiliated with Bacillus megaterium. In conclusion, the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.
VL  - 6
IS  - 3
ER  -

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Author Information

Nguyen Khoi Nghia

Department of Soil Science, College of Agriculture and Applied Biology, Cantho University, Cantho City, Vietnam
Tran Thi My Tien

Department of Soil Science, College of Agriculture and Applied Biology, Cantho University, Cantho City, Vietnam
Nguyen Thi Kieu Oanh

Department of Soil Science, College of Agriculture and Applied Biology, Cantho University, Cantho City, Vietnam
Nguyen Hoang Kim Nuong

Department of Soil Science, College of Agriculture and Applied Biology, Cantho University, Cantho City, Vietnam

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Nguyen Khoi Nghia, Tran Thi My Tien, Nguyen Thi Kieu Oanh, Nguyen Hoang Kim Nuong. (2017). Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agriculture, Forestry and Fisheries, 6(3), 69-77. https://doi.org/10.11648/j.aff.20170603.11

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ACS Style

Nguyen Khoi Nghia; Tran Thi My Tien; Nguyen Thi Kieu Oanh; Nguyen Hoang Kim Nuong. Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agric. For. Fish. 2017, 6(3), 69-77. doi: 10.11648/j.aff.20170603.11

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AMA Style

Nguyen Khoi Nghia, Tran Thi My Tien, Nguyen Thi Kieu Oanh, Nguyen Hoang Kim Nuong. Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agric For Fish. 2017;6(3):69-77. doi: 10.11648/j.aff.20170603.11

Copy | Download

@article{10.11648/j.aff.20170603.11,
  author = {Nguyen Khoi Nghia and Tran Thi My Tien and Nguyen Thi Kieu Oanh and Nguyen Hoang Kim Nuong},
  title = {Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {6},
  number = {3},
  pages = {69-77},
  doi = {10.11648/j.aff.20170603.11},
  url = {https://doi.org/10.11648/j.aff.20170603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20170603.11},
  abstract = {The main aim of this study was to deal with isolation, characterization and identification IAA producing bacteria from paddy rice cultivated soil samples collected in rice–shrimp farming system in salt affected areas of the Mekong Delta of Vietnam. Fifteen soil samples collected from the rice crop in the rice-shrimp farming system of 5 different provinces within the Mekong Delta, Vietnam were used to isolate the IAA producing bacteria. NBRIP media was used to isolate the potentially IAA producing bacteria. Result showed that 213 isolates were obtained from the fifteen different soil samples. Out of 45 indole acetic acid producing isolates, ten were selected as efficient producers. One out of ten, the isolate ST2-1 was identified as the most promising strain as efficient biofertilizer inoculants to promote plant growth. This strain produced 33.13 mg.L-1 as the highest concentration of IAA after 8 days of incubation. As well, this strain stimulated the growth of rice’s roots and dry biomass after 7 days of experiment with Hoagland medium containing 0.3% NaCl in 100 mL glass tube. This strain produced well IAA production in pH variation range of medium between 5 and 9 and this bacterial strain can resist to salinity of environment up to 3% NaCl. However, this bacterial was highly inhibited by not only 3 commonly used antibiotics: Ampicillin, Chloramphenicol and Steptomycin at recommended rates but also 3 plant pathology fungicides: Thiophanate Methyl, Propineb and Mancozed regarding to IAA production ability. The results of the 16S rRNA gene sequence analysis showed that this IAA producer coded as ST2-1 was genetically identified as species of Bacillus megaterium ST2-9 since 99% of its sequence affiliated with Bacillus megaterium. In conclusion, the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam
AU  - Nguyen Khoi Nghia
AU  - Tran Thi My Tien
AU  - Nguyen Thi Kieu Oanh
AU  - Nguyen Hoang Kim Nuong
Y1  - 2017/05/17
PY  - 2017
N1  - https://doi.org/10.11648/j.aff.20170603.11
DO  - 10.11648/j.aff.20170603.11
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 69
EP  - 77
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20170603.11
AB  - The main aim of this study was to deal with isolation, characterization and identification IAA producing bacteria from paddy rice cultivated soil samples collected in rice–shrimp farming system in salt affected areas of the Mekong Delta of Vietnam. Fifteen soil samples collected from the rice crop in the rice-shrimp farming system of 5 different provinces within the Mekong Delta, Vietnam were used to isolate the IAA producing bacteria. NBRIP media was used to isolate the potentially IAA producing bacteria. Result showed that 213 isolates were obtained from the fifteen different soil samples. Out of 45 indole acetic acid producing isolates, ten were selected as efficient producers. One out of ten, the isolate ST2-1 was identified as the most promising strain as efficient biofertilizer inoculants to promote plant growth. This strain produced 33.13 mg.L-1 as the highest concentration of IAA after 8 days of incubation. As well, this strain stimulated the growth of rice’s roots and dry biomass after 7 days of experiment with Hoagland medium containing 0.3% NaCl in 100 mL glass tube. This strain produced well IAA production in pH variation range of medium between 5 and 9 and this bacterial strain can resist to salinity of environment up to 3% NaCl. However, this bacterial was highly inhibited by not only 3 commonly used antibiotics: Ampicillin, Chloramphenicol and Steptomycin at recommended rates but also 3 plant pathology fungicides: Thiophanate Methyl, Propineb and Mancozed regarding to IAA production ability. The results of the 16S rRNA gene sequence analysis showed that this IAA producer coded as ST2-1 was genetically identified as species of Bacillus megaterium ST2-9 since 99% of its sequence affiliated with Bacillus megaterium. In conclusion, the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.
VL  - 6
IS  - 3
ER  -

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